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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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b+,toc,pesa print, a2a, a2g, coir/verm, mp mono [COMPLETE][contam city]
#26595905 - 04/12/20 02:26 PM (3 years, 9 months ago) |
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I'm doing a grow and wanted to share. I have 3 strains, verities, types? idk they're all cubes.
I first started by streaking each print onto agar.
I then did 1 transfer to a bunch of plates, picking spots that I thought had the best growth.
 
During this time I also streaked the TOC and PESA but I dont have pics of the streak plates early on.
 
All the plates a couple days later
 

I dropped 2 plates of the b+ t1 onto wbs which I soaked for 24 or so hours (it was starting to ferment bad, after this run I only do 12 or so hours now.) I cut 2 plates into 2 pizzas, 16 slices, cutting away the outside rim of the plate, then dropped 1 slice per jar.
Here is how they look today, these were dropped 4-10.

I also dropped a plate of TOC that looked really good into 9 jars, I cut one slice in half. I think this pic is the plate I used, if not it looked similar.
B+ jars

----------------UPDATE---------------- 4-20-20
 I shook all these a few mins ago. Idk if its too soon or not, but it seemed fine.
This one has more growth than the rest, it looks similar though.
   Now its time to wait for regrowth!
Here are the TOC jars today, I ended up shaking these the next day(4-21)
  I tossed the jar with a lot of growth. I saw a small dark spot that looked out of place, turns out it was on the outside of the glass It still looked a little weird, 12:00 on the big white mass you can sort of see it. Also the moisture looked high.
----------UPDATE---------- 4-23-20 Here are the jars today, lots of regrowth!


These jars are regrowing super fast!
 
---------UPDATE--------- 4-27
a couple tiny spots not finshed, mostly colonized B+.
  

other B+ jars coming along nicely.

I forgot to mention I dropped a PESA plate on 4-18, here it is today. TOC jars on top, PESA just shaken on bottom. I didn't get a pic of the jars before I shook them, they were about the same as when I shook the B+.

one TOC jar is nearly 100% while none others are near there. it looks good as far as I can tell, I can try to get a better pic if needed.

It was also at this time I saw one of the jars had some growth inside it that didn't match all the other growth. Rather than nice uniform rhizo growth it was thin and whispy, and filled all the parts of the jar that were uncolonized. This has to be cobweb. Sorry I didn't take a pic. I took the jar outside and disposed of the contents in a spot far away. See below for another jar that came out the same way as this.
--------UPDATE-------- 4-30
These are my first bulk tubs. I spawned these last night, forgot to take pics of the process. I dropped a jar being impatient and had to clean up glass shards, the spawn only broke in half and some off the sides so I still used it.
 
I did mediocre on leveling the surface, and I'm almost certain I made the casing layer too thick. Its only 3/4" max in spots, but I think it will do fine. If I run into stalling problems I will scrape it with a fork to try to get some of the casing off.
I used 4 colonized jars, 1 brick coir(close to 650g), 2 qts verm, 4 qts water each. I realized I forgot to case, so I used half the ingredients and made a layer on the 2 tubs. I also ended up not using a liner for these 2. It seemed like such a pita, but I will give it another try when I spawn the next tub.
These are my other B+ jars almost ready!
   
Here is another jar that is showing what I think is cobweb.
 
Here is a jar from the same batch, without any wispy growth.

--------UPDATE-------- 5-1
I know its 5-2 but I took these pics last night.

I spawned the 2 tubs on the left on 5-1 using these jars. I forgot to add a casing. I might add a late one, or just not. I also tried the liner on these 2 tubs.
(the 4 on the left)
 (the 4 on the right)

Here's an update on growth on the other 2.
 
--------------UPDATE-------------- 5-3
Here are the B+ tubs I'm guessing I will have to scrape these in a couple days to get some casing off. I think the little bit of growth was just seeds that got pushed to the top when leveling, and that I will run into stalling issues. Any tips help.
(casing no liner)

(liner no casing)

And here's the TOC tub, liner, not cased.

These tubs are 2 days old!
I made some shoe boxes today, doing a little experimenting.

