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St.Pedro
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old spore prints, best way to utilize them ? 1
#26588448 - 04/09/20 09:40 AM (3 years, 9 months ago) |
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Hi,
I have a small collection of spore prints which are quite old.. They had been kept in individual plastic bags, some again wrapped into tin foil, and all together in a heavy, dark plastic bag, in a tropical, humid place for some 4 to 5 years. How to I best go about to use them? - I have no prior experience in growing, I'll need very detailed guide, links, tips, thank you!

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Wolfenstein
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro] 1
#26588504 - 04/09/20 10:13 AM (3 years, 9 months ago) |
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Put that search bar to work buddy! It'll seriously work wonders
-------------------- Anything and everything I say, write, and/or imply is purely false and is only done so with the intent to enhance my acting career. Molon Labe As above, so below
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poisoned
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Re: old spore prints, best way to utilize them ? [Re: Wolfenstein] 1
#26588540 - 04/09/20 10:28 AM (3 years, 9 months ago) |
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probably best to start with agar. You'll quickly see if anything is viable and knowing how to do agar will help you a lot with other aspects of growth
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6 Speed
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Re: old spore prints, best way to utilize them ? [Re: Wolfenstein] 1
#26588543 - 04/09/20 10:30 AM (3 years, 9 months ago) |
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You'll want to rehydrate the spores and you can then either make a spore syringe or us the print to inoculate agar.
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gizmo1



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Re: old spore prints, best way to utilize them ? [Re: 6 Speed]
#26588598 - 04/09/20 11:01 AM (3 years, 9 months ago) |
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They will need to go straight to agar then they will either work or they won't. The agar will do all the hydrating. When working with old prints I like to do multiple dishes for each print and scrape plenty of spores into each dish you want alot of spores on there to give a better chance of germination. Then just wait. Old spores will usually take longer to germ like a couple weeks longer. If no germination after the 4th week I usually toss and try again. I would like to add that this is not necessary for a print that is on 3 or 4 years old those usually get treated normally and of they werestored correctly should germinate fine. 5 years and up where I start having problems germing prints ime. Use the search function to figure out how to do no pours if you don't want to buy petris but I'd definitely use agar for older prints.
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St.Pedro
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Re: old spore prints, best way to utilize them ? [Re: gizmo1]
#26588631 - 04/09/20 11:20 AM (3 years, 9 months ago) |
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thank you so much good people for your encouraging answers, specially gizmo! Where I am at I won't be able to get anything prepared, will have to rely on what I get in local pharmacy, drug stores and build myself from scratch Time I do hit that search button, right, on how best to prepare the agar agar solution, petry dish, to inoculate asf. asf. As mentioned it all will be a first for me, any more direct help right here, in this thread, or links, even if I may ( or may not, straight away..) find myself in search, much appreciated!
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poisoned
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26588700 - 04/09/20 11:56 AM (3 years, 9 months ago) |
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you should be able to get resources for agar in a grocery store.
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Smartattack
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Re: old spore prints, best way to utilize them ? [Re: poisoned] 2
#26588933 - 04/09/20 01:57 PM (3 years, 9 months ago) |
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Super soaker plus local cow farm.
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mycoflow
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Re: old spore prints, best way to utilize them ? [Re: Smartattack]
#26589010 - 04/09/20 02:38 PM (3 years, 9 months ago) |
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I wouldn't unwrap them outside of a Still Air Box so you don't introduce contaminants. (re: your last pic)
But yeah, search the forum - all the info is there! Start with Agar and putting these spores to agar. Best way to find out if they are still viable.
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gizmo1



