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OfflineBbaron
Stranger

Registered: 08/12/15
Posts: 51
Last seen: 2 years, 11 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: staytrippy420]
    #26573262 - 04/02/20 12:54 PM (3 years, 11 months ago)

Reishi from a culture syringe





Cleaned up with 1 transfer



Need to do a few more transfers as we got an intruder on this one


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OfflineBirdSeedUndaUrFeet
Micropope
I'm a teapot User Gallery


Registered: 10/29/18
Posts: 248
Loc: Germany Flag
Last seen: 3 years, 5 months
Re: AGAR ENVY! (Anything and All things agar!) [Re: Bbaron]
    #26573493 - 04/02/20 03:02 PM (3 years, 11 months ago)

Colombian Rust Spore inoculation plate, going to do some transfers soon:



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InvisibleD3_Myc
Weeb Trash
I'm a teapot


Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
Trusted Cultivator
Re: AGAR ENVY! (Anything and All things agar!) [Re: BirdSeedUndaUrFeet]
    #26573517 - 04/02/20 03:17 PM (3 years, 11 months ago)

Not really pretty agar but some my first pins


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InvisibleWhyterye
Male


Registered: 02/23/18
Posts: 1,226
Loc: Colorado Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26574055 - 04/02/20 09:22 PM (3 years, 11 months ago)

:rasta:

Edited by Whyterye (08/05/20 06:25 PM)

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OfflineEdmunter
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Registered: 05/01/13
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whyterye]
    #26574313 - 04/03/20 12:23 AM (3 years, 11 months ago)

Quote:

Whyterye said:
Is there any way to pour agar into sterilized jars or mini rounds ? Just don’t have any Petri dishes and I like the pour method .





I use a whiskey jar

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OfflineEdmunter
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter] * 1
    #26574440 - 04/03/20 03:01 AM (3 years, 11 months ago)

Agar porn

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Offlinedmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
    #26574874 - 04/03/20 09:45 AM (3 years, 11 months ago)

Quote:

Edmunter said:
Agar porn





hey Ed ;

so we know rhizo doesn't mean better ; you just look for vigor or speed ?  or are this proven performers ?


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...and when you loose control ; you'll reap the harvest you have sown.

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Offlinepoisoned
untitled
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Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
    #26574933 - 04/03/20 10:12 AM (3 years, 11 months ago)

:threadmonitor:


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How I do glass dishes

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Offlinetayson
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Registered: 01/04/18
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Re: AGAR ENVY! (Anything and All things agar!) [Re: poisoned]
    #26574961 - 04/03/20 10:23 AM (3 years, 11 months ago)

Tips for cloning agar pins? Ive been using a small pair of tweezers, need to invest in forceps. Curious if the myc at the base of the mushroom is from multiple sets of ms genetics? Does anyone ever remove the base or only take transfers when myc starts to grow from the center/cap instead of the base? Im probably over thinking it but I want to try removing the base from my next clones with sterilized scissors or something simple.


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I try.

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Invisiblestaytrippy420
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Registered: 03/23/13
Posts: 2,337
Loc: Canada Flag
Re: AGAR ENVY! (Anything and All things agar!) [Re: tayson]
    #26575015 - 04/03/20 10:45 AM (3 years, 11 months ago)

Your over thinking it. Flame your scalpel. Chop and drop it on a new plate.
If it’s from a germ plate most likely you’ll have to clean it up with transfers anyways.

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Invisiblestaytrippy420
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Registered: 03/23/13
Posts: 2,337
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Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
    #26575020 - 04/03/20 10:49 AM (3 years, 11 months ago)

Quote:

dmppb said:
Quote:

Edmunter said:
Agar porn





hey Ed ;

so we know rhizo doesn't mean better ; you just look for vigor or speed ?  or are this proven performers ?





