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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26544249 - 03/19/20 09:12 AM (3 years, 10 months ago)

Which is what I sort of tried to do before! So this time I have just mixed some coir, verm, pine needles, pine cone pieces, dry leaves and not added the worm castings. They seemed to like that too much last time.
I have also left the mix fairly dry since I already put a damp layer of verm last night. Let's see if they will take the hint that its fruiting time or if they will just continue to eat anything I put in the box haha


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26544314 - 03/19/20 09:44 AM (3 years, 10 months ago)

Quote:

Zenn said:
Let's see if they will take the hint that its fruiting time or if they will just continue to eat anything I put in the box haha




My thoughts...
Cover mycelium and continue Phase Spawn Run:
Incubation Temperature: 65-75° F. (18-24° C.)
Relative Humidity: 95-100%
FreshAir Exchanges: 0-1 volume per hour
Light Requirements: without light

When mycelium will cover 30-40% of substrate surface activate Phase Primordia Formation


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26545644 - 03/19/20 10:46 PM (3 years, 10 months ago)

Thank you :smile:

I will add that it has actually been in the cooler temp range for the last 2 weeks, so I was surprised that it colonised the original casing so fast.
How would moving it back to spawn run temps effect it now it's been between primordia/fruit temps for about 2 and a half weeks?
Here is how it looked on 1st of March when I gave it the worm cast/mulch casing:




Edited by Zenn (03/19/20 11:05 PM)


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26545827 - 03/20/20 03:01 AM (3 years, 10 months ago)

Quote:

it has actually been in the cooler temp range for the last 2 weeks



Oh. My plan is no good then.
As you see, mycelium on photo in your first post and mycelium on March 1st photo are different. Time to switch from spawn run to primordia formation. Low temp and FAE


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Edited by Hamsabala (03/20/20 03:42 AM)


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26545877 - 03/20/20 05:03 AM (3 years, 10 months ago)

It's ok. That's what I thought since if ended up fully colonizing the last layer even with the cooler temps. So have left it in the room with the window. Last night it got very cold 3.3°C so this may help with the addition of new top layer. *crosses fingers* hopefully it might see the new drier (but not dry) layer is the surface. I guess we now wait and see...
Will leave it another day or 2 before I mist new layer.


Edited by Zenn (03/20/20 05:05 AM)


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26545964 - 03/20/20 07:04 AM (3 years, 10 months ago)

i am watching with huge interest and wish you luck

Quote:

... and Psilocybe semilanceata indoor started on cakes.



How is going with that ones?


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26545991 - 03/20/20 07:19 AM (3 years, 10 months ago)

Quote:

Hamsabala said:
i am watching with huge interest and wish you luck

Quote:

... and Psilocybe semilanceata indoor started on cakes.



How is going with that ones?




https://www.shroomery.org/forums/showflat.php/Number/26541144

They are only 1 and a half weeks old so far, but a little bigger and a little whiter than the other day. :smile:


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26551745 - 03/22/20 08:38 PM (3 years, 10 months ago)

After the new drier top layer the other day, we seem to have some fuzz trying to come up round the edges



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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26552149 - 03/23/20 12:09 AM (3 years, 10 months ago)

Hungry mycelium


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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26568641 - 03/31/20 08:45 AM (3 years, 10 months ago)

Pic update.:



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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26569116 - 03/31/20 01:17 PM (3 years, 10 months ago)

Great progress
What is your next step?


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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26580231 - 04/05/20 06:56 PM (3 years, 10 months ago)

Well I have it in the pantry still, next to open window. It is coldest room as it is same temps as outdoors. Recently the highest daytime (today) was about 15.5°c and tonight it is 14°C (warmer than usual). to be fair I had tried to start fruiting phase when I started this thread lol. But we added that extra layer...and it has eaten through it again...even though it has been in low temps. To be honest I dont rightly know what else to do at this stage apart from wait and see what happens. Temps and humidity are usually in the lower range so right temps for primordial conditions... so maybe I try upping fanning a little


Edited by Zenn (04/05/20 07:01 PM)


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26581811 - 04/06/20 01:59 PM (3 years, 10 months ago)

I have read an interesting grow report about Ps. Cyanescens. It is translated on Russian and i haven't found the original. It says there - do 24 hours cold shock (2-5 °С) before fruiting, make 10+°С after.