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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26589892 - 04/09/20 11:10 PM (3 years, 9 months ago) |
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Quote:
St.Pedro said: thank you so much good people for your encouraging answers, specially gizmo! Where I am at I won't be able to get anything prepared, will have to rely on what I get in local pharmacy, drug stores and build myself from scratch Time I do hit that search button, right, on how best to prepare the agar agar solution, petry dish, to inoculate asf. asf. As mentioned it all will be a first for me, any more direct help right here, in this thread, or links, even if I may ( or may not, straight away..) find myself in search, much appreciated!
Can you get agar locally? Asian markets usually carry it. Health food stores also but it will be more expensive. You can easily find nutes for the agar at a local store, dog food is one option. You are also going to need a good sized plastic tote to use as a SAB if you dont already have one. You will neef some sort of container to use as a no pour "petri" this can be small mason jars like half or quarter pint jars, glad mini roumds or snall tupperware. If you get small jars make sure they arent quilted and are clear. If you go with plastic make sure it is pp5(check the recycle code on bottom) to make sure it is autoclavable. Do you have a pressure cooker yet? You will need one to sterilize your agar.
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OldManRiver
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Re: old spore prints, best way to utilize them ? [Re: gizmo1]
#26589955 - 04/10/20 12:10 AM (3 years, 9 months ago) |
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Honestly, unless you have some attachment to them, spores are so easy to acquire anymore that that they have not much significance. If you wish to grow, it would be safer to spend $25, and get a recent equivalent of those to start your efforts.
Sorry to be a downer.
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gizmo1



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Re: old spore prints, best way to utilize them ? [Re: OldManRiver] 1
#26589958 - 04/10/20 12:16 AM (3 years, 9 months ago) |
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Whats wrong with trying it first instead of just throwing money down? I mean I've used very very old prints and its always just that much cooler when you germ prints that are from back in 99 lol. He has enough prints and they arent suuuper old so im sure it wont be impossible to get germination. Plus with this coronavirus shit going on stuff can be harder to get as well as take alot longer to show. Might as well give er the old college try fellers.
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Edited by gizmo1 (04/10/20 12:18 AM)
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OldManRiver
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Re: old spore prints, best way to utilize them ? [Re: gizmo1]
#26590037 - 04/10/20 01:55 AM (3 years, 9 months ago) |
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Well, speaking from my own recent experience, PDA plates from Carolina Biological cost me $5 each. I have three cultures going right now from sketchy sources, it will cost me two more plates each to curate what happens. I have one plate of someones old spores going right now nothing is happening. That's 40 bucks in plates for experimentation.
Present value of time and money, my friend. In my day job, I make more than $25/hr, why waste the time?
Unless there is sentimental or mythical strain attachment, shit, spores are cheap/
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gizmo1



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Re: old spore prints, best way to utilize them ? [Re: OldManRiver]
#26590048 - 04/10/20 02:10 AM (3 years, 9 months ago) |
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Quote:
OldManRiver said: Well, speaking from my own recent experience, PDA plates from Carolina Biological cost me $5 each. I have three cultures going right now from sketchy sources, it will cost me two more plates each to curate what happens. I have one plate of someones old spores going right now nothing is happening. That's 40 bucks in plates for experimentation.
Present value of time and money, my friend. In my day job, I make more than $25/hr, why waste the time?
Unless there is sentimental or mythical strain attachment, shit, spores are cheap/
Its not like you have to babysit It while you wait. Scrape the spores on the plate and walk away for a week or two. also thats rediculous I would never pay that price. Or even buy premade plates for that matter I get an entire case of 500 plates for 90 bucks and thats high. I mean I guess I get why you do it. Basically you were saying your tkme is too valuable right? Maybe pay a friend or get your wife to pour some plates for you. That price sounds like rape to me. Especially if you use alot of plates. Maybe in your case it would make sense to buy fresh spore from a reputable source but no serious cultivators are out here buying premade plates at 5 bucks a pop we dont really have to worry about using a extra plate or two. Im not hating or trying to talk down at all just saying.
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Machiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: gizmo1]
#26590152 - 04/10/20 04:03 AM (3 years, 9 months ago) |
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Just germinated spores from prints that were 5-7 years old on agar. I think out of like 9 prints I only had like one or three fail to germinate. I had never even gotten anything viable out of the prints that didn't germinate. Most of them popped off between 2-3 weeks time.
When germinating spores on agar, I have heard it advised to use 1.5% agar powder for softer, wetter agar. I did some on 2% standard plates and most on 1.5% plates (all pda.) The 2% plates did germinate a bit slower, but it was a different print and there was less water on the surface, so the impact is uncertain.
I would only move to making syringes or spore solution as a last resort.
https://www.shroomery.org/forums/showflat.php/Number/21176491 My favorite resource on agar composition. Check out pastywhytes easy agar tek for a simple way to make plates, and stropharis and bod have good stuff on how to work with it.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
Edited by Machiavelliavore (04/10/20 04:05 AM)
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bodhisatta 
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26590191 - 04/10/20 04:43 AM (3 years, 9 months ago) |
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Quote:
St.Pedro said: Hi,
I have a small collection of spore prints which are quite old.. They had been kept in individual plastic bags, some again wrapped into tin foil, and all together in a heavy, dark plastic bag, in a tropical, humid place for some 4 to 5 years. How to I best go about to use them? - I have no prior experience in growing, I'll need very detailed guide, links, tips, thank you!


