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Fireneeb
Stranger
Registered: 10/28/18
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Last seen: 3 years, 8 months
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Starting to lose my god damn mind
#26576167 - 04/03/20 09:07 PM (3 years, 9 months ago) |
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Alright so I’ve been at this for a bit over a year now. Had some hiccups at the beginning but after a few months thought I had it down. But over the last 4 months my contams have been out of control. I use SAB, and usually have only been seeing green on my G2G transfers. My agar jars all colonize 100%, but I’m losing entire sets of jars to green, running about 50% contams on G2G. My last batch I made sure I transferred my plates at least 4 times before using to rule out hidden contams. Should I switch to LC? I’m at the end of my rope lol
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Starting to lose my god damn mind [Re: Fireneeb]
#26576174 - 04/03/20 09:11 PM (3 years, 9 months ago) |
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are your contams coming from around your colonized grain or spread out like a technique issue
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
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Re: Starting to lose my god damn mind [Re: Fireneeb]
#26576247 - 04/03/20 09:43 PM (3 years, 9 months ago) |
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What kind of grain are you using? What sort of PC cycle do you use?
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Shr00mEater
Strange


Registered: 10/17/18
Posts: 985
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Re: Starting to lose my god damn mind [Re: LogicaL Chaos] 1
#26576257 - 04/03/20 09:49 PM (3 years, 9 months ago) |
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Don’t know the answer, but here’s some troubleshooting to perform.
Leave a jar uninocculated and unopened.
If it goes green, it’s your grain prep/pc cycle,
If it is still good even after the g2g transfer jars go green....
Then, It’s your cleanliness or technique.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Starting to lose my god damn mind [Re: Shr00mEater] 1
#26576265 - 04/03/20 09:51 PM (3 years, 9 months ago) |
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if it's uninoculated and goes green it's your filter, pc's kill mold.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,333
Loc: The Inexpressible...
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Re: Starting to lose my god damn mind [Re: Shr00mEater]
#26576272 - 04/03/20 09:52 PM (3 years, 9 months ago) |
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Great advice 
Nothing like a like biological troubleshooting
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deadmandave
Slime


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Re: Starting to lose my god damn mind [Re: cronicr]
#26576320 - 04/03/20 10:23 PM (3 years, 9 months ago) |
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Quote:
cronicr said: if it's uninoculated and goes green it's your filter, pc's kill mold.
true but the pc cycle could be inadequate, not long enough or air failed to evacuate before pressure builds, etc.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Starting to lose my god damn mind [Re: deadmandave] 1
#26576329 - 04/03/20 10:27 PM (3 years, 9 months ago) |
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it would still kill molds, molds die off at 140ish so not even boiling temps, you would have to forget to turn the pc on for it to be at fault.( I literally mean that, to pasturize I use my pc and heat it for five minutes and shut it off and my temps get up to 165)
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Fireneeb
Stranger
Registered: 10/28/18
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Last seen: 3 years, 8 months
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Re: Starting to lose my god damn mind [Re: cronicr]
#26576344 - 04/03/20 10:48 PM (3 years, 9 months ago) |
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My PC cycle is ok, I’ve left jars a few days already and no signs of contams. I was assuming it was a hidden contam in my agar until this last run where I took extra special care. So it must be my sterile technique, it’s just weird it ruins an entire batch every single time. I Pc 7 jars at a time and it will be all 7 go bad, but another 7 I do 15 min later are fine. So I was always assuming it was my master jar. I feel like I’ve watched franks g2g vid a million times so my technique was down but maybe it isn’t. Is LC still a worthy look at?
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Fireneeb
Stranger
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Re: Starting to lose my god damn mind [Re: cronicr] 1
#26576357 - 04/03/20 10:56 PM (3 years, 9 months ago) |
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It seems to be widespread, not from points of the grain. Must be sterile technique right? Times like this I think of principal skinner. “It’s not me that’s out of touch, it’s the mycelium that’s out of touch!”
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Starting to lose my god damn mind [Re: Fireneeb]
#26576364 - 04/03/20 11:05 PM (3 years, 9 months ago) |
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of is very worthy to look into, I like pastys ez lc! for g2g the key is quick steady movements , I like my receiving jars up on the left , have my lid rings off and lids loose, I open my master and start pouring from the far left working my way back towards the right never having my receiving jars open for more then a couple seconds
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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woofwoof
such mushrooms!



Registered: 01/04/19
Posts: 1,127
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Re: Starting to lose my god damn mind [Re: cronicr]
#26576586 - 04/04/20 02:06 AM (3 years, 9 months ago) |
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With your grain to grain receiving jars, unscrew the bands and lift with the polyfill to lift the lid. This way your not touching the rims or threads or anywhere on the receiving jars. If your lids are flipped (rubber side up) and you have polyfill as filter, then lifting the lid with polyfill will attach to the jar ring, thus removing a vector of contam to the game and also spending less time fucking around with bands and lids. Kill two birds in one stone
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
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Re: Starting to lose my god damn mind [Re: woofwoof] 1
#26576712 - 04/04/20 05:41 AM (3 years, 9 months ago) |
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This is why when I started with grains I bought a nice flowhood no matter the cost. I hated using an SAB and was the last variable in my equation. Once I got a flowhood I had an instant %99.99 success rate. I haven't seen one of my jars contaminate in 4yrs...
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Fireneeb
Stranger
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Re: Starting to lose my god damn mind [Re: rumfor69] 1
#26640014 - 04/30/20 09:44 PM (3 years, 8 months ago) |
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So a couple cycles later, still running about 50%. I did a couple test jars to troubleshoot, and I’m deff thinking it’s my technique. Left one without noccing it up (but still opened the jar in the SAB) and that batch all went bad except for that one. So I guess I’ve pinpointed where it is I’m going wrong. I’ve been a lot more diligent lately, and making sure I wipe down the top/under lid parts of my agar jars but still having issues. Does anyone have a video they could point me to for g2g sterile tek? It’s embarrassing I even have to ask that considering how long I’ve been at it. And I’m using mostly plastic lids, sometimes metal with SFDs. I’ve stayed away from polyfil
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Starting to lose my god damn mind [Re: Fireneeb]
#26640020 - 04/30/20 09:48 PM (3 years, 8 months ago) |
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Your culture could be comprimised too. I had this issue in my LAGM grow. Traced it back to my plates that showed fine but would all go green if taken from that culture. New culture no problems.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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