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Offlinebigsmallcat
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what do friends
    #26594622 - 04/11/20 11:38 PM (3 years, 9 months ago)

So its contaminated but idk what with. thought and opinions? do I trash it?







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OfflineHamHead
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Re: what do friends [Re: bigsmallcat]
    #26594639 - 04/11/20 11:45 PM (3 years, 9 months ago)

Trash what's in the jar, not the jar.

It's still good.


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The Italian researchersโ€™ findings, published by the INTโ€™s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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Offlinebigsmallcat
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Re: what do friends [Re: HamHead]
    #26594647 - 04/11/20 11:49 PM (3 years, 9 months ago)

Quote:

HamHead said:
Trash what's in the jar, not the jar.

It's still good.




lol yea i wasnt gonna trash the jar, but the spawn inside is what I was asking. so no good?:frown:
What could it have been


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OfflineHamHead
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Re: what do friends [Re: bigsmallcat]
    #26594663 - 04/11/20 11:55 PM (3 years, 9 months ago)

Hard telling. So many thongs could hit a jar.

That's right, it says thongs.

You can always let it go and see what it looks like, as a learning experience.


--------------------
The Italian researchersโ€™ findings, published by the INTโ€™s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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Offlinehazyhorse
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Re: what do friends [Re: bigsmallcat]
    #26594667 - 04/11/20 11:56 PM (3 years, 9 months ago)

looks bacterial. maybe too wet or just bad sterile technique


--------------------
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Offlinebigsmallcat
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Re: what do friends [Re: hazyhorse]
    #26594675 - 04/12/20 12:03 AM (3 years, 9 months ago)

yea I guess its hard to say what happened. I Tried my best to be sterile and used a sterno on whole needle. sprayed everything down with 70%. The syringe was already gonna expire if it hadnt already as it was on its 3rd month. Took too long to get my PC and grain brag from my refund lol. oh well now to start over again and get a new needle. thanks for the inputs my peeps.

Any good vendors yall recommend?


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InvisiblePiptheGreAtest
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Re: what do friends [Re: bigsmallcat]
    #26594682 - 04/12/20 12:10 AM (3 years, 9 months ago)

Not suppose mention vendors here. I try to G2G that mf and see what happens. Does look wet but if it was completely bacterial it would stall out might have some resistance mycelium that might survive in new environment. Was the meddle bought from vendor, They should last long than but if you feel incline do so. Best luck.


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Re: what do friends [Re: bigsmallcat]
    #26594690 - 04/12/20 12:13 AM (3 years, 9 months ago)

unless you're hurting for jars throw some coir on top and top fruit it. you'll probably get a flush or two out of it.

make a print or two from what you get and start in on agar


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Offlinebigsmallcat
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Re: what do friends [Re: PiptheGreAtest]
    #26594705 - 04/12/20 12:23 AM (3 years, 9 months ago)

Quote:

PiptheGreAtest said:
Not suppose mention vendors here. I try to G2G that mf and see what happens. Does look wet but if it was completely bacterial it would stall out might have some resistance mycelium that might survive in new environment. Was the meddle bought from vendor, They should last long than but if you feel incline do so. Best luck.




oops sorry about vendors and thanks for the input.


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Offlinebigsmallcat
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Re: what do friends [Re: trubblesome]
    #26594716 - 04/12/20 12:26 AM (3 years, 9 months ago)

Quote:

trubblesome said:
unless you're hurting for jars throw some coir on top and top fruit it. you'll probably get a flush or two out of it.

make a print or two from what you get and start in on agar





Honestly yea!! Im gonna try this out thanks man just cuz i really dont wanna get a new syringe haha. my only worry was the fruits being contamed but it should be fine if I try this right?? I got 2 bricks of coir so why not.


