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HoneyBadger9321
Mushroom Bookie



Registered: 08/07/18
Posts: 64
Last seen: 2 years, 7 months
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DIY Laminar Flow Hood Build by HoneyBadger9321 1
#26577124 - 04/04/20 11:49 AM (3 years, 9 months ago) |
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DIY Laminar Flow Hood Build or Tissue Culture Hood by HoneyBadger9321WHAT IS IT AND HOW IT WORKSA laminar flow cabinet or tissue culture hood is a carefully enclosed bench designed to prevent contamination of semiconductor wafers, biological samples, or any particle sensitive materials. Air is drawn through a HEPA filter and blown in a very smooth, laminar flow towards the user. Due to the direction of air flow, the sample is protected from the user but the user is not protected from the sample. The cabinet is usually made of stainless steel with no gaps or joints where spores might collect. Such hoods exist in both horizontal and vertical configurations, and there are many different types of cabinets with a variety of airflow patterns and acceptable uses. Laminar flow cabinets may have a UV-C germicidal lamp to sterilize the interior and contents before usage to prevent contamination of experiment. Germicidal lamps are usually kept on for 15 minutes to sterilize the interior and no contact is to be made with a laminar flow hood during this time. -Wikipedia Here is my humble laminar flow hood build, I've been planning this build for a while, but never got around to finishing it. With the current situation in my country and around the world with COVID-19. It certainly gave us a lot to think about and a lot of free time. So here it is (not yet finished, but I'd say 90% there, working platform and light missing): RESERCHI suggest you do your reserch before getting into building a LFH. They sure are expansive and require a lot of handy-work. If you're a begginer Id suggest you stick with a SAB, they are quite inexpensive and work like a charm. Floow hoods are unnecessary for mushroom cultivation, however they are fun as shit  . I decided to build mine for agar work, my SAB was quite clumbsy. First I gathered all the information I could on Shroomery and YouTube about LFH. Gathering info and understanding how it all works is key to designing/building anything. Most helpful and and intriging build, would be c10's: https://www.shroomery.org/forums/showflat.php/Number/24201748. Gathering the supplies and parts is next. There are 4 things you need to focus on: HEPA 14H 99.995% filter, pre-filter, centrifugal fan/blower and the fan/blower dimmer. Ill list down below where I got all my stuff, but let me save you some time. Since a LFH is quite expansive I looked for the cheapest options, you will see very reasonable/cheap options on sites like Aliexpress and Alibaba (from China), both for the filters and fan equipment. However there is a big disappointment, I've spent days! communicating with different vendors in the end they all offered the same thing, if I purchased just one item the postage was overpriced almost always over 150USD, just for postage! Save yourself some time and LOOK LOCALY FOR THE ITEMS. TO BUY LIST-HEPA H14 99.995% filter (99.995% is the minimum) 110€ -Pre-filter 20€ -Centrifugal fan/blower & dimmer 110€ -Beech plywood 50€ -White wood paint 10€ -Transparent silicone 8€ -Wood glue 5€ -Screws, nuts and bolts 5€ All together 318€ = 343USD
I'm from Europe so prices will differ from country to country. I got everything locally, except the blower that I got on ebay: https://www.ebay.co.uk/itm/Centrifugal-Centrifugal-Fan-Radial-Fan-1950m-H-5A-Speed-Governor-Adapter/153600927524?ssPageName=STRK%3AMEBIDX%3AIT&_trksid=p2057872.m2749.l2649, it came with a dimmer, big + since the correct dimmers are 40€.

DESIGNING THE BUILD If you know any 3D programs to sketch out the design and measurements is a plus. I use Rhino 3D modeling program. You can adjust the design to the equipment you have (filters, blower). Go BIG, go small...personally I will be adding a working surface and a brass cover with enclose plexiglass and a light.
 I tried to go for a simple, yet functioning design. It defently works for me.
STEP BY STEP
1. STEP: CUT THE PLYWOOD Get your plywood in my case beech, looking back I would prefer birch wood. Since beech was pretty hard to saw and screw into + it's quite heavier then birch.
  