Best way to use them is to use them at all bro...
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St.Pedro
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Re: old spore prints, best way to utilize them ? [Re: gizmo1]
#26590323 - 04/10/20 06:57 AM (3 years, 9 months ago) |
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Quote:
gizmo1 said: Can you get agar locally? Asian markets usually carry it. Health food stores also but it will be more expensive. You can easily find nutes for the agar at a local store, dog food is one option.
sorry late reply, have been VERY busy today..and had no time to look into buying what I shall need.
You are so helpful Gizmo, please bear with me: Yes I also believe that I should get Agar in local supermarkets. Not sure if agar only, how about if it was some powder to prepare a sweet??
"nutes", nutritions, right? dog food?? how about..some yeast powder to make bread? I know that this is available
Quote:
gizmo1 said:You are also going to need a good sized plastic tote to use as a SAB if you dont already have one.
Box Ihave or can easily buy. What as SAB??
Quote:
gizmo1 said: You will neef some sort of container to use as a no pour "petri" this can be small mason jars like half or quarter pint jars, glad mini roumds or snall tupperware. If you get small jars make sure they arent quilted and are clear. If you go with plastic make sure it is pp5(check the recycle code on bottom) to make sure it is autoclavable. Do you have a pressure cooker yet? You will need one to sterilize your agar.
oops, pressure cooker is a must? Sure don't have any here.. Trying to just use pure alcohol to sterilize won't suffice?
Thank you so much Gizmo, I feel I am starting to get there
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St.Pedro
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26590326 - 04/10/20 07:00 AM (3 years, 9 months ago) |
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for those who say it's a waste of time, why I don't buy new ones asf..where I am at I can't, not available, highly illegal
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Machiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26590365 - 04/10/20 07:19 AM (3 years, 9 months ago) |
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You can try BRF cakes (aka PF Tek) without a pressure cooker. However with the age of the prints you don't have the best chances, since any contaminant has prolly 3 weeks to establish itself. Best bet is to buy a pressure cooker and learn to do agar work. Technically it's possible to do agar without a PC, but PF tek doesn't lend itself to inoculation by agar wedge, which means you need to do ground agar in water or liquid culture. Suffice it to say dropping agar wedges into pressure cooked grains will be your easiest path to success.
--------------------
I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
#26590422 - 04/10/20 07:53 AM (3 years, 9 months ago) |
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Quote:
St.Pedro said:
Quote:
gizmo1 said: Can you get agar locally? Asian markets usually carry it. Health food stores also but it will be more expensive. You can easily find nutes for the agar at a local store, dog food is one option.
sorry late reply, have been VERY busy today..and had no time to look into buying what I shall need.
You are so helpful Gizmo, please bear with me: Yes I also believe that I should get Agar in local supermarkets. Not sure if agar only, how about if it was some powder to prepare a sweet??
"nutes", nutritions, right? dog food?? how about..some yeast powder to make bread? I know that this is available
Quote:
gizmo1 said:You are also going to need a good sized plastic tote to use as a SAB if you dont already have one.
Box Ihave or can easily buy. What as SAB??
Quote:
