Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Boomr Bag   Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
OfflineStonedApeAquarian
Stranger
 User Gallery
Registered: 10/20/18
Posts: 15
Last seen: 2 years, 10 months
Why do I keep getting contams?
    #26583429 - 04/07/20 08:14 AM (3 years, 9 months ago)

I’ve lost a couple tubs: 1 monotub and 2 dubtubs. From what I’ve read, it’s harder for contams to take hold once the mycelium is fully colonized. I’ve been doing grain to grain transfers using WBS. All my jars have fully colonized with no signs of any contams, just strong healthy mycelium. I case using coco coir/vermiculite. To make my bulk substrate, i soak the mix in boiling water. I’m thinking maybe I should try to sterilize my substrate somehow ?? I never make it to fruiting. Once my tubs are almost fully colonized, I see contams growing and I have to toss them. Any ideas on what might be going wrong or what I can do to try to prevent it from happening next time?


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 21 minutes, 20 seconds
Re: Why do I keep getting contams? [Re: StonedApeAquarian] * 1
    #26583453 - 04/07/20 08:30 AM (3 years, 9 months ago)

What kinda contamination are you seeing in your almost fully colonized tubs?

What is your spawn to mix ratio?

Are you following a bucket tek for your coir/verm prep?

Is your coir/verm mix at the correct field capacity?
(Hard squeeze and a little water trickles out then drips)

Typically contamination before the first flush is a problem with the jars

Are you using a clean culture from agar to make master jars or spore solution?

What is the lid setup on your jars?

What tub design are you using?
(modified, unmodified)


--------------------

Links For Beginners And Beyond


Extras: Filter Print Post Top
InvisibleThe_Brown_Wizard
what?
Male


Registered: 12/27/19
Posts: 188
Loc: Southeastern Carpathians Flag
Re: Why do I keep getting contams? [Re: StonedApeAquarian]
    #26583455 - 04/07/20 08:32 AM (3 years, 9 months ago)

You need to pasteurize coir so that you only kill bad organisms and let the beneficial ones live, sterilizing it is actually bad because you are killing everything, thus making room for all the nasties to come in

If the contam doesn't seem to be too bad you can try to keep them and put to fruiting as some will still produce fruits, even if contaminated


--------------------
:scaryshroom: There's a weird fuck lurking in these woods :scaryshroom:


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 21 minutes, 20 seconds
Re: Why do I keep getting contams? [Re: The_Brown_Wizard]
    #26583459 - 04/07/20 08:37 AM (3 years, 9 months ago)

I sterilize my coir/verm in an electric boiler steam box I built. 166°F
for 8hrs. It's worked perfectly for years. That leave the "good things"
by pasteurizing doesn't apply to coir/verm. It's heated up all to fuck
when it's made and bricked.


--------------------

Links For Beginners And Beyond


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
Trusted Cultivator
Re: Why do I keep getting contams? [Re: rumfor69]
    #26583514 - 04/07/20 09:15 AM (3 years, 9 months ago)

You can Sterilize, pasteurize, faux Pasteurize. Doesn't matter. I think the brand matters more. I prefer to Sterilize.


Extras: Filter Print Post Top
InvisibleThe_Brown_Wizard
what?
Male


Registered: 12/27/19
Posts: 188
Loc: Southeastern Carpathians Flag
Re: Why do I keep getting contams? [Re: rumfor69]
    #26583539 - 04/07/20 09:26 AM (3 years, 9 months ago)

Quote:

rumfor69 said:
I sterilize my coir/verm in an electric boiler steam box I built. 166°F
for 8hrs.




