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Offlinemcminc
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Registered: 04/06/20
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Help needed, no mycelium growth in jars
    #26581510 - 04/06/20 11:35 AM (3 years, 9 months ago)

I inoculated 6 brf jars over 2 weeks ago and so far haven’t seen any signs of growth. Am too broke to buy a PC although I understand there usefulness. I steam sterilized for 45 minutes. After waiting for two weeks I see absolutely no signs of contamination but also no signs of myc growth. The only thing I can think of is that my house has relatively cold temperatures for incubation.

Over these two weeks I had a thermometer with the jars and it recorded a min temp of 63 f and a max of 73f

Any advice would be much appreciated! I am not sure what I am doing wrong


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OfflineDomno
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Registered: 01/30/20
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Re: Help needed, no mycelium growth in jars [Re: mcminc]
    #26581602 - 04/06/20 12:17 PM (3 years, 9 months ago)

63 sounds a little low. I have all of my grow stuff in a small room and just keep a space heater in there to keep it in in the low 70s.

It may not be a bad idea to start playing with agar. You can still go syringe to jar but it may be nice to have more of a sure thing growing as well. It sounds intimidating, but just like driving, it's only complicated until it's not, then never again. Also a syringe may inoculate 5 or 10 jars, but it will also inoculate like a hundred agar plates/cups.


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OfflineIceKing
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Registered: 03/09/13
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Re: Help needed, no mycelium growth in jars [Re: Domno]
    #26581738 - 04/06/20 01:24 PM (3 years, 9 months ago)

Give it a little more time, I've had spores take three weeks to show grow. It'll take longer in colder conditions too, like Domno says, try and warm things up a bit.


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Offlinerumfor69
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Re: Help needed, no mycelium growth in jars [Re: IceKing]
    #26581993 - 04/06/20 03:38 PM (3 years, 9 months ago)

Room temps that you're comfortable in are fine for them. You don't have
to incubate unless absolutely necessary like if you live in a fridge or something lol
Just gotta wait and see longer. If they do nothing ever maybe it was a
bad syringe, maybe a contamination you can't see, maybe the tek your following,
could be a few things. Take the tin foil off them incase you didn't.
Some good close pics from the sides might help too


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Offlinemcminc
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Registered: 04/06/20
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Re: Help needed, no mycelium growth in jars [Re: rumfor69]
    #26584185 - 04/07/20 02:22 PM (3 years, 9 months ago)

Thanks for all the input, I’m not a very patient person but hopefully I might learn some patience from this hobby. Spores are from a long time sponsor that is usually reliable and I don’t think they were bad because I made honey LC with the same syringe and it turned out fine.

I have a temperature regulated seedling heating mat coming in the mail so hopefully that will help:grin:


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Offlinepoisoned
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Registered: 04/17/13
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Re: Help needed, no mycelium growth in jars [Re: mcminc]
    #26584189 - 04/07/20 02:25 PM (3 years, 9 months ago)

It'll only help you to fuck up those jars


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Offlinemcminc
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Registered: 04/06/20
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Re: Help needed, no mycelium growth in jars [Re: poisoned]
    #26584524 - 04/07/20 05:29 PM (3 years, 9 months ago)

How so? temperatures are too inconsistent where I live right now and my only other option is using a space heater


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Offlinerumfor69
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Re: Help needed, no mycelium growth in jars [Re: mcminc] * 1
    #26584578 - 04/07/20 06:01 PM (3 years, 9 months ago)

It doesn't care if the temp goes up and down and it likes cooler temps
even. Contamination loves it warmer. You don't wanna incubate jars.
Cooler temps close to 60°F may make them grow a little slower during
then but not even anything that's noticeable. They make their own heat
in the jars. They're warmer inside than the surrounding ambient temp.


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Offlinemcminc
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Registered: 04/06/20
Posts: 6
Last seen: 3 years, 5 months
Re: Help needed, no mycelium growth in jars [Re: rumfor69]
    #26595896 - 04/12/20 02:20 PM (3 years, 9 months ago)

My first jars never took hold but while waiting for them I started a new batch and they’re coming along nicely. (yes I know i’m using the shitty kind of jars)





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