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Offlinehazyhorse
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Re: what do friends [Re: bigsmallcat] * 1
    #26594717 - 04/12/20 12:28 AM (3 years, 9 months ago)

yeah try top fruiting them. might not work but who knows, you could pull some fruits


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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Offlinebigsmallcat
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Re: what do friends [Re: bigsmallcat]
    #26594720 - 04/12/20 12:30 AM (3 years, 9 months ago)

oh yeah for shits I tried destroying whatever contam happened with 3% peroxide, but obviously didnt work .


Edited by bigsmallcat (04/12/20 12:33 AM)


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Offlinebigsmallcat
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Re: what do friends [Re: bigsmallcat]
    #26594721 - 04/12/20 12:31 AM (3 years, 9 months ago)

thats why its wet


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InvisiblePiptheGreAtest
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Re: what do friends [Re: bigsmallcat]
    #26594731 - 04/12/20 12:36 AM (3 years, 9 months ago)

Quote:

bigsmallcat said:
Quote:

trubblesome said:
unless you're hurting for jars throw some coir on top and top fruit it. you'll probably get a flush or two out of it.

make a print or two from what you get and start in on agar





Honestly yea!! Im gonna try this out thanks man just cuz i really dont wanna get a new syringe haha. my only worry was the fruits being contamed but it should be fine if I try this right?? I got 2 bricks of coir so why not.



G2G to them, if itโ€™s bacterial they wonโ€™t colonize but if so you could fruit a mono if their good (or whereever your trying to spawn too.)


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Offlinebigsmallcat
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Re: what do friends [Re: PiptheGreAtest]
    #26594745 - 04/12/20 12:46 AM (3 years, 9 months ago)





G2G to them, if itโ€™s bacterial they wonโ€™t colonize but if so you could fruit a mono if their good (or whereever your trying to spawn too.)





AHHHHHH ok. i might do it then you've convinced me a little.


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InvisiblePiptheGreAtest
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Re: what do friends [Re: bigsmallcat]
    #26594789 - 04/12/20 01:11 AM (3 years, 9 months ago)

Quote:

bigsmallcat said:




G2G to them, if itโ€™s bacterial they wonโ€™t colonize but if so you could fruit a mono if their good (or whereever your trying to spawn too.)





AHHHHHH ok. i might do it then you've convinced me a little.</font></font>




Effort shouldn't be wasted on mere chance but effort, your action will provide results or experience either one puts you one up.


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Offlinehazyhorse
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Re: what do friends [Re: PiptheGreAtest]
    #26596077 - 04/12/20 04:07 PM (3 years, 9 months ago)

oh, you could also just give it a shake test. shake it up to break up all the grain. if itโ€™s healthy it should recover within a few days


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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OfflineMachiavelliavore
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Re: what do friends [Re: hazyhorse]
    #26596116 - 04/12/20 04:28 PM (3 years, 9 months ago)

I think I see a sporulating mold in the top center of the jar in the first photo.  G2G'ing will make your contamination problems worse.  I think you could get a clean culture out of those using agar quite easily though.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinebigsmallcat
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Re: what do friends [Re: Machiavelliavore]
    #26596503 - 04/12/20 07:30 PM (3 years, 9 months ago)

Quote:

Machiavelliavore said:
I think I see a sporulating mold in the top center of the jar in the first photo.  G2G'ing will make your contamination problems worse.  I think you could get a clean culture out of those using agar quite easily though.




Yes my thoughts exactly. why waste the grains I have on a gamble.Im just gonna try to fruit outta the jar. maybe shake it up a bit and see if I can get a print since ive exhuasted my funds already.


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OfflineMachiavelliavore
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Re: what do friends [Re: bigsmallcat]
    #26597330 - 04/13/20 07:00 AM (3 years, 9 months ago)

Just choose a few grains that look like they have healthy growth, grab them with flamed tweezers, and drop them on agar.  Should get a clean culture within a few xfers.  I wouldn't shake it if you're gonna try and fruit it, you're just distributing contams more.  I'd be kinda surprised if you get anything either way.  Are you out of spores?


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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