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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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First time LC question
#26583027 - 04/06/20 11:59 PM (3 years, 10 months ago) |
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Here some LC I figure is ready for a test trial on agar let me know your thoughts first run on LC and got a couple jars going this one is farther along(I believe) going straight into 4.5qt millet spawn bag if get the thumbs up. Quick question here I acquire 3in blunt needles and 16 gauge pointed needles I don’t think either will reach this 400 ml of LC although seen many people recommend blunts is there a way to get all this out or I’m I going have transfer? Also got 60cc syringe is this good use right out of package and how do you recommend sterilizing after first use?

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Edited by PiptheGreAtest (04/07/20 12:17 AM)
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 18 days
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Those 3" LC syringes arent usually shipped sterile fyi. Next bottle of agar or lc wrap the 3" in foil and lay it on the top of the jars then its ready when you need it.
Just aspirate that jar, flame your 16ga, insert, tilt, draw. You are using syringe filters and ships so you can turn it upside down all you want.
Yes your syringe is good out of the package.
After use :
To clean just Boil water . Draw into syringe and plunge it 2xs on 3rd time let water sit in etc
Before reuse:
1. You fill syringe with water drop into big pot and boil for few mins then squirt- flame- use.
2. Press all air out. Put on a syringe cap or needle+cap place into mason jar PC with next Lc or agar run. If youre paranoid like me when cycle is done put a lid on it to keep contams from getting on the inner barrel behind the plunger or use a poly lid during etc. You could just put it in a ziplock but i dont trust those leaky fucks. Im kidding of course. Either way works.
Edited by shevanel (04/07/20 01:31 AM)
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: First time LC question [Re: shevanel]
#26583101 - 04/07/20 01:06 AM (3 years, 10 months ago) |
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Quote:
shevanel said: Those 3" LC syringes arent usually shipped sterile fyi. Next bottle of agar or lc wrap the 3" in foil and lay it on the top of the jars.
Just aspirate that jar, flame your 16ga, insert, tilt, draw.
Yes your syringe is good out of the package.
After use : boil some water. Suck it up into the syringe and plunge it out 2x then on 3rd time leave the water in. Next time you use it just plunge the water.
Noted will put in jar or something to pc before use. Is it recommend to draw water and runsyringe through pc cycle?( I be hesitant with boiling water because I’m doing a high volume of bags but it’s well founded than I’d give it a try)Any recommendations on how to get to bottom jar( duh turn upside Down lol!! Correct?)
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Edited by PiptheGreAtest (04/07/20 01:17 AM)
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 18 days
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Syringes steralized in boiling water is common practice. Running them in the pressure cooker is more of a convenience than a necessity.
Edited by shevanel (04/07/20 01:22 AM)
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: First time LC question [Re: shevanel]
#26583424 - 04/07/20 08:09 AM (3 years, 10 months ago) |
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Quote:
shevanel said: Those 3" LC syringes arent usually shipped sterile fyi. Next bottle of agar or lc wrap the 3" in foil and lay it on the top of the jars then its ready when you need it.
Just aspirate that jar, flame your 16ga, insert, tilt, draw. You are using syringe filters and ships so you can turn it upside down all you want.
Yes your syringe is good out of the package.
After use :
To clean just Boil water . Draw into syringe and plunge it 2xs on 3rd time let water sit in etc
Before reuse:
1. You fill syringe with water drop into big pot and boil for few mins then squirt- flame- use.
2. Press all air out. Put on a syringe cap or needle+cap place into mason jar PC with next Lc or agar run. If youre paranoid like me when cycle is done put a lid on it to keep contams from getting on the inner barrel behind the plunger or use a poly lid during etc. You could just put it in a ziplock but i dont trust those leaky fucks. Im kidding of course. Either way works.
On the last point you mean inner barrel of needle?
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 3 months, 8 days
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Behind the plunger. The area that is exposed to open air.
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 18 days
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Nah, when the plunger is pushed fully in youll notice the part of the syringe behind the plunger it exposed to open air.
Usually not a big deal but i dont like leaving them in open air like that.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 48 minutes
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Re: First time LC question [Re: shevanel]
#26583545 - 04/07/20 09:27 AM (3 years, 10 months ago) |
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I used a needle for lc the first couple times but just pouring it is so much easier.
Yours looks good. All mine that went bad got cloudy so it was easy to tell.
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LAGM2020     
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PiptheGreAtest
Aspiring Heavyweight


Registered: 07/18/16
Posts: 464
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Re: First time LC question [Re: A.k.a]
#26583927 - 04/07/20 12:31 PM (3 years, 10 months ago) |
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So cloudy is bad sign got one that is hard see in good to know.
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 2 hours, 48 minutes
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Yeah here’s a bad cloudy one next to a healthy one
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LAGM2020     
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