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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26581965 - 04/06/20 03:26 PM (3 years, 10 months ago)

Good to know thank you. I did try to do this when I started this thread. Used the cold room (it was colder then) and an ice mist, but since adding another layer etc it probably wouldn't hurt to try again. I wonder if laying 6+ ice cubes on the surface could help mimic a frosty evening? Luckily the temperature has dropped back to 10°C this evening. Maybe lower later.

Pretty sure it has practically fully colonised the top now as it is sooo white. Very hungry mycelium just eats anything I have put down. I'm curious if trying vermiculite layer again could help ...as I dont think it would eat that... but at this rate who knows haha


Edited by Zenn (04/06/20 03:28 PM)


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26583186 - 04/07/20 03:08 AM (3 years, 10 months ago)

Short try
2cm soil layer - cold shock - fruiting

Long try
Soil - grass seeds - verm - wait till grass will grow - cold shock - fruiting

What do you think?


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26583777 - 04/07/20 11:16 AM (3 years, 10 months ago)

Hmm... I think definitely cold shock. But every time I add soil or coir or new mulch it just eats it.
The grass seed is interesting idea, using that sort of method for my Ps. Semilanceata.


I have put a fairly thin layer of verm on the top of the mycelium, in the hop this will help as it is not nutritious and helps keep moisture on the surface. If there is no food left for it, it will hopefully think "ok, full colonisation complete...". Full colonisation is the first trigger for fruiting.

I did this thin layer of verm on a Ps. Cubensis  (GT) tub and it was successful. So maybe?

I had to move it off the window ledge to the cold stone floor as the sun was quite warm coming through the window today.


I have a second side to the Cyanescens project... I have attempted to save my other box. A few weeks back I cut out the healthiest part and broke it up into new box, picking out any new infection I found. It healed well. The growth is very different and more like the usual cyanescens fingers/rhizo. Last night I checked the old tub. Big part was black and hairy! Never seen black hair like that before! But I cut it aside and I find SO MUCH HEALTHY RHIZO MYCELIUM since last time. So I attempted surgery to remove as much infected bits while breaking the "clean" myc up and mixing it with the other healthy part I saved. The house Reeked of mushroom last night from the surgery hahaha!


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26583830 - 04/07/20 11:45 AM (3 years, 10 months ago)

i hope you have some mycelium in fridge for preserving ))


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Hamsabala]
    #26586673 - 04/08/20 03:28 PM (3 years, 10 months ago)

I have a very clear Liquid culture in the fridge as well as some agar germinated spores.

Today I initiate cold shock for this as the box JUST fits in my fridge :smile: (just to be clear this box is nice and healthy. No contamination)

The second box I will check in a day or 2 for recovery and infection after the "surgery" and transplanting.


Edited by Zenn (04/08/20 03:31 PM)


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OfflineZenn
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26599240 - 04/13/20 10:59 PM (3 years, 9 months ago)

I'd like to add to this thread that part of the inspiration for the used methods was from an indoor grow for Ps. Azurescens

https://www.shroomery.org/8702/Psilocybe-azurescens-indoor-cultivation

As well as 2005 Waylit Jim Ps. Cyanescens grows.


As for progress report, we are just waiting now after applying the wet vermiculite. We likely gonna be waiting a little while before we see anything.


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OfflineHamsabala
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Re: Ps. Cyanescens mycelium in mulch box, so far [Re: Zenn]
    #26600688 - 04/14/20 02:22 PM (3 years, 9 months ago)

:popcorn:


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