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OfflineJustweed
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Pasteurization temp/time?
    #26593618 - 04/11/20 02:13 PM (3 years, 9 months ago)

So for pasteurization I usually get up to 175*F in an oven set to 350*F, then turn off the oven leaving the substrate material in the oven until it cools over several hours. Usually after turning off the oven the sub gets up to about 190*F, then slowly cools. I aim for the material to stay above 175* for at least 1 hour. Any comments on this? Should I aim to cool faster? Lower temperatures? I'm getting to the point where I'm making 2 substrates on the same day, so I'm wondering if I need to bother trying to keep the temperature 175*+ for an hour, or if I can drop it down after it hits 175* and not worry about it. Any input appreciated :smile:


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OfflineMachiavelliavore
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Re: Pasteurization temp/time? [Re: Justweed]
    #26593630 - 04/11/20 02:18 PM (3 years, 9 months ago)

You don't want to break 180F for pasteurization afaik, and many people prefer not to break about 165-170F.  I think straw may be pickier than poo.  Coir doesn't need to be pasteurized and with say peat casing breaking 180F prolly won't cause you problems.  Basically if the substrate has nutrition, you need to be more precise.

https://www.shroomery.org/forums/showflat.php/Number/20822448


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
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OfflineJustweed
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Re: Pasteurization temp/time? [Re: Machiavelliavore]
    #26593635 - 04/11/20 02:20 PM (3 years, 9 months ago)

Quote:

Machiavelliavore said:
You don't want to break 180F for pasteurization afaik, and many people prefer not to break about 165-170F.  I think straw may be pickier than poo.  Coir doesn't need to be pasteurized and with say peat casing breaking 180F prolly won't cause you problems.  Basically if the substrate has nutrition, you need to be more precise.

https://www.shroomery.org/forums/showflat.php/Number/20822448





Yea I probably should have mentioned, I'm using coco coir mixed with bagged composted manure from the hardware store. Pretty simple mix with decent results. These are my first times watching the temperature on pasteurization, I used to just time it and hope for the best, so I'm looking for a dialed in result as far as time+temp goes.


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InvisiblebodhisattaMDiscordReddit
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Re: Pasteurization temp/time? [Re: Justweed]
    #26593641 - 04/11/20 02:23 PM (3 years, 9 months ago)

You can use all that stuff right out of the bag no pasteurizing. :shrug: coir can be hydrated with tap water, pasteurized, or boiled, etc.. doesn't matter


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OfflineMachiavelliavore
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Re: Pasteurization temp/time? [Re: Justweed]
    #26593651 - 04/11/20 02:30 PM (3 years, 9 months ago)

Also keep in mind if your oven is well above 180F the edges of your sub are gonna be getting hotter than the center, so you really don't want your heat source breaking pasteurization temperature ranges or your edges will hit and break pasteurization range before or during the time that your center hits ideal range.

Wouldn't have thought you could use composted poo without propper pasteurization but I don't know anything about the poo lifecycle.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisiblebodhisattaMDiscordReddit
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Re: Pasteurization temp/time? [Re: Machiavelliavore]
    #26593658 - 04/11/20 02:33 PM (3 years, 9 months ago)

The bagged shit from the store can be used as is. There's plenty of people doing it and a few threads too


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OfflineJustweed
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Re: Pasteurization temp/time? [Re: Machiavelliavore]
    #26593731 - 04/11/20 03:26 PM (3 years, 9 months ago)

Quote:

bodhisatta said:
The bagged shit from the store can be used as is. There's plenty of people doing it and a few threads too



Probably could, but I prefer to pasteurize, as I mix in the coir I'm able to remove the rocks and bits of wood that go in it, get it hydrated perfectly, and pasteurizing it gives me a sense of ease about spawning to it. My tubs have been colonizing within 7-10 days, so it doesn't seem to harm them, even if my temps are going a bit too high!

Quote:

Machiavelliavore said:
You don't want to break 180F for pasteurization afaik, and many people prefer not to break about 165-170F.  I think straw may be pickier than poo.  Coir doesn't need to be pasteurized and with say peat casing breaking 180F prolly won't cause you problems.  Basically if the substrate has nutrition, you need to be more precise.

https://www.shroomery.org/forums/showflat.php/Number/20822448



I'm going to start using a lower temperature in my oven and just aim to get the center to 160ish. Any idea on how long to aim to keep temperature in that range for pasteurization? I can bring the temperature down fairly rapidly right now since it's still cold in my area, I can just set it outside with the lid still on and it'll be room temp in an hour or so.


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OfflineMachiavelliavore
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Re: Pasteurization temp/time? [Re: Justweed]
    #26593844 - 04/11/20 04:52 PM (3 years, 9 months ago)

It's not how fast you can bring it down but how high you get it.  There are some bacteria that are thought to be protective of your substrate that die over 180F, while that temperature kills mold spores and maybe some bad bacteria.  If you take it too high you kill the good stuff. 

Basically you want to check your edges to make sure they aren't breaking 180F and make sure your core hits 160-ish.  Easiest way not to worry about overheating your edges is to keep your oven or water under 180F.  I'd play with your oven control and check what kinda temp ur getting so you can find the sweet spot on your dial to get it under 180F.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinerumfor69
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Re: Pasteurization temp/time? [Re: Machiavelliavore]
    #26594042 - 04/11/20 06:48 PM (3 years, 9 months ago)

Those are all sterilizing temps you guys are saying if they're held
for a length of time. Once you start going over the 145°F for and extended
time you start to sterilize. Not that it matters I sterilize my coir
every time.


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OfflineJustweed
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Re: Pasteurization temp/time? [Re: Machiavelliavore]
    #26594145 - 04/11/20 07:46 PM (3 years, 9 months ago)

Quote:

Machiavelliavore said:
It's not how fast you can bring it down but how high you get it.  There are some bacteria that are thought to be protective of your substrate that die over 180F, while that temperature kills mold spores and maybe some bad bacteria.  If you take it too high you kill the good stuff. 

Basically you want to check your edges to make sure they aren't breaking 180F and make sure your core hits 160-ish.  Easiest way not to worry about overheating your edges is to keep your oven or water under 180F.  I'd play with your oven control and check what kinda temp ur getting so you can find the sweet spot on your dial to get it under 180F.



Length of time is a factor in pasteurization, that's why I'm wondering about how long I should keep it in that range at a minimum. I know that an hour is good because that's what I've been using, I'm just wondering if it's necessary or if I can do 10 minutes or less even at that temperature and be fine.


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Invisiblenosf3r4tu

Registered: 03/26/19
Posts: 775
Re: Pasteurization temp/time? [Re: Justweed]
    #26595220 - 04/12/20 08:20 AM (3 years, 9 months ago)

Pasteurization 131 to 140 F for 2 hours for compost.


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