 
I took 2 jars of each, b+ and toc, and used half the coir/verm/water as the monos which require 4 jars, and mixed them all up in 2 different buckets. I then filled shoe boxes and leveled them and found I could make 4 if I made them a little thinner, not sure how this will go.
I will open each shoe box and put a bag with small holes over them probably tomorrow, figured they will be fine with the lids for a couple days, not sure about the FAE.
-------------UPDATE-------------- 5-11
I did the thing with the bags, and made a couple more shoe boxes. If you look on the top shelf you see I also transferred all my dishes to t3 from germ.
 
Here is the B+ tub that was cased too thick. I ended up scraping off the top of the sub with a fork that I washed like any other dish. I tipped the tub on a slight angle and forked a lot of the loose top layer off, pushing it to the lower end, then scoop/flung the piled up casing along the tub wall into another spare tub. Here it is a few days later.
 
The B+ tub from 5-1
 
And the toc tub from 5-1.
 
Here is the PESA about to go into a mono tomorrow most likely.

These 4 arent far behind.
 
-------------UPDATE-------------- 5-16
I spawned these 2 pesa tubs on 5-12 I tried to case a lot thinner, and didn't use a liner.
 
B+ tubs spawned 4-27 You know, the ones that I scraped most of the casing off of. These pics below are all the same tub
    Here is the other b+ tub from the same time
 
Here is the TOC tub spawned 5-1
 
Here is the b+ tub spawned 5-1
 
And an update on the shoe boxes. Not sure which is which I took too many pics. They're only a day apart from each other, and they're either b+ or TOC. I have them labeled I just wasn't keeping track of pics.

--------------UPDATE-------------- 5-18
PINS!!
I'm not sure if this looks healthy or not. Some weird black stuff right by the pins, and the weird colored blob to the right and down from the pins.
 
These ones look better

There is some weird colors in this tub that worry me. I am having trouble uploading pics recently, not sure why. Here are the pics of the mono, the 2 close up pics wont enlarge for whatever reason.

  https://files.shroomery.org/files/20-21/984232092-20200518_154331.jpg https://files.shroomery.org/files/20-21/984232039-20200518_154324.jpg
I've had trich in 1 mono, and 3 shoeboxes so far, and currently I only have 2 shoeboxes that look fairly normal, out of 11.
I don't think my agar was clean enough for grain. If it was, I think maybe my first RTV lids were too thin and caused leaks. Possibly I spawned them into tubs too early, there was a few grains on some of the tops of the jars that weren't done yet, they should have been left longer. Possibly my inoculation process in the SAB needs some work. I also didn't put foil over my media bottles, I only loosened the caps slightly to let steam in/out. I didn't put foil over my grain jars either, I read somewhere that it wasn't necessary not sure if this is true or if I made this part up.

  
--------------UPDATE-------------- 5-21
These babies are getting large.

  The different color caps is neat.
Here is the other b+ tub, no pins yet.

Here is the shoebox with the pins

Other shoebox that doesnt have pins yet, spawned 5-3 or 4
  
I also have yet to take any pics of this, can someone tell me if its bacteria or what? It is thick dense white growth that is getting bigger and then metabolites or orange/rust/yellow/tan/brown colored liquid fills the centers.
 
 
My pesa tub doesnt have them yet, but nearly all the other tubs have this.

TOC tub looks decent, no pins, spawned 5-1 This one is cased so I can't see if the sides have this.

--------------UPDATE-------------- 5-22
They're trying to escape!
 the 2 are right up against the mp tape lol

shoe box:
  
--------------UPDATE-------------- 5-23
Probably gonna pick these soon.
These pics were from last night

 
These are from today
 
 
 

I don't think any of these tubs are going to produce anything else but these 3 little clusters. The 2 pesa tubs are still early on, spawned 5-12 so they might do good, who knows.
I poured new plates and I will start from prints again, find better growth, clean it farther, and work out my sterile processes. I should wait about a week after 100% colonization to let the jars consolidate before spawning. I will keep transferring this grows plates on, currently about to do t4.
One last edit, here is what I got

