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Offlineahatchingegg
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Registered: 03/10/20
Posts: 71
Last seen: 9 months, 24 days
Agar transfer tool * 1
    #26580591 - 04/05/20 09:41 PM (3 years, 9 months ago)

It came to me in a dream, and when I looked online, I found it. Has anyone used such a thing? https://www.amazon.com/Robbins-Instruments-Disposable-Biopsy-Punches/dp/B00JRK87QS


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Offlinederek420
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Registered: 12/20/19
Posts: 49
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Re: Agar transfer tool [Re: ahatchingegg]
    #26580609 - 04/05/20 09:48 PM (3 years, 9 months ago)

Those are pretty cool. But honestly you can do the same thing with a needle. Just poke the agar with the needle then squirt it onto another Piece of agar. You literally only need one cell of the myc and it’ll transfer and grow. Someone here has a tek on it but I can’t remember who.


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OfflineMachiavelliavore
Vermiculite Hater
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Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
Re: Agar transfer tool [Re: ahatchingegg]
    #26580612 - 04/05/20 09:50 PM (3 years, 9 months ago)

If you can sterilize and re-use them I guess they'd be pretty cool.  If not, paying $2 per transfer is gonna add up fast.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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OfflineMycoBrainz
Mycotic
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Registered: 03/14/15
Posts: 615
Loc: West Coast
Last seen: 4 months, 10 days
Re: Agar transfer tool [Re: Machiavelliavore]
    #26580626 - 04/05/20 10:00 PM (3 years, 9 months ago)

Stainless steel spatula


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:stoned:


PFC x Creeper


Lets Get Stoned


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Invisiblewildernessjunkie
Reshitivest
I'm a teapot

Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
Trusted Cultivator
Re: Agar transfer tool [Re: Machiavelliavore] * 1
    #26580627 - 04/05/20 10:00 PM (3 years, 9 months ago)

Theres a user over on the FB group named Misty. She uses leather punches. But I wouldn't use that thing, especially since its disposable, and since you can cut a wedge faster and re use the scalpel.


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Offlineahatchingegg
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Registered: 03/10/20
Posts: 71
Last seen: 9 months, 24 days
Re: Agar transfer tool [Re: derek420]
    #26580670 - 04/05/20 10:22 PM (3 years, 9 months ago)

I imagine a larger sample would colonize faster. Am I mistaken? Or, is there a tradeoff in colonizing faster?


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OfflineHamHead
Hard Ass Motherfucker
Male


Registered: 03/17/15
Posts: 6,107
Loc: Galactic sector ZZ9 Plura...
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Re: Agar transfer tool [Re: ahatchingegg] * 1
    #26580676 - 04/05/20 10:28 PM (3 years, 9 months ago)

A larger sample would provide more chance for contamination to be taken along with.

A grain of rice is all that's needed, and I've gotten away with far less.

Especially if going for an isolate, I would imagine an extremely small sample would be more desirable than a larger one.

:takingnotes:


--------------------
The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February.

https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF

This online first version has been peer-reviewed, accepted and edited,  but not formatted and finalized with corrections from authors and proofreaders

https://www.icandecide.org/


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InvisibleStrainsfordaze


Registered: 05/10/18
Posts: 669
Re: Agar transfer tool [Re: ahatchingegg] * 1
    #26580707 - 04/05/20 10:48 PM (3 years, 9 months ago)

I have tried biopsy punches and cork borers. Scalpel is the way I prefer.


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OfflineBabylon
Shaman


Registered: 05/15/11
Posts: 442
Last seen: 2 years, 8 months
Re: Agar transfer tool [Re: derek420]
    #26580717 - 04/05/20 10:52 PM (3 years, 9 months ago)

Quote:

derek420 said:
Those are pretty cool. But honestly you can do the same thing with a needle. Just poke the agar with the needle then squirt it onto another Piece of agar. You literally only need one cell of the myc and it’ll transfer and grow. Someone here has a tek on it but I can’t remember who.





Josex is the user with the biopsy tek.