gizmo1 said: You will neef some sort of container to use as a no pour "petri" this can be small mason jars like half or quarter pint jars, glad mini roumds or snall tupperware. If you get small jars make sure they arent quilted and are clear. If you go with plastic make sure it is pp5(check the recycle code on bottom) to make sure it is autoclavable. Do you have a pressure cooker yet? You will need one to sterilize your agar.
oops, pressure cooker is a must? Sure don't have any here.. Trying to just use pure alcohol to sterilize won't suffice?
Thank you so much Gizmo, I feel I am starting to get there 
The powder to prepare a sweet will work as long as it's agar powder and not gelatin. Check the ingredients you want agar powder nothing else.
SAB is a Still air box basically a plastic tub flipped upside down with armholes cut into it. There will be plenty of examples just search it.
And last but not least pressure cooker I would say is 100% necessary. There are other ways around it like a pot with tight fitting lid but who wants to steam sterilize for like 10 hours straight? Not me. Alcohol will not sterilize anything it's a sanitizer. We need sterile not sanitary.
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LogicaL Chaos
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26590536 - 04/10/20 08:53 AM (3 years, 9 months ago) |
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Perhaps use a spore print for an experimental "spore cleaning" technique?
https://www.shroomery.org/forums/showflat.php/Number/26548586#26548586
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St.Pedro
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro] 1
#26590568 - 04/10/20 09:06 AM (3 years, 9 months ago) |
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thank you so much Gizmo!!! That Agar powder, how about if it got sugar and strawberry taste? Or better banana?  I do have an oven!! That should do too, for sterilize, right? That SAB would hardly fit into a pressure cooker, I'd think. In the oven it would, but wouldn't is melt away, even if PP5? Or is it enough, for the SAB, to clean with alcohol, after all? Wouldn't want to loose my sanity anyway.. 
thank you very much for your kind answers Machiavelli and Logical Chaos! Shall look into your suggestions and may come back later with related questions, if such appear
Edited by St.Pedro (04/10/20 09:11 AM)
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Machiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26590629 - 04/10/20 09:42 AM (3 years, 9 months ago) |
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I have sterilized agar in a microwave and it can be done in a boiling water bath as well. It's just kind of a pain in the ass, which is why there aren't really teks for it, it takes a lot of testing. You only need a small pressure cooker for agar, you can probably find one on ebay for like $25 or something, like a 6qt.
PP5 (polypropylene) won't melt at 15psi (252? deg F)
Still air box doesn't need to be sterilized, just wipe it down with 1:10 bleach or 70% Isopropyl every once in a while, and spray soapy water for use. You're just trapping contaminants in liquid and letting anything in the air settle. https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888
Bod has a lot of good info on agar, check his shit out.
--------------------
I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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poisoned
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
#26590894 - 04/10/20 11:45 AM (3 years, 9 months ago) |
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You don't need to sterilize the SAB. Just wash it with soap and use it.
I'd highly recommend you to get pure agar if available. Otherwise, you could probably get that flavored stuff to work.
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St.Pedro
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Re: old spore prints, best way to utilize them ? [Re: poisoned]
#26592635 - 04/11/20 03:14 AM (3 years, 9 months ago) |
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had time for some shopping today, looking quite good, right?

