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OfflineDomno
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Why bother with syringes
    #26590450 - 04/10/20 08:06 AM (3 years, 10 months ago)

This is a question based on my own experience so far. To date I have had one print and 6 syringes delivered to my home. The print is doing fine and I am on track to either go g2g or start a shoebox soon. 

The syringes on the other hand were horrible.  To be clear, 5 out of the 6 were not from a trusted vendor, however the one from the vendor was unusable either. 

I tried tiger drop, syringe to loop to agar, syringe to shotglass to loop to agar.  I even tried putting some spores in an LC jar to see what would happen.  Mold, bacteria, garbage.  I have had literally no myc growth from a syringe at all.

Then I decided to mess around with making my own syringes.  Took my syringes, filled them up, wrapped in foil and PC'd with some agar dishes.  Made a syringe using some of my shiny new print.  More contamination. 

Meanwhile, almost every agar plate I started from the print, was clean and strong right from the beginning.  While it was a few bucks more it came with enough spores to make several syringes.  I understand that syringes are cheaper (per unit, not for the quantity of spores), and I have noticed that occasionally vendors will be out of prints of something while having syringes in stock. So unless you are looking for something hard to find, why not just spend a few more bucks and get a print any way?


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Invisiblegizmo1
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Re: Why bother with syringes [Re: Domno] * 1
    #26590461 - 04/10/20 08:16 AM (3 years, 10 months ago)

You tiger dropped a syringe?
While I agree that prints are better, I'm thinking there was some user error here. You said you got clean growth from the print but contamination when using a syringe made from the same print? While there can be a area of a print that is dirty while the rest is clean I don't think that is what happened I'm thinking maybe you introduced contams. Lots of people use syringes and they work for them, but you use 6 syringes and can't get growth from a single one? Something weird is going on here.


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Invisiblewoofwoof
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Registered: 01/04/19
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Re: Why bother with syringes [Re: gizmo1]
    #26590502 - 04/10/20 08:38 AM (3 years, 10 months ago)

Syringes also carry water, which is nasty on agar.
I used a swab from a print too and had not one single contam on my agar, even after 4 transfers. I used to be scared of prints and always did the syringe. But after doing the print>swab>agar I was very impressed and will never go back to syringes again (unless I have to hahaha)


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Invisiblenosf3r4tu

Registered: 03/26/19
Posts: 775
Re: Why bother with syringes [Re: gizmo1]
    #26590508 - 04/10/20 08:41 AM (3 years, 10 months ago)

If you use agar I can't think of a reason why you'd want to make a spore syringe.
For one syringe you use 10-15% of a print. Swiping a loop over a print for agar takes less than 1%.
Contams are visible on plates but not in syringes.
Not to mention colonization times especially if you go from agar to LI.


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OfflineLogicaL ChaosM
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Re: Why bother with syringes [Re: Domno]
    #26590512 - 04/10/20 08:43 AM (3 years, 10 months ago)

Syringes are intended for PF tek and microscope research and thats about it.

Im developing a spore syringe cleaning tek that uses a tiny amount of Lysol spray mixed with the spore water within the syringe. It seems to work. Feel free to try it out yourself :thumbup:


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Invisiblewoofwoof
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Re: Why bother with syringes [Re: LogicaL Chaos]
    #26590515 - 04/10/20 08:44 AM (3 years, 10 months ago)

Quote:

LogicaL Chaos said:
Syringes are intended for PF tek and microscope research and thats about it.

Im developing a spore syringe cleaning tek that uses a tiny amount of Lysol spray. It seems to work. Feel free to try it out yourself :thumbup:




Yea I have been follwoing the thread and does seem interesting.
You are still PCing the grains tho right or steaming the brf cakes??


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OfflineLogicaL ChaosM
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Re: Why bother with syringes [Re: woofwoof]
    #26590519 - 04/10/20 08:46 AM (3 years, 10 months ago)

Im using PF cakes at the moment. I havent tried it with grains yet.

In case youre wondering OP: https://www.shroomery.org/forums/showflat.php/Number/26548586#26548586


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OfflineMachiavelliavore
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Re: Why bother with syringes [Re: LogicaL Chaos] * 2
    #26590609 - 04/10/20 09:30 AM (3 years, 10 months ago)

Syringes are a great starter tool for both cakes and grains and I have had a great deal of success with them.  Starting with agar is kinda intimidating.  I have had great success with my home made syringes, even from open air prints, never purchased from a vendor.  How these vendors are fucking up syringes when they are charging so much is beyond me.  Print an invitro cap in a sterile container and do the transfer in front of a flow hood, and a syringe should have extremely high success rates.  My guess is these vendors pre-prep syringes and let'm sit too long and don't work from invitro prints.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineBig Worm
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Re: Why bother with syringes [Re: Machiavelliavore] * 1
    #26590649 - 04/10/20 09:51 AM (3 years, 10 months ago)

I feel like I never see prints or slants on sites that sell spores. 

How else do people get started growing if they don’t get a syringe to put onto agar first ?

And assuming they don’t know any members to get a print from.