Isn't keeping something at that temperature considered pasteurizing?
"Sterilization is the probability that all bacteria, spores, fungi and viruses are killed or inactivated"
That temp will not kill everything

Most (if not all) trusted cultivators on the shroomery seem to all agree that sterilizing bulk substrates is borderline bad


--------------------
:scaryshroom: There's a weird fuck lurking in these woods :scaryshroom:


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 21 minutes, 20 seconds
Re: Why do I keep getting contams? [Re: The_Brown_Wizard]
    #26583584 - 04/07/20 09:46 AM (3 years, 9 months ago)

Once you start getting over 145°F area you're reaching sterilizing
temperatures. Then it's just a matter of time extension to reach sterility.
The same way a 2psi steel drum sterilizer can sterilize
blocks of sawdust and woodchips even though it's not at pressure cooker
temps. You just have to run it for longer.


--------------------

Links For Beginners And Beyond


Extras: Filter Print Post Top
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 1,670
Re: Why do I keep getting contams? [Re: StonedApeAquarian]
    #26583601 - 04/07/20 09:53 AM (3 years, 9 months ago)

Quote:

StonedApeAquarian said:
I’ve lost a couple tubs: 1 monotub and 2 dubtubs. From what I’ve read, it’s harder for contams to take hold once the mycelium is fully colonized. I’ve been doing grain to grain transfers using WBS. All my jars have fully colonized with no signs of any contams, just strong healthy mycelium. I case using coco coir/vermiculite. To make my bulk substrate, i soak the mix in boiling water. I’m thinking maybe I should try to sterilize my substrate somehow ?? I never make it to fruiting. Once my tubs are almost fully colonized, I see contams growing and I have to toss them. Any ideas on what might be going wrong or what I can do to try to prevent it from happening next time?




It's your spawn. Likely due to contaminated inoculate.
Got plate pics?


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Why do I keep getting contams? [Re: The_Brown_Wizard]
    #26583621 - 04/07/20 10:04 AM (3 years, 9 months ago)

Quote:

The_Brown_Wizard said:
Quote:

rumfor69 said:
I sterilize my coir/verm in an electric boiler steam box I built. 166°F
for 8hrs.




Isn't keeping something at that temperature considered pasteurizing?
"Sterilization is the probability that all bacteria, spores, fungi and viruses are killed or inactivated"
That temp will not kill everything

Most (if not all) trusted cultivators on the shroomery seem to all agree that sterilizing bulk substrates is borderline bad




That isn't true at all. If anything, all TCs agree that coir can be sterilized,
partially sterilized, or even hydrated with cold water skipping any heat treatment whatsoever.

Ask any TC and they will say that if you use coir or coir+verm and your tub contammed
then it was almost certainly your spawn that is to blame.

Don't necessarily believe that 100% myself but it is the commonly held belief
on this forum


--------------------


Extras: Filter Print Post Top
InvisibleThe_Brown_Wizard
what?
Male


Registered: 12/27/19
Posts: 188
Loc: Southeastern Carpathians Flag
Re: Why do I keep getting contams? [Re: natedawgnow]
    #26583673 - 04/07/20 10:34 AM (3 years, 9 months ago)

Quote:

rumfor69 said:
Once you start getting over 145°F area you're reaching sterilizing
temperatures. Then it's just a matter of time extension to reach sterility.




I didn't know sterilizing like that was a thing ; it does make quite a lot of sense now

Quote:

natedawgnow said:

That isn't true at all. If anything, all TCs agree that coir can be sterilized,
partially sterilized, or even hydrated with cold water skipping any heat treatment whatsoever.





After doing some digging on the forums I found TCs saying sterilizing is ok. (usually I saw they recommended pasteurizing :shrug: )

well.. guess i was wrong as fuck


--------------------
:scaryshroom: There's a weird fuck lurking in these woods :scaryshroom:


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 57 minutes
Trusted Cultivator
Re: Why do I keep getting contams? [Re: The_Brown_Wizard]
    #26583716 - 04/07/20 10:53 AM (3 years, 9 months ago)

With manure or other highly nutritious subs then sterilization is bad.

Coir doesn’t matter, it shouldn’t have beneficial microbes or any microbes in it.


