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mcminc
Stranger
Registered: 04/06/20
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Help needed, no mycelium growth in jars
#26581510 - 04/06/20 11:35 AM (3 years, 9 months ago) |
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I inoculated 6 brf jars over 2 weeks ago and so far haven’t seen any signs of growth. Am too broke to buy a PC although I understand there usefulness. I steam sterilized for 45 minutes. After waiting for two weeks I see absolutely no signs of contamination but also no signs of myc growth. The only thing I can think of is that my house has relatively cold temperatures for incubation.
Over these two weeks I had a thermometer with the jars and it recorded a min temp of 63 f and a max of 73f
Any advice would be much appreciated! I am not sure what I am doing wrong
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Domno
Magician



Registered: 01/30/20
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Re: Help needed, no mycelium growth in jars [Re: mcminc]
#26581602 - 04/06/20 12:17 PM (3 years, 9 months ago) |
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63 sounds a little low. I have all of my grow stuff in a small room and just keep a space heater in there to keep it in in the low 70s.
It may not be a bad idea to start playing with agar. You can still go syringe to jar but it may be nice to have more of a sure thing growing as well. It sounds intimidating, but just like driving, it's only complicated until it's not, then never again. Also a syringe may inoculate 5 or 10 jars, but it will also inoculate like a hundred agar plates/cups.
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IceKing
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Re: Help needed, no mycelium growth in jars [Re: Domno]
#26581738 - 04/06/20 01:24 PM (3 years, 9 months ago) |
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Give it a little more time, I've had spores take three weeks to show grow. It'll take longer in colder conditions too, like Domno says, try and warm things up a bit.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
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Re: Help needed, no mycelium growth in jars [Re: IceKing]
#26581993 - 04/06/20 03:38 PM (3 years, 9 months ago) |
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Room temps that you're comfortable in are fine for them. You don't have to incubate unless absolutely necessary like if you live in a fridge or something lol Just gotta wait and see longer. If they do nothing ever maybe it was a bad syringe, maybe a contamination you can't see, maybe the tek your following, could be a few things. Take the tin foil off them incase you didn't. Some good close pics from the sides might help too
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mcminc
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Registered: 04/06/20
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Re: Help needed, no mycelium growth in jars [Re: rumfor69]
#26584185 - 04/07/20 02:22 PM (3 years, 9 months ago) |
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Thanks for all the input, I’m not a very patient person but hopefully I might learn some patience from this hobby. Spores are from a long time sponsor that is usually reliable and I don’t think they were bad because I made honey LC with the same syringe and it turned out fine.
I have a temperature regulated seedling heating mat coming in the mail so hopefully that will help
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poisoned
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Registered: 04/17/13
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Re: Help needed, no mycelium growth in jars [Re: mcminc]
#26584189 - 04/07/20 02:25 PM (3 years, 9 months ago) |
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It'll only help you to fuck up those jars
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mcminc
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Re: Help needed, no mycelium growth in jars [Re: poisoned]
#26584524 - 04/07/20 05:29 PM (3 years, 9 months ago) |
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How so? temperatures are too inconsistent where I live right now and my only other option is using a space heater
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
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Re: Help needed, no mycelium growth in jars [Re: mcminc] 1
#26584578 - 04/07/20 06:01 PM (3 years, 9 months ago) |
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It doesn't care if the temp goes up and down and it likes cooler temps even. Contamination loves it warmer. You don't wanna incubate jars. Cooler temps close to 60°F may make them grow a little slower during then but not even anything that's noticeable. They make their own heat in the jars. They're warmer inside than the surrounding ambient temp.
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mcminc
Stranger
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Last seen: 3 years, 5 months
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Re: Help needed, no mycelium growth in jars [Re: rumfor69]
#26595896 - 04/12/20 02:20 PM (3 years, 9 months ago) |
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My first jars never took hold but while waiting for them I started a new batch and they’re coming along nicely. (yes I know i’m using the shitty kind of jars)



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