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Grungeman17


Registered: 05/06/09
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Has anyone just isolated from ms grain knock ups?
#26589714 - 04/09/20 09:03 PM (3 years, 9 months ago) |
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Exactly, I have outdoors as well as indoor endevors to fulfill. Say I knocked some bags but now ill have flow hood and could perform g2gs. For whatever Im gonna do indoors and consider the priority of the 2, can I just take my top performer. And do a g2g. I know people do this sah if you just havnt got into agar yet and but its comin up for you but you got this goin now and aquired a flowhood in the middle of it. What the name for isolating via grain instead of agar I can easily choose who I think will be the best.
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Machiavelliavore
Vermiculite Hater



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Re: Has anyone just isolated from ms grain knock ups? [Re: Grungeman17]
#26590664 - 04/10/20 09:56 AM (3 years, 9 months ago) |
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It wouldn't be isolation, it would be propagation/cloning of specific multi-spore lineages. Over time they will exchange genetic information and approach effectively being a monoculture. I basically tried to do what you are describing, continuing MS lineages via g2g with no agar. It ended in disaster because you can't always see contamination in grains, it can hide them very well. I did a shitton of g2g's transfering bacteria, and it all went to shit and wasted my time. Just get agar ASAP. Until then I'd just go MS, I had much better luck making my own clean syringes then g2g-ing with no agar.
Good luck.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Grungeman17


Registered: 05/06/09
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Re: Has anyone just isolated from ms grain knock ups? [Re: Machiavelliavore]
#26590840 - 04/10/20 11:09 AM (3 years, 9 months ago) |
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So your saying if I let this bag completely colonize, and then I prep new bags for a transfer from it it has a good chance to contam? Even under the flow hood. Lets say I think this bag right here is it. Like its so goddamn rhizomorphic and colonized so evenly. Others are good and ok i would be happy to just plant those. But i want to take this good one right here and stretch it across 6 bags. And tub em up when they're done. By the time the grow ends ill have some agar going hopefully and will do a fruit body transplant if I want to kep the strain. Where are my current flaws with what i currently got available. I didnt think I had a big contam chance with a flowhood just doing a fully colonized g2g to stretch the one.
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Justweed
Bobby's short shorts



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Re: Has anyone just isolated from ms grain knock ups? [Re: Grungeman17]
#26590878 - 04/10/20 11:35 AM (3 years, 9 months ago) |
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I recently tried making LC isolates/clones. I did MS>Grain, then when I had the grain totally colonized, and some rhizo growth was reaching up from the top layer of grain, I opened the jar and used tweezers to grab a small 'tentacle' of growth. I put that in a jar of honey LC, and let that colonize for about 2 weeks. The LC looks great, clean. The other day I used the LC to noc jars, it's only been 2-3 days, but so far clean, so no cobweb and probably no lipstick! We'll see how that goes.
Something I've done in the past with g2g attempting to isolate is removing 1 grain from one jar and adding it to a fresh jar. This results in agar looking growth, round growth from one kernel spreading through the jar. Obviously this is not 'as good' as agar, and is not a true isolate, but it's an easy way to start, and you could then drop grains from this jar to another to further isolate the 'strain'. I've never gone past doing the 1 kernel into 1 jar, then I've used that whole jar to noc other jars. Works out pretty great.
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  Keep 'em high and tight guys....
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Roger Clemency
Smile


Registered: 03/23/20
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Re: Has anyone just isolated from ms grain knock ups? [Re: Grungeman17]
#26590881 - 04/10/20 11:36 AM (3 years, 9 months ago) |
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Spore prints/ syringes aren’t sterile, since the mushroom they came from was grown in open air (unless someone did a totally sterile grow all the way through and never made mistakes) so if you have a colonized bag of grain that looks good, it’s still possible that there are other organisms in there with it. Could be bacteria or a sly mold.
You can go MS to grain and then G2G but it’s a risk is all. With agar and several transfers you can be pretty much positive that only the mycelium you want is there.
The flow hood in your scenario doesn’t address that the spores came from a mushroom grown in open air. It will allow you to do the G2G safely, but you may be transferring contaminants from your original bag.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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Grungeman17


Registered: 05/06/09
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Re: Has anyone just isolated from ms grain knock ups? [Re: Roger Clemency]
#26591061 - 04/10/20 01:13 PM (3 years, 9 months ago) |
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Thank you for these responses those answer really broadened my understanding of the sitch. Im going to just go straight to tub this time from ms. They came from a repuatable source, and have my agar plates ready for when/if I get something I want. Ive had ALOT of luck with ms. But im ready to be in the iso club. I like the 1 grainer idea. But I do see the risks involved. All my bags pretty much look fantastic and are moving just great. But there is a frontrunner it made me ask these questions. Thank you all.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Re: Has anyone just isolated from ms grain knock ups? [Re: Grungeman17]
#26591365 - 04/10/20 03:38 PM (3 years, 9 months ago) |
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i think the greatest risk is bacteria expanding undetected as you g2g. You can always grab a sample of the spawn, put it in a sterile jar, n stick it in the fridge, drop that on agar later. You may have have good experience with g2g, but IME it seems to exacerbate the problems with syringe grows. I too have had success with many syringe to grain grows.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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bw86
Doesn't play well with others


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Re: Has anyone just isolated from ms grain knock ups? [Re: Grungeman17]
#26591368 - 04/10/20 03:39 PM (3 years, 9 months ago) |
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There is absolutely nothing wrong with using MS to grain then choosing the best jar for g2g. Yall can argue that all you want I really don't care. That is the way it was done for decades... A MS culture CAN out perform a random Isolate you know nothing about.
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