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OfflineLogicaL ChaosM
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
    #26590536 - 04/10/20 08:53 AM (3 years, 10 months ago)

Perhaps use a spore print for an experimental "spore cleaning" technique?

https://www.shroomery.org/forums/showflat.php/Number/26548586#26548586


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OfflineSt.Pedro
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro] * 1
    #26590568 - 04/10/20 09:06 AM (3 years, 10 months ago)

thank you so much Gizmo!!!
That Agar powder, how about if it got sugar and strawberry taste? Or better banana? :wink:
I do have an oven!! That should do too, for sterilize, right?
That SAB would hardly fit into a pressure cooker, I'd think. In the oven it would, but wouldn't is melt away, even if PP5? Or is it enough, for the SAB, to clean with  alcohol, after all? Wouldn't want to loose my sanity anyway.. :wink:

thank you very much for your kind answers Machiavelli and Logical Chaos! Shall look into your suggestions and may come back later with related questions, if such appear


Edited by St.Pedro (04/10/20 09:11 AM)


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OfflineMachiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
    #26590629 - 04/10/20 09:42 AM (3 years, 10 months ago)

I have sterilized agar in a microwave and it can be done in a boiling water bath as well.  It's just kind of a pain in the ass, which is why there aren't really teks for it, it takes a lot of testing.  You only need a small pressure cooker for agar, you can probably find one on ebay for like $25 or something, like a 6qt.

PP5 (polypropylene) won't melt at 15psi (252? deg F)

Still air box doesn't need to be sterilized, just wipe it down with 1:10 bleach or 70% Isopropyl every once in a while, and spray soapy water for use.  You're just trapping contaminants in liquid and letting anything in the air settle.
https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888

Bod has a lot of good info on agar, check his shit out.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinepoisoned
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
    #26590894 - 04/10/20 11:45 AM (3 years, 10 months ago)

You don't need to sterilize the SAB. Just wash it with soap and use it.

I'd highly recommend you to get pure agar if available. Otherwise, you could probably get that flavored stuff to work.


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OfflineSt.Pedro
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Re: old spore prints, best way to utilize them ? [Re: poisoned]
    #26592635 - 04/11/20 03:14 AM (3 years, 10 months ago)

had time for some shopping today, looking quite good, right?



These glass containers with aluminum lids should serve well, and here, in local shop, cost me close to nothing



now time I study up more on how to prepare the agar agar.
didn't really get yet how to add nutrients.

Bod, this is Bodhisatta, right? Just found an interesting thread of his..


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Offlinepoisoned
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
    #26592687 - 04/11/20 04:08 AM (3 years, 10 months ago)

Bod = bodhisatta, yes.

There's many ways to prep agar. I like to cook it before PC. Some people just mix the ingredients and put into the PC. I personally also find most recipes a bit strong in both agar and nutrients. 7/7/500 agar/MEA/water works better for me.


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OfflineMachiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: poisoned]
    #26593234 - 04/11/20 11:01 AM (3 years, 10 months ago)

Agar looks like regular agar powder so good there.  Those mug-type mason jars may not hold up to the heat, as they are prolly cheap glass not pyrex.  Be gentle with them in terms of temperature changes, like put them in a warm water bath before steam sterilizing/pc'ing or they may crack.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Invisiblegizmo1
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
    #26593358 - 04/11/20 11:58 AM (3 years, 10 months ago)

Quote:

poisoned said:
Bod = bodhisatta, yes.

There's many ways to prep agar. I like to cook it before PC. Some people just mix the ingredients and put into the PC. I personally also find most recipes a bit strong in both agar and nutrients. 7/7/500 agar/MEA/water works better for me.



If doing no pours you can't just mix and pressure cook you have to heat it and dissolve before pouring into the containers. I agree with the agar recipes being a little on the high side I like to use 8 grams agar and 8 grams malt extract to 500ml I don't notice much difference if any in growth when using less over using 10/10/500 but using less I'm preserving resources if nothing else. I don't really like use less than 8 grams agar to 500ml water because I don't like agar that's on the softer side but I've bee. Thinking about trying sagari. :shrug:

Quote:

St.Pedro said:
thank you so much Gizmo!!!
That Agar powder, how about if it got sugar and strawberry taste? Or better banana? :wink:
I do have an oven!! That should do too, for sterilize, right?
That SAB would hardly fit into a pressure cooker, I'd think. In the oven it would, but wouldn't is melt away, even if PP5? Or is it enough, for the SAB, to clean with  alcohol, after all? Wouldn't want to loose my sanity anyway.. :wink:

thank you very much for your kind answers Machiavelli and Logical Chaos! Shall look into your suggestions and may come back later with related questions, if such appear



Lmao no. You won't be sterilizing the SAB I don't even wipe mine with alcohol beforehand. Just loaf it with what I'm working on and wait for air to settle before going to work.

Also I don't see anything to use as nutrients for your agar in that pic do you know what kind of agar you will be making yet? Dry dog food is supposed to work pretty good potatoes water or potato flakes also both should both be available at local stores.
I don't have much experience with either so I can't really give recipes. I've always just used MEA and grain soak water for the most part, but it should be pretty easy to find a good recipe for either of you use the search function.


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Offlinepoisoned
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
    #26593448 - 04/11/20 12:55 PM (3 years, 10 months ago)

Quote:

Machiavelliavore said:
Those mug-type mason jars may not hold up to the heat, as they are prolly cheap glass not pyrex.




While proper mason jars are of better quality (less bubbles and impurities), they are not made out of borosilicate glass.


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How I do glass dishes


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InvisiblebodhisattaMDiscordReddit
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
    #26593546 - 04/11/20 01:35 PM (3 years, 10 months ago)

Quote:

Machiavelliavore said:
Agar looks like regular agar powder so good there.  Those mug-type mason jars may not hold up to the heat, as they are prolly cheap glass not pyrex.  Be gentle with them in terms of temperature changes, like put them in a warm water bath before steam sterilizing/pc'ing or they may crack.



Normal glass won't crack unless its subject to a greater than 99° temperature difference suddenly. Mason jars are cheap glass. The cheapest junk glass there is besides the stuff they make windows for india out of


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OfflineMachiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: bodhisatta]
    #26593567 - 04/11/20 01:44 PM (3 years, 10 months ago)

Wellp, guess mason jars are shit.  It has been my experience that they crack less than re-used jars from store-bought goods, and I would be a bit careful with those.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
OfflineSt.Pedro
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
    #26624149 - 04/24/20 08:32 AM (3 years, 9 months ago)

Sorry good,helpful people I had not come back earlier.
Something unexpected had happened that had kept me busy. Two days ago finally I did inoculate the agar dishes. Very primitively, for lack of materials as e.g. a pressure cooker, in case there still will be success I shall report back, thank you again, cheers!


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OfflineWolfenstein
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
    #26624809 - 04/24/20 01:45 PM (3 years, 9 months ago)

Report back regardless of success. No such thing as a failed experiment :thumbup:


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