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Tchatch
Stranger

Registered: 04/12/19
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Last seen: 3 years, 8 months
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Reviving a weak mushroom culture
#26577832 - 04/04/20 06:14 PM (3 years, 9 months ago) |
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Hi Everyone,
I've been cultivating mushrooms for a few years now and I have not yet stored cultures for long term storage in a proper way. I am pretty much just buying new cultures an keeping these up the longest I can until I have to buy new ones.
Anyways, this year my cultures were all stored as liquid cultures with a filter on jars for all winter. Some culture are still really viable and some are weak and grow really slow on grains. I guess the mycelium of these cultures has just gone through all the "food" there was in the liquid culture and could not grow any further, thus becoming weak. I know I would probably have been better to let these grow less long in the liquid culture before putting storing them in the fridge, but I guess I'll do better next time.
Anyways, here is my question then: Is there a way to get these weak cultures revived and performing well again and if there is, how should I do that?
I am currently wondering if these get gowing for a while and get some "food" and the mycelium "reproduces" itself, maybe it will do the trick? A short growing stage on agar would help? Let me know if you have tried anything that worked... If I can just get these to fruit and then use the spores from a mushroom, then I would be okay, but other than that?
Does a weak mycelium that has lacked food and weaken will get any better or is it just good for tossing away?
Edited by Tchatch (04/04/20 06:21 PM)
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rickyswamps
Bad Apple



Registered: 11/08/18
Posts: 1,192
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Re: Reviving a weak mushroom culture [Re: Tchatch] 1
#26577914 - 04/04/20 07:09 PM (3 years, 9 months ago) |
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Put them on agar and see how they do. They could of been nearly dead in the LC if its been that long.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Reviving a weak mushroom culture [Re: rickyswamps] 1
#26578727 - 04/05/20 04:45 AM (3 years, 9 months ago) |
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I just pulled mycellium from a 3-5 year old LC that was stored at room temperature. Didn't do much for 5-10 days, but eventually recovered on agar. Once it recovered it grew at an average rate. I have never really run into a slow old mycellium problem once it shows new growth. Any print you take from your grow should give rise to fresh new mycellium free of senescence.
I would definitely put those LC's on agar, and use fresh mycellial growth to noc your grains.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Tchatch
Stranger

Registered: 04/12/19
Posts: 3
Last seen: 3 years, 8 months
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I guess I'll try to put these on agar then. If it grows good, maybe put a wedge back back in LC to see how it does compared to the original slow growing LC.
I'll keep you in touch.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
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Re: Reviving a weak mushroom culture [Re: Tchatch]
#26580597 - 04/05/20 09:42 PM (3 years, 9 months ago) |
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Yeah, you can totally make a new LC out of a wedge with fresh mycellial growth.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Tchatch
Stranger

Registered: 04/12/19
Posts: 3
Last seen: 3 years, 8 months
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I have done some tests with the slow culture but it remains slow even after transfering it to agar. Transferring it to grain neither to agar seems to help the case.
I now have a new hypothesis...
The LC cultures wich are still very active and performant were initially inoculated from LC syringes. But the slow growing culture that I have was inoculated at first with a spore syringe.
Could it be that LC inoculated with spores is less resilient than say an LC with pure culture in it?
Let me know what you think..
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: Reviving a weak mushroom culture [Re: Tchatch]
#26626121 - 04/25/20 02:22 AM (3 years, 8 months ago) |
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Actually the culture that came out is fairly slow. I think it may have been slow to start with, honestly I can't remember. I got recovery off a 3-4 year old unrefrigerated plate growing much faster, but it was a different culture. I would have thought mycellium in water would do better than on agar at room temp, who knows. I'd just look for a print and work on getting something better. Certainly any syringe contaminants would not help, perhaps forcing the mycellium in the LC to expend energy to fight them off, though I would really think any contam in the syringe going into the LC would have been gg from day one.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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