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OfflineRidagstran
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Registered: 03/03/19
Posts: 12
Last seen: 3 years, 9 months
Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout
    #26581562 - 04/06/20 12:00 PM (3 years, 10 months ago)

Hey y'all, this is day 13 after spawning. The substrate was drying out due to its location, so it's now been moved and given a good misting. I'm thinking that past misting might have dislodged some of the substrate that was left on the walls of the tub, and that's what's causing the growth you see in the pictures. The air inside the tub still smells humid and fresh. Please let me know what that might be, and if the tub will turn out fine or is done for. Cheers.



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OfflineMystic E

Registered: 09/13/19
Posts: 75
Last seen: 2 months, 23 days
Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: Ridagstran]
    #26583894 - 04/07/20 12:14 PM (3 years, 10 months ago)

Looks fine imo. The yellow stuff is probably just metabolites


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Offlinerumfor69
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Registered: 08/05/11
Posts: 6,842
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Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: Mystic E]
    #26583989 - 04/07/20 01:05 PM (3 years, 10 months ago)

Drying out can cause the mycelium to yellow some. But those spots
almost look like the hulls of your grains or something.

What kind of grain did you use for spawn?


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OfflineSockadin
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Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: rumfor69]
    #26584119 - 04/07/20 01:57 PM (3 years, 10 months ago)

Yeah looks fine. Once your mushrooms grow you won't be able to see it anyway. You should be ok with this one


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OfflineRidagstran
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Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: rumfor69]
    #26584355 - 04/07/20 03:59 PM (3 years, 10 months ago)

I'm being more proactive with misting twice per day, only in the spots that have no visible moisture on the surface (viewed at an angle against a bright light). Whole oats were used per Bod's Oat Tek.

This morning, I noticed that a decent bit of the green has subsided, with yellow metabolites being the main color seen. Having just checked 8 hours later, the green is more prominent again, with bruising easily visible. Could it be the daylight CFL that's aggravating growth, or could it be misting? Apparantly myc is better as fighting contams when properly misted, yet bacteria grows in the water.



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OfflineA.k.aM
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Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: Ridagstran]
    #26584385 - 04/07/20 04:18 PM (3 years, 10 months ago)

Is it just me or is the only yellow from the grain underneath


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Invisiblesh4d0ws
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Registered: 02/26/08
Posts: 12,086
Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: A.k.a]
    #26584391 - 04/07/20 04:20 PM (3 years, 10 months ago)

It kinda looks like a green mold but I am not positive


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Offlinerumfor69
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Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: sh4d0ws]
    #26584538 - 04/07/20 05:38 PM (3 years, 10 months ago)

The big yellow spots are the grains. The blue spots are bruising. But all
them strange off colored greenish/blueish peppered spots everywhere I don't
know what they are.


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OfflineRidagstran
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Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: rumfor69]
    #26584566 - 04/07/20 05:53 PM (3 years, 10 months ago)

My best guess is bacteria. Indeed, the caps of some side pins, specifically where they have been in prolonged contact with the side of the tub, have taken on a slimy-ish yellow appearance. If I'm correct, that's similar to cap rot caused by bacteria.

My main concern at this point is whether or not it will get worse before it gets better, and what affect it will have on yield and future flushes.

Edit: And what I can do to help the mycelium fight it off


Edited by Ridagstran (04/07/20 06:01 PM)


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Offlinerumfor69
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Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: Ridagstran]
    #26584595 - 04/07/20 06:14 PM (3 years, 10 months ago)

There's not anything you can do. Once a tub is mixed up you're locked
in for the ride! Keep us updated with it's progress. Remember the fruits
are just vegetables...don't eat rotten vegetables and you'll be fine.

In the future...it's all about having perfect jars. My recommendations
are clean agar cultures, synthetic filter disk lid ring setups, and
using a flowhood instead of a SAB. I know lots of other things can work
just fine. IME these things were the key to success leaving only technique
as a variable.


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OfflineRidagstran
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Registered: 03/03/19
Posts: 12
Last seen: 3 years, 9 months
Re: Is this metabolites or contam? Small Greenish Yellowish Blotches Throughout [Re: rumfor69]
    #26588922 - 04/09/20 01:54 PM (3 years, 10 months ago)

Update! Looks like most of the green has subsided and only some yellow remains. I've been keeping that room around 69 degrees to lessen bacterial growth. I will be raising the temp in the coming days as the mycelium gains a stronger hold over the bacteria.


The tub is now looking like this:


A few caps had separated from their veils, so I harvested them. The caps had somewhat of a slimy, sticky feel, but smelled normal. A couple had this yellowish liquid at the base, probably metabolites. They are in the dehydrator now.


Hopefully, if this flush is going to be small due to the contamination (and non-ideal climate conditions), the next one will be bigger.


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