Edited by Quadryder (05/23/20 04:51 PM)
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26595906 - 04/12/20 02:28 PM (3 years, 9 months ago) |
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Oh ya, forgot to mention this is my first time doing all of this. I did a pf tek with a spore syringe to get the b+ print 4 years ago that's all the experience I have so far.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26595925 - 04/12/20 02:34 PM (3 years, 9 months ago) |
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Oh yeah I like what I see. Good luck.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: filthyknees] 1
#26595933 - 04/12/20 02:38 PM (3 years, 9 months ago) |
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Thank you very much for your help!
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26615904 - 04/20/20 05:36 PM (3 years, 9 months ago) |
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updated OP
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26639442 - 04/30/20 04:38 PM (3 years, 8 months ago) |
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bump
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 4 hours, 16 minutes
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26639505 - 04/30/20 05:14 PM (3 years, 8 months ago) |
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Right on 
That toc is a fast and sexy bitch
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Stromrider]
#26639643 - 04/30/20 06:05 PM (3 years, 8 months ago) |
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I’ve been wanting to try this TOC. Nice setup you got going on, I wanna see what happens
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Roger Clemency]
#26639801 - 04/30/20 07:27 PM (3 years, 8 months ago) |
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if you really want a print PM me and I'll do my best.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: filthyknees]
#26639803 - 04/30/20 07:28 PM (3 years, 8 months ago) |
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Justweed
Bobby's short shorts



Registered: 04/01/20
Posts: 555
Last seen: 3 months, 17 days
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: shevanel]
#26639953 - 04/30/20 08:59 PM (3 years, 8 months ago) |
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I gotta start up some TOC! Got a print recently from an old friend Grow looks good! Put the jars closer together to keep some temperature in em, keep air from cooling them off and having to warm themselves up.
--------------------
  Keep 'em high and tight guys....
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PinkStormtrooper
Jet-Puffed


Registered: 04/11/20
Posts: 218
Loc: 10001110101
Last seen: 3 years, 3 months
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Justweed]
#26640563 - 05/01/20 06:00 AM (3 years, 8 months ago) |
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not trying to bag on your style but hold up on throwing out jars so fast lol at least let em finish and give em a nose test some of those looked decent IMO
-------------------- "say, you got a little astroglide on your moustache"
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: PinkStormtrooper]
#26644246 - 05/02/20 05:32 PM (3 years, 8 months ago) |
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Ya its kind of hard to tell with the pics. The growth was totally overtaking any parts of the jar that were un-colonized, and way quicker than the thick "normal" growth. I probably could have kept them to see what would happen, I was afraid of bad spores in my area, still new to this.
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MrMong


Registered: 10/16/13
Posts: 70
Last seen: 3 years, 5 months
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26644348 - 05/02/20 06:19 PM (3 years, 8 months ago) |
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I'm excited for you. What a banging first attempt at bulk. Will be following this
-------------------- Flick and Nip
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: MrMong]
#26646855 - 05/03/20 07:41 PM (3 years, 8 months ago) |
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Thanks for all the tips, really appreciate it. Any more help is totally welcome.
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26664765 - 05/11/20 03:22 PM (3 years, 8 months ago) |
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bump
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 7 minutes, 52 seconds
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26664869 - 05/11/20 04:22 PM (3 years, 8 months ago) |
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: tripdawg420]
#26679492 - 05/18/20 05:54 PM (3 years, 8 months ago) |
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we've got trich. How do my tubs look other than that?
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DigDug
Cultivator

Registered: 05/05/20
Posts: 201
Last seen: 7 months, 6 days
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26679503 - 05/18/20 05:58 PM (3 years, 8 months ago) |
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All the experience you have so far is a PF tek grow?
Way to jump head first into this hobby. Keep working at it and you'll iron out the kinks.
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: DigDug]
#26679655 - 05/18/20 07:07 PM (3 years, 8 months ago) |
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Thank you for the inspiration! Yes that is all the hands on so far, the rest has been reading on this forum. Although I have some worry for how this is going to turn out based on the most recent update, I am still ready to keep learning and moving forward despite any failures.
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26680464 - 05/19/20 06:37 AM (3 years, 8 months ago) |
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You can’t be a true cultivator until you’ve had tubs full of your time and energy get destroyed by trich at least you have other ones going on right now and some are looking spiffy.
I always use foil on things in the PC, especially things with lids not all the way tight. Foil is cheap and quick and you can reuse it many many times if you’re careful. Just keep on rocking though. It’s an ambitious first bulk grow so don’t be turned off by the current happenings.
Check your lids and modify if needed. You really have to sand the surface before RTVing a sfd on and make sure the seal is thick enough for no gaps. Study agar plates very closely, most especially if it’s going to nocc a grain master. I will watch a plate for a couple days, mostly watching that outer “exploratory” ring of myc. Sometimes you’ll see it go chaotic in a certain area.
Do you know for sure which jars went into the tubs with trich? Studying pictures of those jars may tell you something
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Roger Clemency]
#26686045 - 05/21/20 04:23 PM (3 years, 8 months ago) |
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I am totally using foil, I also am re making all my lids with thicker beads. I think I took the culture to grain too soon, it was t1 from germ and I don't even know if the transfer I took from the germ plate was myc. To me it looked the cleanest out of all of the growth I saw, but it was not "dense" at all, or ropey like rhyzomorpic growth typically looks.
My current plan is to streak another few germ plates and see if I can find some better growth to start with, and go from there. I will keep my current transfers going, about to do t4 of these cultures.
I also didn't give my jars time to consolidate before spawning them. Failure is the best way to learn.
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26686158 - 05/21/20 05:19 PM (3 years, 8 months ago) |
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Here are my current transfers Any tips on best spots to transfer based on what you see are welcome. These are all t3 from germ plate.
B+, I had 2 prints
 
