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Offlineigorcarajo
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That goddamn trichoderma...
    #26581389 - 04/06/20 10:25 AM (3 years, 9 months ago)

First grow (using PF-BRF tek) I made 12 cakes, inoculated with a spore syringe purchased from a vendor, all 12 cakes perfectly colonized with mycelium, no contamination. After that, when I harvested the fruits, I made a spore print. Later I used the spore print to make a new syringe and did a new batch of cakes. This time 9 out of the 12 cakes got invaded by the goddamn trichoderma mold. I’m thinking that the spore print was contaminated. So my question is: how do you make a spore print and then a syringe without trichoderma contaminating it? Thanks.


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OfflineA.k.aM
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Re: That goddamn trichoderma... [Re: igorcarajo]
    #26581399 - 04/06/20 10:31 AM (3 years, 9 months ago)

Look up print teks.

You gotta be careful to keep everything sterile.

I think the one where you bake the foil then put a grid over it in a SAB is your best bet.


--------------------
LAGM2020


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Offlineigorcarajo
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Re: That goddamn trichoderma... [Re: A.k.a]
    #26581567 - 04/06/20 12:03 PM (3 years, 9 months ago)

But how do you prevent trichoderma spores from getting on the mushroom caps? I mean, the caps are just growing inside a box with holes. It’s likely that there are mold spores attached to the caps, then they get on the foil. Is there any way to kill the trichoderma spores on the foil without killing the mushroom spores?


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OfflineMooshi97
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Re: That goddamn trichoderma... [Re: igorcarajo]
    #26581587 - 04/06/20 12:10 PM (3 years, 9 months ago)

Quote:

igorcarajo said:
But how do you prevent trichoderma spores from getting on the mushroom caps? I mean, the caps are just growing inside a box with holes. It’s likely that there are mold spores attached to the caps, then they get on the foil. Is there any way to kill the trichoderma spores on the foil without killing the mushroom spores?



You have to do a double print . U let the first spores drop in a sab which is usually the ones with contaminates then put the caps on a new piece of foil and leave for a day or more for the second round of spores which weren't exposed to open air. Look up bhods spore print tek


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OfflineMugnath
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Registered: 05/24/12
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Re: That goddamn trichoderma... [Re: igorcarajo]
    #26581609 - 04/06/20 12:20 PM (3 years, 9 months ago)

You aren't going to achieve perfection. Your technique will help minimize competition on your print though.


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