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OfflineMachiavelliavore
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Re: Why bother with syringes [Re: Big Worm] * 1
    #26590674 - 04/10/20 09:58 AM (3 years, 10 months ago)

Many sites sell prints, they just tend to cost like 25$ and usually don't have every variety in print form.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineLogicaL ChaosM
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Re: Why bother with syringes [Re: Big Worm]
    #26590694 - 04/10/20 10:05 AM (3 years, 10 months ago)

Good to see ya Big Worm :yesnod:

Spore prints are somewhat less common than syringes, but they are out there.


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OfflineOregonic
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Re: Why bother with syringes [Re: Big Worm] * 1
    #26590695 - 04/10/20 10:05 AM (3 years, 10 months ago)

Aside from PF Tek you're not wrong, there isn't much reason to use syringes however I will say I've had better luck in terms of old syringes vs. old prints to agar, even when trying to force hydrate the spores from a print. So maybe something could be said for using a water spore solution for long term storage, or maybe I just got lucky :shrug:

That said, I'll probably never buy another syringe again unless it's all a vendor has when looking for something specific.


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OfflineSockadin
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Re: Why bother with syringes [Re: Oregonic]
    #26590821 - 04/10/20 11:04 AM (3 years, 10 months ago)

And no one will sell slants of illegal specimens. Syringes are good for LC


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OfflineDomno
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Re: Why bother with syringes [Re: Sockadin] * 1
    #26590983 - 04/10/20 12:28 PM (3 years, 10 months ago)

I appreciate all of your feed back. I wasn't sure if working with syringes was going to be a make or break skill in this hobby.

Edit: also, I did not tiger drop a syringe. That was the wrong term. I just dropped a drop onto the agar plate.


Edited by Domno (04/10/20 12:40 PM)


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Invisiblegizmo1
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Re: Why bother with syringes [Re: Oregonic] * 1
    #26592172 - 04/10/20 09:17 PM (3 years, 10 months ago)

Quote:

Domno said:
I appreciate all of your feed back. I wasn't sure if working with syringes was going to be a make or break skill in this hobby.

Edit: also, I did not tiger drop a syringe. That was the wrong term. I just dropped a drop onto the agar plate.



Yea I figured that's what you meant lmao.
Quote:

Oregonic said:
Aside from PF Tek you're not wrong, there isn't much reason to use syringes however I will say I've had better luck in terms of old syringes vs. old prints to agar, even when trying to force hydrate the spores from a print. So maybe something could be said for using a water spore solution for long term storage, or maybe I just got lucky :shrug:

That said, I'll probably never buy another syringe again unless it's all a vendor has when looking for something specific.



I've experienced the exact opposite. Can't ever get a old spore syringe to germ but almost always get old prints to.
I may revisit this though I've got a 4 or 5 year old untouched vendor syringe that I was going to toss because I have bad luck with germing old spores from syringes maybe you just inspired me to try it out.


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OfflineThrill
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Re: Why bother with syringes [Re: gizmo1] * 1
    #26592196 - 04/10/20 09:30 PM (3 years, 10 months ago)

I dont get all the hate for syringes. Where are yall buying them where youre getting so many fucked up syringes? The vendor Ive been buying from since I started 10 years ago almost has never sold me a trashy syringe. All of them went into BRF jars or grain spawn. No contams. At least 2 dozen of them.

I like starting with syringes. Gives you a bigger genetic diversity to start with.


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OfflineLogicaL ChaosM
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Re: Why bother with syringes [Re: Thrill]
    #26592206 - 04/10/20 09:37 PM (3 years, 10 months ago)

FINALLY! :lol:

Yeah, most my syringes are quite clean. Sometimes I get one with mold but it could be my technique/SAB/grow space as well.


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OfflineThoughtfulJoker
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Re: Why bother with syringes [Re: Thrill] * 1
    #26592216 - 04/10/20 09:40 PM (3 years, 10 months ago)

I think it really depends on what you are trying to accomplish.

Syringes are useful for starting batches, especially if you don't have a flow hood for transfers, agar work can be a little daunting for some.

I'd say why not master both and use what works best for you?


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OfflineOldManRiver
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Re: Why bother with syringes [Re: Domno]
    #26592262 - 04/10/20 10:00 PM (3 years, 10 months ago)

I've purchased at this point six syringes over the past three years, never had a dud.  Never had significant contamination identifiable to the syringe, never had a blank.  Two of the major vendors, six separate purchases.


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OfflineOldManRiver
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Re: Why bother with syringes [Re: Machiavelliavore]
    #26592277 - 04/10/20 10:06 PM (3 years, 10 months ago)

Quote:

Machiavelliavore said:
Syringes are a great starter tool for both cakes and grains and I have had a great deal of success with them.  Starting with agar is kinda intimidating.  I have had great success with my home made syringes, even from open air prints, never purchased from a vendor.  How these vendors are fucking up syringes when they are charging so much is beyond me.  Print an invitro cap in a sterile container and do the transfer in front of a flow hood, and a syringe should have extremely high success rates.




As far as making them goes, I suspect that most of us don't have a flow hood available. It takes me half an hour to get set up for what you can flick a switch to make happen.  That's an impediment to making quality syringes for the small time amateur.  Not slagging ya, just that explains where some poor ones might come from.

For me, agar is easier than trying to construct a syringe.


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