Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
InvisibleALX8721
Male User Gallery

Registered: 03/06/19
Posts: 38
Water agar
    #26580336 - 04/05/20 07:44 PM (3 years, 10 months ago)

Anybody ever used agar and water to clean up a culture. Just agar and water? I recently heard of someone doing this and for the life of me can’t find it. I’d like to know the science or process behind it. TIA


--------------------
Just here to spread that love.


Extras: Filter Print Post Top
OfflineMugnath
Male


Registered: 05/24/12
Posts: 733
Loc: Oregon
Last seen: 9 months, 3 days
Re: Water agar [Re: ALX8721]
    #26580374 - 04/05/20 07:59 PM (3 years, 10 months ago)

Without any nutrients? I don't think it'll work.


Edited by Mugnath (04/05/20 08:00 PM)


Extras: Filter Print Post Top
InvisibleALX8721
Male User Gallery

Registered: 03/06/19
Posts: 38
Re: Water agar [Re: Mugnath]
    #26580534 - 04/05/20 09:14 PM (3 years, 10 months ago)

My thoughts exactly, and I know we can use an antibiotic agar. It sounds too basic to be true, but ya never know. I've seen people drop myc on poo (p. cyans) and skip the grain entirely here.


--------------------
Just here to spread that love.


Extras: Filter Print Post Top
InvisibleGeorge Sears
43rd President of the US
Male


Registered: 02/23/13
Posts: 1,477
Loc: New Mexico
Re: Water agar [Re: ALX8721] * 1
    #26580793 - 04/05/20 11:32 PM (3 years, 10 months ago)

Maybe grain soak water is what you saw? There needs to be some kind of nutrient added to the agar or else you're basically gonna have non-nutritive gelatin instead of anything useful.


--------------------


Edited by George Sears (04/05/20 11:33 PM)


Extras: Filter Print Post Top
InvisibleALX8721
Male User Gallery

Registered: 03/06/19
Posts: 38
Re: Water agar [Re: George Sears]
    #26580848 - 04/06/20 12:23 AM (3 years, 10 months ago)

would more than likely be from a transfer, the mycelium could use the nutes from original plate. Ive a buddy who swears it works, I'll post results.


--------------------
Just here to spread that love.


Extras: Filter Print Post Top
OfflineMachiavelliavore
Vermiculite Hater
 User Gallery


Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 4 months, 6 days
Re: Water agar [Re: ALX8721] * 1
    #26580865 - 04/06/20 12:43 AM (3 years, 10 months ago)

https://www.shroomery.org/forums/showflat.php/Number/19739087  Not sure if van used any karo in this experiment, but you'll prolly get something.  If you're dealing with bacteria that you can't get rid of, ive heard of people using antibiotics in there agar, if it's mold, usually you just need to transfer fast before the mold spores you transfer sporulate.  I don't know that there is much advantage to using no-nute agar for cleaning a culture though.  I think nutes will give more vigorous mycellial growth that will help you outpace mold sporulation when doing transfers.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 14 hours, 13 minutes
Trusted Cultivator
Re: Water agar [Re: Machiavelliavore]
    #26581365 - 04/06/20 10:13 AM (3 years, 10 months ago)

I think it’s called starving agar.


--------------------
LAGM2020


Extras: Filter Print Post Top
OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,233
Last seen: 1 hour, 21 minutes
Trusted Cultivator
Re: Water agar [Re: A.k.a] * 1
    #26581409 - 04/06/20 10:35 AM (3 years, 10 months ago)

shroomery facebook page with Alan talking a little about water agar and some comments that follow.


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



Extras: Filter Print Post Top
InvisibleALX8721
Male User Gallery

Registered: 03/06/19
Posts: 38
Re: Water agar [Re: Camera93]
    #26585324 - 04/08/20 12:13 AM (3 years, 10 months ago)

There it is, makes sense why I haven’t found anything. I’m not on FB. Thanks for the info!!!


--------------------
Just here to spread that love.


Extras: Filter Print Post Top
OfflineMmaven
Stranger
I'm a teapot
Registered: 07/02/19
Posts: 2
Last seen: 2 years, 1 month
Re: Water agar [Re: A.k.a]
    #27591969 - 12/23/21 02:35 PM (2 years, 1 month ago)

I know this is an older thread but I'm curious if you've seen this video on, in part, water agar.  Any thoughts?
[embed=640,360]https:/
/embed]


Extras: Filter Print Post Top
InvisibleDrboomer
The lord magnificent
I'm a teapot User Gallery

Registered: 09/22/19
Posts: 957
Re: Water agar [Re: ALX8721]
    #27591987 - 12/23/21 02:48 PM (2 years, 1 month ago)

I remember D3m talking about it


Extras: Filter Print Post Top
OfflinePsychdelicSpore
Funginner
Male

Registered: 08/08/22
Posts: 13
Last seen: 1 month, 22 days
Re: Water agar [Re: George Sears] * 1
    #27921636 - 08/27/22 05:55 PM (1 year, 5 months ago)

There is another thread on it. Basically, you can clean up mycelium by using WA to slow bacteria from growing faster than the mycelium. Helps to get a clean culture. There is a video on water Agar by "Fresh from the farm Fungi".


Extras: Filter Print Post Top
Onlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,842
Loc: In the Gills
Last seen: 6 minutes, 46 seconds
Re: Water agar [Re: PsychdelicSpore] * 1
    #27921731 - 08/27/22 07:11 PM (1 year, 5 months ago)



--------------------

Links For Beginners And Beyond:mspoil:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* G2G transfers...an endless cycle? ADoobie 7,207 18 11/12/20 07:10 PM
by FredsGotSlacks
* Agar plates, should I transfer yet?
( 1 2 all )
Albino Teacher 715 28 08/23/22 08:29 AM
by Albino Teacher
* Typically, how many agar transfers are required to achieve a clean mycelial sample? uninc4life2010 375 3 08/25/22 08:49 AM
by bakedbeings
* Is this healthy growth on my agar? HUBSonDUBS 1,278 7 01/23/07 04:48 PM
by HUBSonDUBS
* When to cut the agar? the_pigdog 829 9 09/10/06 05:50 AM
by ohmatic
* reduce contams on agar shymanta 1,155 6 02/07/07 03:25 PM
by shymanta
* Agar Work & Sterile Tek Questions - PC & Flame Sterilize + Antibotic Agar eltrkbrd 1,221 0 03/20/07 08:49 AM
by eltrkbrd
* agar question redshadow 460 4 01/27/06 09:22 AM
by Hengman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,481 topic views. 26 members, 108 guests and 31 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.006 seconds on 12 queries.