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Offlinedoc34
Fungitarian
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Registered: 02/14/04
Posts: 2,667
Loc: Myceliaville !!!
Last seen: 7 months, 28 days
Re: Lets make them more potent! [Re: Transplant]
    #2662031 - 05/11/04 05:29 AM (19 years, 8 months ago)

the above post is all I found after searching for your request, I hope that helped, :cool:


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Offlinedoc34
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Registered: 02/14/04
Posts: 2,667
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Re: Lets make them more potent! [Re: FallenShroom]
    #2662032 - 05/11/04 05:30 AM (19 years, 8 months ago)

thanks! :cool:


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Offlinedoc34
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Re: Lets make them more potent! [Re: doc34]
    #2662192 - 05/11/04 07:40 AM (19 years, 8 months ago)

Outbreaks of mold and bacterial pathogens during mushroom cultivation are responsible for large losses in potential yields. Typical control methods based on organohalogen pesticides (e.g. prochloraz) must be reevaluated due to new environmental norms strictly regulating pesticide residues and due to growing consumer demands for food products free of pesticide residues. The goal of this project is to develop a novel environmentally sound pathogen control strategy based on the higher tolerance of cultivated basidiomycete mushroom species towards oxidative stress compared to pathogen species. The project will evaluate the tolerance of three commercially important mushroom species (Agaricus bisporus, Pleurotus ostreatus and Lentinula edoles) to reactive oxygen (superoxide, hydrogen peroxide and hydroxy radicals) and compare their tolerance that of typical pathogen species (Trichoderma spp. Verticillium fungicola var. fungicola and Pseudomonas tolaaasii). The ligninolytic enzymes naturally produces by mushroom mycelium will be used to increase the oxidative stress by adding substrates which generate reactive oxygen. The production of the lingiolytic enzymes (laccase, magnese peroxidase and aryl alcohol oxidase) during each stage of mushroom cultivation will be inventoried and mushroom strains with high ligninolytic enzyme activities will be selected. The oxidative stress enhancing additives for mushroom strains producing laccase and manganese peroxidase include organic redow mediators or trace metals (manganese); respectively, in combination with simple organic acids. The oxidative stress enhancing additives for mushroom strains producing acryl alcohol oxidase are various benzylic compounds. The effect of these substrates on the type and concentration of the reactive oxygen species produced will be measured in vivo and in vitro. Redox mediators will be designed that are useful at catalytica concentrations, or biodegradable and are non-toxic after service. A secondary benefit expected from the enhances oxidative stress is improved substrate utilization by mushrooms due to increased lignin degradation. The feasibility of the optimized technology in generation reactive oxygen, lowering incidences of pathogen outbreaks and increasing mushrooms yields will be verified during the cultivation of mushrooms under practical conditions. This research will result in the development of a low cost environmentally sound pathogen control method to be used during mushroom cultivation without any accumulation of harmful chemicals. The users of the developed technology will include mushroom cultivators, spawn and substrate suppliers, biological crop protection compagnies, mushroom cultivation education institutes and white rot fungal bioremediation compagnies.


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OfflineTransplant
Janitor
Registered: 05/07/04
Posts: 77
Loc: Texas
Last seen: 19 years, 5 months
Re: Lets make them more potent! [Re: doc34]
    #2662319 - 05/11/04 08:45 AM (19 years, 8 months ago)

Has anyone tried casing with reed canary grass,Phalaris arundinacae? It is so easy to grow and grows wild in so many places and since it does have DMT, maybe have alkaloids precursors.


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Will Screw For Shrooms


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Offlinedoc34
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Registered: 02/14/04
Posts: 2,667
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Re: Lets make them more potent! [Re: Transplant]
    #2662338 - 05/11/04 08:51 AM (19 years, 8 months ago)

Where can this be found?

"reed canary grass,Phalaris arundinacae"


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OfflineTransplant
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Registered: 05/07/04
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Loc: Texas
Last seen: 19 years, 5 months
Re: Lets make them more potent! [Re: doc34]
    #2662353 - 05/11/04 08:56 AM (19 years, 8 months ago)

http://plants.usda.gov/cgi_bin/plant_profile.cgi?symbol=PHAR3

There should also be a link to state distribution, although Texas is not listed, I know it is grown in wetland reserves around Texas based on a rice university study. Do a search on your area and the key words.


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Will Screw For Shrooms


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Offlinedoc34
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Registered: 02/14/04
Posts: 2,667
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Re: Lets make them more potent! [Re: Transplant]
    #2662626 - 05/11/04 10:16 AM (19 years, 8 months ago)



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Anonymous

Re: Lets make them more potent! [Re: ]
    #2662897 - 05/11/04 11:34 AM (19 years, 8 months ago)

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OfflineTransplant
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Registered: 05/07/04
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Last seen: 19 years, 5 months
Re: Lets make them more potent! [Re: doc34]
    #2662974 - 05/11/04 11:54 AM (19 years, 8 months ago)

first link


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Will Screw For Shrooms


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Offlinedoc34
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Re: Lets make them more potent! [Re: ]
    #2663202 - 05/11/04 12:44 PM (19 years, 8 months ago)

When I named this post,I did so for a reason.If a mushroom can be "altered" to be resistant to contams-it can also be,higher yeilding,bigger,better in appearance,taste better,and yes of course it can be made more potent,although thats not why I posted this(but it did get your attention sir).

I could change it if you like?

And dont thank me,I dont deserve it-I am just a mear peon amongst many!
Thank you! :cool:

:cool: :thumbup:


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Offlinedoc34
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Registered: 02/14/04
Posts: 2,667
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Re: Lets make them more potent! [Re: Transplant]
    #2663592 - 05/11/04 02:09 PM (19 years, 8 months ago)

Cant find a distributer for the seeds,only young plants.