I was paranoid with coir at first but I’ve never had just coir by itself go bad yet.

I always just pour boiling water in a bucket, but I’ve used cold water too no problem.


--------------------
LAGM2020


Extras: Filter Print Post Top
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 1,670
Re: Why do I keep getting contams? [Re: A.k.a]
    #26583764 - 04/07/20 11:12 AM (3 years, 9 months ago)

I've always wondered why people bother with the bucket.
I put my coir in the pot with the water that just came to a boil, wait till cool, then add spawn to same pot and mix. One pot to rule them all.


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Why do I keep getting contams? [Re: LadysKnight]
    #26583773 - 04/07/20 11:16 AM (3 years, 9 months ago)

Hard to use 1 pot when you're prepping 10 tubs at a time.

I just load 5 bricks in a 66qt at a time and boil 5 tubs worth of water and dump it in.


--------------------


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 21 minutes, 20 seconds
Re: Why do I keep getting contams? [Re: natedawgnow]
    #26583796 - 04/07/20 11:29 AM (3 years, 9 months ago)

I'm thinking about making one of these some day



--------------------

Links For Beginners And Beyond


Extras: Filter Print Post Top
OfflineSockadin
Male User Gallery


Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
Trusted Cultivator
Re: Why do I keep getting contams? [Re: A.k.a]
    #26583801 - 04/07/20 11:31 AM (3 years, 9 months ago)

Ha e You checked out Cronicr s in Pasteurize spawn run? It is interesting.sh shit and bugs.


Extras: Filter Print Post Top
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 1,670
Re: Why do I keep getting contams? [Re: natedawgnow]
    #26583873 - 04/07/20 12:04 PM (3 years, 9 months ago)

Quote:

natedawgnow said:
Hard to use 1 pot when you're prepping 10 tubs at a time.

I just load 5 bricks in a 66qt at a time and boil 5 tubs worth of water and dump it in.




Yeah true. It's a 6gal pot, I've done as many as 7 tubs at a time, but typically shoot for 5.


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Why do I keep getting contams? [Re: LadysKnight]
    #26583903 - 04/07/20 12:18 PM (3 years, 9 months ago)

7 full 66qt tubs at a time using a 6gal pot? How is that even possible the coir would
for sure overflow that pot if you're putting bricks in it while water is boiling.

I can only fit one brick fully expanded in a 5gal bucket


--------------------


Extras: Filter Print Post Top
InvisibleLadysKnight
Hello Ladies
 User Gallery

Registered: 10/09/15
Posts: 1,670
Re: Why do I keep getting contams? [Re: natedawgnow]
    #26584035 - 04/07/20 01:28 PM (3 years, 9 months ago)

Sorry, no, I meant shoeboxes


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Boomr Bag   Mushroom-Hut Grow Bags   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* masking tape vs electrical tape *DELETED*
( 1 2 all )
Adden 5,151 35 05/29/03 06:25 PM
by LiL_KuSsH
* Anybody use an electric PC on a gas flame? Fred Garvin 1,344 4 10/29/02 04:31 PM
by Fred Garvin
* Cleaning tubing/contams MushroomMatt 803 1 10/14/01 11:54 AM
by FungusFarmer
* Using Electric Blanket to Control Temp? ChopperDave 2,170 18 08/11/03 10:16 PM
by Ravinoff
* Reduce contams with jars- extra step.
( 1 2 all )
aenima 5,659 22 05/25/02 02:49 PM
by groovysunrise
* Electric Roaster Oven For Sterilization EnCHaNTeDPiXiE 2,265 15 08/24/03 03:57 PM
by Senovio
* Re: Early Contam Detection w/Blacklight hitterg 1,046 7 06/04/00 03:48 AM
by Krupa
* electric pc's *DELETED* djred 821 7 11/19/04 09:53 PM
by djred

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
299 topic views. 29 members, 216 guests and 45 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.008 seconds on 14 queries.