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Offlinederek420
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Re: Agar transfer tool [Re: Babylon]
    #26580893 - 04/06/20 01:22 AM (3 years, 9 months ago)

Ah yes that’s who it was. Thanks


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InvisibleSunny Skies
Cluster Head
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Registered: 05/03/17
Posts: 421
Loc: my house
Re: Agar transfer tool [Re: wildernessjunkie]
    #26580999 - 04/06/20 04:18 AM (3 years, 9 months ago)

Yes she uses a leather whole pouch...she does beautiful work I must say...had to get off the facebook page..couple people there I know and wanted to stay on the DL


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Offlineahatchingegg
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Registered: 03/10/20
Posts: 71
Last seen: 9 months, 24 days
Re: Agar transfer tool [Re: Machiavelliavore]
    #26581317 - 04/06/20 09:43 AM (3 years, 9 months ago)

I don't see why I couldn't stick them in a quart jar and sterilize them in the PC. I doubt they would melt. Tho maybe there are parts in them that would. I will order some next time I get some money in my mycology budget and let ya'll know how it goes.


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OfflineA.k.aM
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Registered: 10/27/19
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Re: Agar transfer tool [Re: ahatchingegg]
    #26581344 - 04/06/20 10:00 AM (3 years, 9 months ago)

Demonic uses the punch too cuz he likes the perfect circles lol.

I also prefer scalpel, it took some getting used to but it’s nice to have the versatility once you get it down.


--------------------
LAGM2020


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,220
Last seen: 10 days, 8 hours
Trusted Cultivator
Re: Agar transfer tool [Re: A.k.a]
    #26581414 - 04/06/20 10:38 AM (3 years, 9 months ago)

I am using a real scalpel now, but when I started I used a nail cuticle tool. kinda flattened it out and then it was just 3 stabs to make the cut in the shape of a triangle and the transfer piece was easily scooped up and moved. Down side was how short the tool was so your hand is easy to get over top of your dish, scalpel puts me at a safe distance


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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Offlineahatchingegg
Stranger
Registered: 03/10/20
Posts: 71
Last seen: 9 months, 24 days
Re: Agar transfer tool [Re: Camera93]
    #26583442 - 04/07/20 08:23 AM (3 years, 9 months ago)

Based on the information ya'll have offered, I decided to go ahead and order some blunt tip luer lock needles instead of the biopsy punches. They are much less expensive and will allow me to take smaller samples for transfer.


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OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 29 days, 4 hours
Re: Agar transfer tool [Re: derek420]
    #26583464 - 04/07/20 08:40 AM (3 years, 9 months ago)

Quote:

derek420 said:
Those are pretty cool. But honestly you can do the same thing with a needle. Just poke the agar with the needle. You literally only need one cell of the myc and it’ll transfer and grow. Someone here has a tek on it but I can’t remember who.




https://www.shroomery.org/forums/showflat.php/Number/24740168


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinemetaphoric
Mr. Bater
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Registered: 05/18/15
Posts: 730
Last seen: 2 years, 2 months
Re: Agar transfer tool [Re: The Mycologist]
    #26583474 - 04/07/20 08:46 AM (3 years, 9 months ago)

Leather punch like d3monic uses is just like that except much cheaper. I'm definitely getting one when I cam afford it, I love those perfect little circles!


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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Offlineahatchingegg
Stranger
Registered: 03/10/20
Posts: 71
Last seen: 9 months, 24 days
Re: Agar transfer tool [Re: The Mycologist]
    #26583650 - 04/07/20 10:20 AM (3 years, 9 months ago)

I gotta bookmark that one!!


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Offlinemetaphoric
Mr. Bater
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Registered: 05/18/15
Posts: 730
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Re: Agar transfer tool [Re: ahatchingegg]
    #26583893 - 04/07/20 12:14 PM (3 years, 9 months ago)

His main thread is titled: D3 2020 Glog


--------------------
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:


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Invisiblemycosavant

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Registered: 02/18/20
Posts: 39
Re: Agar transfer tool [Re: ahatchingegg]
    #27143841 - 01/12/21 01:00 PM (3 years, 16 days ago)

Not really sure where else i would drop this, so here ya go.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5061704/


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☮ॐ
-नाद


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