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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlineahatchingegg
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Registered: 03/10/20
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contaminated dishes
    #26577879 - 04/04/20 06:45 PM (3 years, 9 months ago)

hey all, just getting started with agar. I have a couple dishes thart are starting to look good. what should i do with dishes that are showing good myc growth but also some contamination? can i scrape up some of the good stuff to transfer or will it bring the contamination with it? do I need to do transfers as soon as I start seeing contamination to ensure that it's clean or can i transfer at any point?


Edited by ahatchingegg (04/04/20 06:45 PM)


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OfflineOvoidhunter
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Re: contaminated dishes [Re: ahatchingegg]
    #26577898 - 04/04/20 06:56 PM (3 years, 9 months ago)

You can still clean them up even tho they have contams. It might take a little longer and you may need a few more transfers but you can do it. The plate you have pictured should be alright to transfer. Also you asked if you should scrape it up.. You don't scrape it off the surface you cut a wedge out with a scapel or whatever you're using..


Your plate looks good I would transfer from it asap tho to lower chances of the contam carrying over. I would take transfers from around 5:30 try and cut small wedges too you don't need a big peice.


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Offlineahatchingegg
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Re: contaminated dishes [Re: Ovoidhunter]
    #26577903 - 04/04/20 06:59 PM (3 years, 9 months ago)

okay. i'll work on this today. thanks!


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OfflineUnknown error
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Re: contaminated dishes [Re: Ovoidhunter]
    #26577904 - 04/04/20 07:00 PM (3 years, 9 months ago)

Cut a small wedge from the leading edge of the good growth that’s away the the contamination and transfer to new plates.  Then repeat until you only have good even growth.


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Offlineahatchingegg
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Re: contaminated dishes [Re: Unknown error]
    #26579449 - 04/05/20 12:49 PM (3 years, 9 months ago)

Thanks ya'll. I made two transfers yesterday. What is better when I am done getting a clean culture? Transfer to liquid culture or right to grain? So far I have been doing MS to grain.


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Offlinepoisoned
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Re: contaminated dishes [Re: ahatchingegg]
    #26579487 - 04/05/20 01:09 PM (3 years, 9 months ago)

Go A2G first before trying LCs


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InvisibleRoger Clemency
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Re: contaminated dishes [Re: poisoned]
    #26579528 - 04/05/20 01:28 PM (3 years, 9 months ago)

Grain to grain (G2G) is a good way to expand your culture. So cut a wedge out and put it to a qt or pint of grain (pints will be done quicker) and when that’s ready you pour a bit of colonized grain into each of your sterilized qts of grain in the SAB.


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