2. STEP: LABELING AND PAINTING Take out your cut wood and prepare the area for painting. I suggest you label every plank/piece of wood, so that you know what is what.
    Lay it all out and add the first layer of paint, I did 4 layers all together. Here's a little painting tip/trick, after its all dry go grab the lightest sanding paper and a damp cloth. Very lightly sand the paint surface and then wipe it off with a the cloth. Water will enter the sanded gaps, making it super smooth!
3. STEP: FRAMING THE LFH Is attaching the frame for the HEPA filter and the other inner wood parts.
  I glued all the wood in place and then added screws for support. Be very persistent on the 90 degree angle, a couple mm off and it shows.
4.STEP: MOUNTING THE BLOWER Mount the fan/blower, use nuts and bolts mine are M5. Make sure you use washers on both sides, I've also added spring washers to make it extra tight.


5. STEP: ADDING THE HEPA FILTER AND THE FRONT AND BACK PANELS Add your HEPA filter, make sure you clean all the dust and little wood particles form cutting and sending. Trust me you dont want that in your HEPA. The filter should slide right in, pretty tightly. The same goes for the Pre-filter. And glue and crew the front panel onto the face of the LFH.
  
6. STEP: CAULKING Saw some videos on Youtube of people caulking at the end. Witch is fine, but it just looks messy to me. Caulk as you go, I say.
  
7. STEP: WIRING Mount your dimmer and drill the holes for the cables (make sure to caulk them from the inside). Every blower is different to wire. Here is how mine is wired (EU cable color code).
  
8. STEP: ADD THE LID PANEL Make sure it's all working like it should be and add the lid panel. I've just caulked mine. I will add hinges and locks, when they arive (COVID-19 really messing my creative vibe )
THE FINISHED PRODUCT

TIPS AND TRICKS -Add rubber between the blower and wood, this will absorb some of the vibrations making it quieter
Cheers guys, all the best! 
*In my free time I design and build all kinds of equipment (I'm an industrial designer by trade). If you like the LFH so much, but can't be bothered to build one. Drop me a DM and we can talk pricing and design.
-------------------- “Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.” ― Bill Hicks
 
Edited by HoneyBadger9321 (04/05/20 05:47 AM)
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HoneyBadger9321
Mushroom Bookie



Registered: 08/07/18
Posts: 64
Last seen: 2 years, 7 months
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: HoneyBadger9321] 1
#26578792 - 04/05/20 06:07 AM (3 years, 9 months ago) |
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Does anybody know how to fix the gradient problem? I can't seem to get the gradient to work after STEP 5.
-------------------- “Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.” ― Bill Hicks
 
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magicschoolbus



Registered: 02/19/18
Posts: 160
Loc: Shroomery
Last seen: 8 months, 15 days
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: HoneyBadger9321]
#26578878 - 04/05/20 07:33 AM (3 years, 9 months ago) |
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What do you mean "the gradient" ?
edit: Oh you're talking about the CSS in your post... You don't have the "{gradient}" square bracket tags around anything past step 4
Otherwise nice build! The top part is a nice upgrade. I just throw my prefilter infront of the intake and it gets held tight against the blower.
I originally bought 3 of those prefilters you're using to cut back on my blowers power a little because I couldn't even keep a lighter lit a foot away from the filter. Did Nothing. Got recommendation to buy a mini HEPA filter+Carbon filter which was actually a replacement part for a tiny air purifier called LEVOIT LV-H126 https://www.amazon.com/gp/product/B07DVTR1H7/ref=ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1
If you look at my sig you can see the filter I'm talking about sitting flat to the left of my blower. I just didnt have it propped up for the pic
Edited by magicschoolbus (04/05/20 07:40 AM)
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shevanel
Gone til November


Registered: 01/27/08
Posts: 1,517
Last seen: 1 year, 11 months
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: magicschoolbus]
#26578885 - 04/05/20 07:44 AM (3 years, 9 months ago) |
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Nice work.
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HoneyBadger9321
Mushroom Bookie



