Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Offlineeagle12
Stranger
Registered: 04/11/19
Posts: 64
Last seen: 3 years, 6 months
First time working with agar agar plates need help
    #26600125 - 04/14/20 10:49 AM (3 years, 9 months ago)

I used 8 gs agar agar 6gs brf 400ml water pc at 15 psi for 25 min then poored into pint wide mouth masons and pc again for 20 min....i had growth pop up on day 3 and now its day 7 with all 11 jars showin signs of growth but most are the size of dime...so now my question is how long should it take to see more growth? Would cold nights at 59 degrees stall my plates? Any help will be great.


Extras: Filter Print Post Top
Offlinecli_hlt
Beshroomed
Male User Gallery


Registered: 10/29/11
Posts: 462
Loc: EU
Last seen: 1 month, 9 days
Re: First time working with agar agar plates need help [Re: eagle12]
    #26600139 - 04/14/20 10:57 AM (3 years, 9 months ago)

59°F is a bit chilly for cubes. (I guess you're trying to grow cubes.) Dime-ized cultures at 7 days is not that bad considering the cold. If you're planning to do transfers, you can do that already. To drop some wedges to grain, you'd ideally need a bit more mycelium.


Extras: Filter Print Post Top
Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: First time working with agar agar plates need help [Re: cli_hlt]
    #26602621 - 04/15/20 10:39 AM (3 years, 9 months ago)

Okay so I've been working with agar for a couple weeks and here's what I've learned so far:
Sterilization and sanitation are of #1 importance. I've had spore overtaken by bacteria within literal hours. Keep your agar mixes simple, 10g agar 8g Malt Extract, I also like Potato Dextrose agar, if you prepare rye grain you can use your soak water after its been boiled as nutrient which makes it as simple as adding 10g of agar to 500ml of your rye soak.

I commend your process of pressure cooking, however if you condense the pc into a singular step it will be easier for you as you're using mason jars instead of plastic petri dishes (they are popular but flimsy whereas mason jars you don't pour, removing an avenue of contamination see: https://www.shroomery.org/forums/showflat.php/Number/26279439

I have found through trial and error that 69f (nice) to 73f produces quick myc growth. I would give all your agar plates 8 days to colonize before transferring to a new, cleaner plate. Hope I helped!


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 12,184 3 12/04/99 12:35 AM
by Anonymous
* Whats with my agar plates? Frankenstein 8,594 8 09/08/22 03:46 PM
by schpat
* How many mycelium syringes from one agar plate? BBShrizz 4,882 3 05/10/01 02:10 AM
by ISaToriBluEI
* Re: agar plate question Psilowarrior 2,541 2 12/10/99 02:09 PM
by rebelmoon
* Spore Syringe on Agar plates Heruuka 2,929 2 09/16/02 06:31 AM
by Anno
* Benefits of Agar? celsius 9,381 10 12/01/19 12:36 AM
by wildernessjunkie
* Agar and growing that white stuff! pj541 2,507 5 10/15/01 04:06 PM
by pj541
* Re: Plate Count Agar? Is this ready to use? shroomeritus 2,048 8 04/24/01 07:53 PM
by shroomeritus

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
106 topic views. 22 members, 229 guests and 72 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.009 seconds on 14 queries.