These glass containers with aluminum lids should serve well, and here, in local shop, cost me close to nothing

now time I study up more on how to prepare the agar agar. didn't really get yet how to add nutrients.
Bod, this is Bodhisatta, right? Just found an interesting thread of his..
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poisoned
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26592687 - 04/11/20 04:08 AM (3 years, 9 months ago) |
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Bod = bodhisatta, yes.
There's many ways to prep agar. I like to cook it before PC. Some people just mix the ingredients and put into the PC. I personally also find most recipes a bit strong in both agar and nutrients. 7/7/500 agar/MEA/water works better for me.
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Machiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: poisoned]
#26593234 - 04/11/20 11:01 AM (3 years, 9 months ago) |
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Agar looks like regular agar powder so good there. Those mug-type mason jars may not hold up to the heat, as they are prolly cheap glass not pyrex. Be gentle with them in terms of temperature changes, like put them in a warm water bath before steam sterilizing/pc'ing or they may crack.
--------------------
I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26593358 - 04/11/20 11:58 AM (3 years, 9 months ago) |
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Quote:
poisoned said: Bod = bodhisatta, yes.
There's many ways to prep agar. I like to cook it before PC. Some people just mix the ingredients and put into the PC. I personally also find most recipes a bit strong in both agar and nutrients. 7/7/500 agar/MEA/water works better for me.
If doing no pours you can't just mix and pressure cook you have to heat it and dissolve before pouring into the containers. I agree with the agar recipes being a little on the high side I like to use 8 grams agar and 8 grams malt extract to 500ml I don't notice much difference if any in growth when using less over using 10/10/500 but using less I'm preserving resources if nothing else. I don't really like use less than 8 grams agar to 500ml water because I don't like agar that's on the softer side but I've bee. Thinking about trying sagari. 
Quote:
St.Pedro said: thank you so much Gizmo!!! That Agar powder, how about if it got sugar and strawberry taste? Or better banana?  I do have an oven!! That should do too, for sterilize, right? That SAB would hardly fit into a pressure cooker, I'd think. In the oven it would, but wouldn't is melt away, even if PP5? Or is it enough, for the SAB, to clean with alcohol, after all? Wouldn't want to loose my sanity anyway.. 
thank you very much for your kind answers Machiavelli and Logical Chaos! Shall look into your suggestions and may come back later with related questions, if such appear
Lmao no. You won't be sterilizing the SAB I don't even wipe mine with alcohol beforehand. Just loaf it with what I'm working on and wait for air to settle before going to work.
Also I don't see anything to use as nutrients for your agar in that pic do you know what kind of agar you will be making yet? Dry dog food is supposed to work pretty good potatoes water or potato flakes also both should both be available at local stores. I don't have much experience with either so I can't really give recipes. I've always just used MEA and grain soak water for the most part, but it should be pretty easy to find a good recipe for either of you use the search function.
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poisoned
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
#26593448 - 04/11/20 12:55 PM (3 years, 9 months ago) |
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Quote:
Machiavelliavore said: Those mug-type mason jars may not hold up to the heat, as they are prolly cheap glass not pyrex.
While proper mason jars are of better quality (less bubbles and impurities), they are not made out of borosilicate glass.
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bodhisatta 
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
#26593546 - 04/11/20 01:35 PM (3 years, 9 months ago) |
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Quote:
Machiavelliavore said: Agar looks like regular agar powder so good there. Those mug-type mason jars may not hold up to the heat, as they are prolly cheap glass not pyrex. Be gentle with them in terms of temperature changes, like put them in a warm water bath before steam sterilizing/pc'ing or they may crack.
Normal glass won't crack unless its subject to a greater than 99° temperature difference suddenly. Mason jars are cheap glass. The cheapest junk glass there is besides the stuff they make windows for india out of
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Machiavelliavore
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Re: old spore prints, best way to utilize them ? [Re: bodhisatta]
#26593567 - 04/11/20 01:44 PM (3 years, 9 months ago) |
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Wellp, guess mason jars are shit. It has been my experience that they crack less than re-used jars from store-bought goods, and I would be a bit careful with those.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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St.Pedro
Stranger

Registered: 03/06/16
Posts: 18
Last seen: 3 years, 5 months
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Re: old spore prints, best way to utilize them ? [Re: Machiavelliavore]
#26624149 - 04/24/20 08:32 AM (3 years, 9 months ago) |
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Sorry good,helpful people I had not come back earlier. Something unexpected had happened that had kept me busy. Two days ago finally I did inoculate the agar dishes. Very primitively, for lack of materials as e.g. a pressure cooker, in case there still will be success I shall report back, thank you again, cheers!
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Wolfenstein
Learning & Yearning


Registered: 03/25/20
Posts: 170
Loc: Here
Last seen: 2 years, 10 months
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Re: old spore prints, best way to utilize them ? [Re: St.Pedro]
#26624809 - 04/24/20 01:45 PM (3 years, 9 months ago) |
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Report back regardless of success. No such thing as a failed experiment
-------------------- Anything and everything I say, write, and/or imply is purely false and is only done so with the intent to enhance my acting career. Molon Labe As above, so below
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