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OldManRiver
Fisherman at large


Registered: 11/12/17
Posts: 416
Loc: Pacific NW USA
Last seen: 21 hours, 11 minutes
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Transfer question, is early advisable?
#26587738 - 04/09/20 12:18 AM (3 years, 9 months ago) |
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Agar newb questions. Haven't done modern cubensis grows, have multiple successes in other mushroom cultivation.
My phone is not letting me upload photos, I think due to lockdown network congestion. I have one plate of cubensis spore syringe spores with 20+ spots of success that are 1/4 to 3/8 in in diameter. I have another plate of APE liquid culture that is spreading out. Both plates are thus far apparently clean, about to get cluttered with sites running into each other. All the sites are small, apparently clean.
My goal is to clean these for some monotub runs. I have plates ready, but don't know when the optimal times are to sample off the initial samples.
Is it better to take samples now, or in a couple days when they are bigger. All seem to be robust. Is there a downside to collecting now, when the colonies are small?
I'm twitchy, please tell me its ok to work now. :-)
[edit, have pics]
Brazilian spores on agar

APE liquid culture on agar
Edited by OldManRiver (04/09/20 12:48 AM)
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Transfer question, is early advisable? [Re: OldManRiver]
#26587793 - 04/09/20 12:57 AM (3 years, 9 months ago) |
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I'd wait a bit too let stronger growth let itself be known since it looks clean. If you see anything that looks like a mold contam starting, I always prefer to transfer before it sporulates, or it may take a few transfers to get rid of it. If you're in a hurry, you can transfer now though, then take transfers from the strongest growth for your future grows later.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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OldManRiver
Fisherman at large


Registered: 11/12/17
Posts: 416
Loc: Pacific NW USA
Last seen: 21 hours, 11 minutes
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Quote:
Machiavelliavore said: I'd wait a bit too let stronger growth let itself be known since it looks clean. If you see anything that looks like a mold contam starting, I always prefer to transfer before it sporulates, or it may take a few transfers to get rid of it. If you're in a hurry, you can transfer now though, then take transfers from the strongest growth for your future grows later.
Not so much in a hurry, as bored from being locked in and wanting to move the project forward. I want to get a a clean, large plate ASAP for each strain. But I will wait for the right time.
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