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OfflineDdaShroom
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Registered: 05/16/19
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Growing shrooms from just a spore print HELP
    #26581744 - 04/06/20 01:26 PM (3 years, 9 months ago)

Hello,

So I have a mushroom about to drop a spore print. I want to eventually grow a bunch of the species from this spore print.

How much spore print do I need?
What do I do once I have the print?


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OfflineMystic E

Registered: 09/13/19
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Re: Growing shrooms from just a spore print HELP *DELETED* [Re: DdaShroom]
    #26581761 - 04/06/20 01:36 PM (3 years, 9 months ago)

Post deleted by Mystic E

Reason for deletion: .


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Invisiblestaytrippy420
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Re: Growing shrooms from just a spore print HELP [Re: Mystic E]
    #26581781 - 04/06/20 01:43 PM (3 years, 9 months ago)

Clone a fruit on agar. :headbang3:


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Tek's I use

LAGM2020


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Onlinerumfor69
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Registered: 08/05/11
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Re: Growing shrooms from just a spore print HELP [Re: staytrippy420]
    #26582004 - 04/06/20 03:42 PM (3 years, 9 months ago)

Or at least make spore syringes so you can keep doing stuff but I
suggest cloned fruits and spores on agar also


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OfflineDdaShroom
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Re: Growing shrooms from just a spore print HELP [Re: rumfor69]
    #26582965 - 04/06/20 10:51 PM (3 years, 9 months ago)

Quote:

rumfor69 said:
Or at least make spore syringes so you can keep doing stuff but I
suggest cloned fruits and spores on agar also




Ok so it seems like cloning fruit on agar is the resounding winner?

Could of questions still (thank you all a ton btw).

So, where do I get the agar? Where do I get the petri dishes? Is there anything else I would need to assure sterilization? What do I do after the cloned fruit produces mycelium within the agar dish? Thank you!


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OfflineDdaShroom
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Registered: 05/16/19
Posts: 584
Last seen: 1 year, 5 months
Re: Growing shrooms from just a spore print HELP [Re: Mystic E]
    #26582967 - 04/06/20 10:52 PM (3 years, 9 months ago)

Ok so it seems like cloning fruit on agar is the resounding winner?

Could of questions still (thank you all a ton btw).

So, where do I get the agar? Where do I get the petri dishes? Is there anything else I would need to assure sterilization? What do I do after the cloned fruit produces mycelium within the agar dish? Thank you!


Extras: Filter Print Post Top
Onlinerumfor69
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Registered: 08/05/11
Posts: 6,767
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Last seen: 1 minute, 54 seconds
Re: Growing shrooms from just a spore print HELP [Re: DdaShroom]
    #26583121 - 04/07/20 01:35 AM (3 years, 9 months ago)

So here is a bunch of info on agar I suggest reading through it and
learning different teks just for knowledge sake. It's kind of a lot
of stuff for beginners. Definitely watch RR's videos and others you
can find on working with agar so you know what you're getting into.
https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954

Personally I take a very easy method to do agar.
I buy:
Potato Dextrose Agar(PDA) off Amazon $16

Synthetic filter disks 90mm wide mouth from this sponsor http://www.mycosupply.com/
$1 a piece and can be used over and over and you will need them
for grain jars in the future so I'd buy a dozen or more

Then a 12 pack of wide mouth half pint jars around $12

Poke two 1/8" holes in the metal lid from the rubber seal side so the
burrs stick out the metal side.

Mix 1.17g of PDA with 30ml water in one of the jars. Microwave on high
till it just barely starts to bubble. Take out and stir it some. Then
put on the metal lid with the rubber seal up, then a filter disk, then
the screw ring. Cover with a piece of tinfoil and pressure cook for 15mins @15psi.

After it cools down all the way you can remove the tinfoil and let it
sit for a few days to make sure it's sterile and for condensation to
work out of it.

Then your ready to flame sterilize your scalpel, and take a sample of
flesh from the inside of a freshly split stem, and place in on the agar
While working in your SAB or flowhood.


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