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Amazon Shop for: ½ Pint Jars, Agar, Potato Dextrose

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Monkee
Registered: 12/27/98
Posts: 301
Loc: UK
Agar question..
    #2659413 - 05/10/04 02:11 PM (12 years, 6 months ago)

Does it usually take a while before growth starts on agar? I put a few colonised grains of millet on PDA + peroxide about 4 or 5 days ago and there's still no growth out onto the agar.. the grains are all fluffed up with growth but that's it. I was expecting it to start as fast as if the grain had been used in grain2grain jars..


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Invisiblepsilomonkey
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Registered: 08/08/03
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Re: Agar question.. [Re: Sorted]
    #2659769 - 05/10/04 03:58 PM (12 years, 6 months ago)

I would have thought you would be seeing growth on the plate by now.

Are they in a incubator? and how much h2o2 did you use? Also too rich a medium can slow growth.


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OnlineGr0wer
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Re: Agar question.. [Re: psilomonkey]
    #2659837 - 05/10/04 04:41 PM (12 years, 6 months ago)

Give it a few more days for the growth to get used to the peroxide.


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Monkee
Registered: 12/27/98
Posts: 301
Loc: UK
Re: Agar question.. [Re: psilomonkey]
    #2661758 - 05/11/04 03:51 AM (12 years, 6 months ago)

There could well have been a little too much peroxide.. as I understand it store bought stuff can vary in strength from what it says on the bottle.
Looks like a bit of growth is happening on one of the plates now.. am I right in thinking when I use the colonised plate to start more off with it should grow better due to already being used to the peroxide?


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Amazon Shop for: ½ Pint Jars, Agar, Potato Dextrose

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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