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OfflineRandolph_Carter
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Re: Lets make them more potent! [Re: ]
    #2663745 - 05/11/04 02:52 PM (19 years, 8 months ago)

On a large, ecological scale, i'd have to agree with you.
But for the micro-cultivator (such as myself) having a very contamination resistant strain would be a godsend. Given the current environment, and my massive slaughter of all airborne pathogens between crops, i doubt that anything would survive long enough to make a more potent form. It would give the fungus the headstart against the current stage of invaders, and thru proper control of atmospheric and other contaminants, would stay that way for some time.

I'm just interested in the usage for prevention of accidental contaimination (roommate, cat, air vents, etc.)


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"..all those molecules thrashing their kinky little tails, hot for destiny and the street."  Gibson


Nuke baby seals for Jesus!

(This has been a +1 production.)


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Invisiblepsyphon
mneumatic device

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Registered: 11/27/01
Posts: 565
Re: Lets make them more potent! [Re: doc34]
    #2666228 - 05/11/04 11:41 PM (19 years, 8 months ago)

One of the shroomery sponsors carries phalaris seeds. www.iamshaman.com

I've heard things about the DMT degrading when it dries though, does anyone have any info on this?


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"The real voyage of discovery consists not in seeking new landscapes but in having new eyes."
- Marcel Proust

I wish you all ceaselessly flowing moments of happiness.


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Anonymous

Re: Lets make them more potent! [Re: doc34]
    #2666313 - 05/11/04 11:53 PM (19 years, 8 months ago)

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Invisiblewhiterasta
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Registered: 04/09/02
Posts: 1,780
Loc: Oregon
Re: Lets make them more potent! [Re: ]
    #2667740 - 05/12/04 07:18 AM (19 years, 8 months ago)

I have an outdoor bed of Azures growing on Phalaris and alder right now.We shall see what if any effect the alkaloids(pretty dilute) in phalaris have any effect on potency. As a straw it colonizes quite rapidly.
WR


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OfflineGwaihir
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Registered: 05/05/04
Posts: 4
Last seen: 18 years, 6 months
Re: Lets make them more potent! [Re: doc34]
    #2673333 - 05/13/04 11:46 AM (19 years, 8 months ago)

Used to visit very frequently years back. Lost login. . I must say this is a very interesting post. Wish my mind could absorb 100% of that info, aa so littel time to learn all we want. Interesting nonetheless.

Just tell me what to do to help make them more potent. hopefully a position with the word Guiney in it


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Anonymous

Re: Lets make them more potent! [Re: psyphon]
    #2675095 - 05/13/04 06:23 PM (19 years, 8 months ago)

The problem with adding anything to the substrate for increasing potency is that the mushroom tends to break it up and use it for primary growth. It uses it as food first then what ever it has left over after growth slows and storage increases, it uses for secondary metabolism.

I would think the timing of the application would be critical also.


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OfflineGr0wer
always improving
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Registered: 09/16/03
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Trusted Cultivator
Re: Lets make them more potent! [Re: doc34]
    #2675636 - 05/13/04 08:14 PM (19 years, 8 months ago)

why not just grow high potency shrooms like pesa, penis envy, and p. cyan.


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Offlinesuboriginal
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Registered: 05/06/04
Posts: 278
Last seen: 19 years, 3 months
Re: Lets make them more potent! [Re: doc34]
    #2676714 - 05/14/04 02:54 AM (19 years, 8 months ago)

Might the 'random mutation' / 'accelarated evolution' method be applicable to craeting new variants of shroom ? It's a bit of a hit and miss 'alchemist' type approach, but it would be easy and cheap to try... put simply, you'd grow up a heterogenous bunch of mycelia, then zap it with varying degrees of gamma radiation... then plate out different samples from the zapped concoction... many will be mutated genetic variants. Refine each 'substrain' to a 'monoculture' (pure strain). Then assess these substrains for the desired traights, for example, speed of growth, or levels or certain chemicals. Pick the best ones, or the substrain that has the most desirable mix of traits, and, you guessed it, grow up a big batch and zap it... repeat above process until you have created the super shroom from wonderland... chuckle... compared with, say, plants, shrooms are much more amenable to this approach, cause you can subculture quickly...
Just a thought, would be interested on anyone's opinion...


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Peace, love and organic brown rice...


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Offlinesuboriginal
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Registered: 05/06/04
Posts: 278
Last seen: 19 years, 3 months
Re: Lets make them more potent! [Re: suboriginal]
    #2676739 - 05/14/04 03:13 AM (19 years, 8 months ago)

'General' pathogen resistance would actually be very hard to achieve in my humble opinion, as shrooms are so susceptible to a myriad of infections, moulds, other fungi, bacteria, etc... usually the aim is to make a crop resistant or more resistant to a single pathogen that devastates yields of that particular crop, but inducing 'broad spectrum resistance to everything' seems fanciful. One conceivable way would be to insert multiple antibiotic genes using the agarobacterium as is (beautifully!) described above, so that the shroom itself releases antibiotics into the media/ substrate... but even then as also mentioned above, there will always be variant pathogens around waiting for their 'niche' to crop up... Also, might not be good to eat a bunch of antibiotics with your shroom, I guess there's a possibility of allergic reactions and knocking of all your 'natural flora' ('good' bacteria) from your intestine, actually leaving you more susceptible to colonisation by pathogens... Maybe the most realistic way to enhance pathogen resisttance is indirectly, by generating a superfast growing variety... then it will at least have a better chance of outcompeting pathogens...
Again just my humble little ponderings, would be interested in anyone's thoughts...


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Peace, love and organic brown rice...


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