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OfflineClc420
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Identification on agar (ms inoculation)
    #26586547 - 04/08/20 02:28 PM (3 years, 9 months ago)

So I put some ms solution on a agar plate and they've germinated but I now notice possible bacteria. Is it bacillus? I'll transfer a but if mycelium onto a new one tomorrow but I'm wanting to know what the yellow/brown bit is.



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Offlinebootedboy
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Re: Identification on agar (ms inoculation) [Re: Clc420]
    #26586587 - 04/08/20 02:46 PM (3 years, 9 months ago)

wait for the plate to colonize much more so you can find some clean spots and always look from underneath using a light which you’re doing. I like to shine my phone light under and from above to find the contams and pick the good spots. It always takes a couple transfers to get rid of contams. Good luck!!!


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OfflineClc420
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Re: Identification on agar (ms inoculation) [Re: bootedboy]
    #26586595 - 04/08/20 02:50 PM (3 years, 9 months ago)

Quote:

bootedboy said:
wait for the plate to colonize much more so you can find some clean spots and always look from underneath using a light which you’re doing. I like to shine my phone light under and from above to find the contams and pick the good spots. It always takes a couple transfers to get rid of contams. Good luck!!!




I thought I should transfer sooner so the bacteria hasn't got chance to grow into the myc ? Also say the largest but if myc, would that have multiple sub strains within that? Thank you for your input


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Offlinepoisoned
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Re: Identification on agar (ms inoculation) [Re: Clc420]
    #26586758 - 04/08/20 04:22 PM (3 years, 9 months ago)

Looks a bit like bacteria, but could also be just a stain from the syringe. Observe it for a few days if it grows.


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Offlinerumfor69
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Re: Identification on agar (ms inoculation) [Re: poisoned]
    #26586909 - 04/08/20 05:29 PM (3 years, 9 months ago)

Transfering early to evade contams is a good idea but it's too soon yet


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