Registered: 08/07/18
Posts: 64
Last seen: 2 years, 7 months
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: magicschoolbus]
#26579069 - 04/05/20 09:35 AM (3 years, 9 months ago) |
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Quote:
magicschoolbus said: What do you mean "the gradient" ?
edit: Oh you're talking about the CSS in your post... You don't have the "{gradient}" square bracket tags around anything past step 4
Otherwise nice build! The top part is a nice upgrade. I just throw my prefilter infront of the intake and it gets held tight against the blower.
I originally bought 3 of those prefilters you're using to cut back on my blowers power a little because I couldn't even keep a lighter lit a foot away from the filter. Did Nothing. Got recommendation to buy a mini HEPA filter+Carbon filter which was actually a replacement part for a tiny air purifier called LEVOIT LV-H126 https://www.amazon.com/gp/product/B07DVTR1H7/ref=ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1
If you look at my sig you can see the filter I'm talking about sitting flat to the left of my blower. I just didnt have it propped up for the pic
I tried the gradient like I did others and it didn't do anything. Will try again idk 
Yeah the filter is quite tough, but i works fine for me, this is my fan at 3/4 power.
-------------------- “Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.” ― Bill Hicks
 
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HoneyBadger9321
Mushroom Bookie



Registered: 08/07/18
Posts: 64
Last seen: 2 years, 7 months
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: shevanel]
#26584500 - 04/07/20 05:14 PM (3 years, 9 months ago) |
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Thx shevanel, it works like a charm
-------------------- “Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Heres Tom with the Weather.” ― Bill Hicks
 
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Litch


Registered: 01/23/18
Posts: 34
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: HoneyBadger9321]
#26956844 - 09/26/20 11:17 PM (3 years, 4 months ago) |
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Appreciate the recent write up. Tackling this project now, what exact filter are you running? No shops around here seem to carry any decently sized HEPA filters.
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ConcreteWaves



Registered: 01/08/13
Posts: 648
Loc: United States
Last seen: 1 year, 2 months
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: HoneyBadger9321]
#27321377 - 05/24/21 03:25 PM (2 years, 8 months ago) |
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Great write up, your flow hood turned out great! What was total build cost? Can you throw up links to your filters along with alternatives to the blower that would work with this build? I’m thinking of doing the same but would want the prefilter on the top to allow flexibility to relocate the flow hood in the future but might setup into a new space where I have to set it up against the left wall.
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baldur
Stranger

Registered: 06/30/21
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: ConcreteWaves]
#27711899 - 03/28/22 06:25 PM (1 year, 9 months ago) |
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Nice build!
Might it be possible to get links to the parts you bought? The blower and the filters mostly.
Cheers!
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cekoxa
Fpv pilot


Registered: 07/03/14
Posts: 145
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: HoneyBadger9321]
#27888404 - 08/03/22 03:42 PM (1 year, 5 months ago) |
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Sevgili avrupalı dostum yeterince oynadıysan sat birazda biz oynayalım :hsodeviç:
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 24 days, 19 hours
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: cekoxa]
#27888485 - 08/03/22 04:51 PM (1 year, 5 months ago) |
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Wow, you did that whole thing with a frickin black and decker handheld?! Impressive. I woulda gone table saw for easier straight cuts.
When I did mine I did use birch, just cause I love birch. It was pretty easy to work with too.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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HILLBILLY OUTLAW
Above And Beyond!



Registered: 04/21/22
Posts: 2,858
Loc: Luckenbach Texas
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: Forrester]
#27888490 - 08/03/22 04:54 PM (1 year, 5 months ago) |
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I’m loving the build. Very good woodwork on OPs part. Would love a hood but the prices scare me. I’m a pretty handy guy when it comes to building things. May give it a go soon.
--------------------
 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 TEAM SPREAD THE LOVE! Smellyhobbit said: Embarrassment and bashfulness are leeches on your ability to learn.
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chocolatier
Stranger
Registered: 11/17/23
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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: HoneyBadger9321]
#28546172 - 11/17/23 08:09 AM (2 months, 10 days ago) |
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Nice build! I’m doing one for a room with a low slanted ceiling. Do you know if this would work the same (air flow wise/I know I’ll need structural mods to support the blower)if the blower enclosure was on the bottom instead of the top?
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RockinRobot
Stranger


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Re: DIY Laminar Flow Hood Build by HoneyBadger9321 [Re: chocolatier]
#28546182 - 11/17/23 08:19 AM (2 months, 10 days ago) |
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Quote:
chocolatier said: Nice build! I’m doing one for a room with a low slanted ceiling. Do you know if this would work the same (air flow wise/I know I’ll need structural mods to support the blower)if the blower enclosure was on the bottom instead of the top?
last post over a year old? think you really going to get an answer. You already started 4 threads on the same subject. stop necroing old threads and wait for a reply to your threads.
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