  
TOC
 

PESA

I know they aren't the best pics, If you wanna see one close up I can make it happen.
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26690818 - 05/23/20 04:54 PM (3 years, 8 months ago) |
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bump cuz the grow is over.
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26690957 - 05/23/20 06:30 PM (3 years, 8 months ago) |
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Nice dude keep at it
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: filthyknees]
#26692523 - 05/24/20 12:03 PM (3 years, 8 months ago) |
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hell ya, you know it
down to 2 pesa tubs and 1 shoebox. lol
 t4
 new germ plates
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26734584 - 06/10/20 02:27 PM (3 years, 7 months ago) |
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So these tubs actually kept flushing outside, unfortunately I didn't snap any pics of the big ol bottom pin or the few clusters.
The b+ I spawned 4-27 has this many pins right now
  The cluster spots (clusters of like 1-7 big shrooms) had about 4 flushes now all these pins came up.
A lot of them look open already, so I don't expect much
 

This mass pinning after only a couple cluster spots leads me to believe that my sub was too wet, not to mention the bacteria. I will try doing the sub a lot more dry next time, still working on cleaning up these plates.
Here's the 2 pesa tubs spawned 5-12 today.
  First fruits for these ones.
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26736987 - 06/11/20 02:41 PM (3 years, 7 months ago) |
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B+ tubs spawned 4-27 today The pins are actually growing wtf noice

  I picked the big fucker, cut the base upwards about an inch and ripped the rest, and then ripped off the cap like an animal and put them in the dehydrator.

EDIT: I don't see any spores but a lot of these are open and flaring out, I didn't see any at all under the big guy either but its in the dryer already
Edited by Quadryder (06/11/20 02:50 PM)
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26742559 - 06/13/20 07:38 PM (3 years, 7 months ago) |
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This tub is nuts. I have harvested a few clusters already

 
  
Some have a nipple, some are turned inside out right after pinning, and some look like normal b+. It's so neat, I don't think ive seen any spores. Gonna let these get big, not sure if I will pick the larger ones and wait for the rest to catch up or just let it go.
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26743577 - 06/14/20 08:11 AM (3 years, 7 months ago) |
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Quote:
Quadryder said: This tub is nuts. I have harvested a few clusters already

 
  
Some have a nipple, some are turned inside out right after pinning, and some look like normal b+. It's so neat, I don't think ive seen any spores. Gonna let these get big, not sure if I will pick the larger ones and wait for the rest to catch up or just let it go.
picked some more
 
 
I found they are just super low spore count, I do not think they are sporeless
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Roger Clemency
Smile


Registered: 03/23/20
Posts: 2,005
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Quadryder]
#26744867 - 06/14/20 08:16 PM (3 years, 7 months ago) |
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That thing ended up working out pretty well Has it been outside?
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Quadryder

Registered: 11/09/15
Posts: 156
Loc: Idk it's dark
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Re: b+,toc,pesa print, a2a, a2g, g2g, bucket, mp tape mono [Re: Roger Clemency]
#26745485 - 06/15/20 03:56 AM (3 years, 7 months ago) |
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It has been in my garage next to a window, I think it was heavily bacterial and too wet to start.
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