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OfflineInsultingLizard
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Registered: 01/04/20
Posts: 546
Last seen: 3 months, 24 days
Re: Couple questions about cultures [Re: InsultingLizard]
    #26597025 - 04/13/20 12:35 AM (3 years, 9 months ago)

Well, the syringes are definitely contaminated. I made (A) four dishes with one of those (two by directly squirting on the dish and two by streaking with a loop), and (B) two dishes with one of the vendor syringes I still had. There's nothing visible on the surface of the B dishes, but the A dishes are already showing a sort of "raised" texture everywhere the water touched. I'm sure in a few days they'll look like the dish from the OP.

I also transferred from the clone dish and it's looking nice and rhizomorphic:


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Couple questions about cultures [Re: InsultingLizard]
    #26598400 - 04/13/20 04:27 PM (3 years, 9 months ago)

Quote:

InsultingLizard said:
Well, the syringes are definitely contaminated. I made (A) four dishes with one of those (two by directly squirting on the dish and two by streaking with a loop), and (B) two dishes with one of the vendor syringes I still had. There's nothing visible on the surface of the B dishes, but the A dishes are already showing a sort of "raised" texture everywhere the water touched. I'm sure in a few days they'll look like the dish from the OP.

I also transferred from the clone dish and it's looking nice and rhizomorphic:





An now you know for sure, I love agar for this reason. When I make new syringes I always scope them out and test on agar before knocking up anything significant.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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OfflineInsultingLizard
Stranger

Registered: 01/04/20
Posts: 546
Last seen: 3 months, 24 days
Re: Couple questions about cultures [Re: mushhead]
    #26598428 - 04/13/20 04:38 PM (3 years, 9 months ago)

Oh, for sure. This is why I started doing agar in the first place. It's a bit of an up-front cost, but worth it to eliminate some of the guesswork.


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Couple questions about cultures [Re: InsultingLizard]
    #26598439 - 04/13/20 04:46 PM (3 years, 9 months ago)

Premix MEA is only about $15 tops, petridishes you can get in bulk for an amazing price, or you could invest in Jelly jars which eliminates the need to replace plastic petri dishes. Build a flow hood or a simple SAB and away you go. Agar is simple and cheap ^_^


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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OfflineInsultingLizard
Stranger

Registered: 01/04/20
Posts: 546
Last seen: 3 months, 24 days
Re: Couple questions about cultures [Re: InsultingLizard]
    #26598531 - 04/13/20 05:26 PM (3 years, 9 months ago)

Well, a lot of that stuff I can't find here and I can't import it, so...
For now I'm making do with cat food agar and the dishes I have left, which I don't even know when I'll be able to re-stock, since all the lab supplies stores are closed (unless I buy something ridiculous like 500 or 1000 units).

EDIT: On second thought, I might just do that.


Edited by InsultingLizard (04/13/20 05:35 PM)


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Invisiblemushhead
Potato Devourer
I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: Couple questions about cultures [Re: InsultingLizard]
    #26600016 - 04/14/20 10:08 AM (3 years, 9 months ago)

Quote:

InsultingLizard said:
Well, a lot of that stuff I can't find here and I can't import it, so...
For now I'm making do with cat food agar and the dishes I have left, which I don't even know when I'll be able to re-stock, since all the lab supplies stores are closed (unless I buy something ridiculous like 500 or 1000 units).

EDIT: On second thought, I might just do that.




You should always get bulk, it'll save you time and cash in the long run. Also any kind of small mason jar, jelly jar, half pint jelly jars even work well with agar and can be sterilized like any substrate jar.


--------------------
Meditation Principles

Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room


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OfflineInsultingLizard
Stranger

Registered: 01/04/20
Posts: 546
Last seen: 3 months, 24 days
Re: Couple questions about cultures [Re: mushhead]
    #26623031 - 04/23/20 05:53 PM (3 years, 9 months ago)

A little update: I managed to get clean spores by modifying my jars I use for printing. Here's what they look like now:

The wire in the center is a paperclip to hold the cap over the glass without touching it; I stab it so it will come out through the stump of the stipe, and then I bend the wire so the cap won't slide off. The holes allow evaporation, and on top there's some micropore tape to keep things clean and to hold the paperclip in place.

All the spores I got from a jar like this germinated cleanly.


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