Those look like isolates he’s been cleaning up?
I’m curious as well. They sexy af. :awesomenod:


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Tek's I use

LAGM2020

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OfflineEdmunter
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Re: AGAR ENVY! (Anything and All things agar!) [Re: staytrippy420] * 1
    #26575167 - 04/03/20 12:06 PM (3 years, 11 months ago)

They are all 2-3 transfers from the clones or pins.  I like to leave a few plates to age and pop up pins, these usually smash the pasty.  Not isolates but some are oins from clones 3 transfers in.  Isolation is narrowing those genetics down

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OfflineEdmunter
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
    #26575171 - 04/03/20 12:08 PM (3 years, 11 months ago)

By the way rhiz growth just looks nicer.  Any clean growth works.

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OfflineOvoidhunter
Buttery Crescent
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Registered: 09/17/16
Posts: 2,016
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
    #26575232 - 04/03/20 01:05 PM (3 years, 11 months ago)

Pretty sure this is mold. Smells minty.



These are from the same set of T1s so kinda sucks :mad:



Gonna make some new germ plates today and try again just in case. Gonna get the RW started.


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Offlinepoisoned
untitled
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Registered: 04/17/13
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ovoidhunter]
    #26575247 - 04/03/20 01:23 PM (3 years, 11 months ago)

I'd do a transfer from the transparent edge and do another smell test


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How I do glass dishes

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Offlinedmppb
On course


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ovoidhunter]
    #26575389 - 04/03/20 03:10 PM (3 years, 11 months ago)

Quote:

Ovoidhunter said:
Pretty sure this is mold. Smells minty.



These are from the same set of T1s so kinda sucks :mad:



Gonna make some new germ plates today and try again just in case. Gonna get the RW started.





i get that growth sometimes and it's usually just mycelium , it could start sectoring after that


--------------------
...and when you loose control ; you'll reap the harvest you have sown.

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Offlinemetaphoric
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Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
    #26575420 - 04/03/20 03:30 PM (3 years, 11 months ago)

Why do people cut such large chunks from their agar? That's not sarcastic, I'm genuinely asking. It seems to me like an empty pointed syringe would be the best tool for transfers. You just knick the surface with it and scoop an almost microscopic amount amount of myc. Keeps contams away and narrows genetics down to a more uniform set. 2 for 1, right? Or am I wrong? I'd love to hear what you think


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:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:
:mushroom:                  :mushroom2:                :mushroom:
:mushroom:            :mushroom2::mushroom2::mushroom2:            :mushroom:
:mushroom:      :mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:      :mushroom:
:mushroom::cookiemonster: BEHOLD :elmo::mushroom:
:mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom::mushroom:

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InvisibleLadysKnight
Hello Ladies
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Registered: 10/09/15
Posts: 1,852
Re: AGAR ENVY! (Anything and All things agar!) [Re: Ovoidhunter]
    #26575423 - 04/03/20 03:32 PM (3 years, 11 months ago)

Quote:

Ovoidhunter said:
Pretty sure this is mold. Smells minty.



These are from the same set of T1s so kinda sucks :mad:



Gonna make some new germ plates today and try again just in case. Gonna get the RW started.




Transfer a sample of just the outside edge of the clear shiny bit and see what grows. Maybe we can learn something.

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OnlineA.k.aM
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Registered: 10/27/19
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Re: AGAR ENVY! (Anything and All things agar!) [Re: metaphoric]
    #26575426 - 04/03/20 03:33 PM (3 years, 11 months ago)

Seems to usually be people just getting into agar and they have trouble handling smaller wedges during transfer.

Mine were never very big but as I got more experienced they definitely got smaller.


--------------------
LAGM2020

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Offlinepoisoned
untitled
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Registered: 04/17/13
Posts: 1,738
Loc: Yurop
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Re: AGAR ENVY! (Anything and All things agar!) [Re: metaphoric]
    #26575512 - 04/03/20 04:11 PM (3 years, 11 months ago)

Quote:

metaphoric said:
Why do people cut such large chunks from their agar? That's not sarcastic, I'm genuinely asking. It seems to me like an empty pointed syringe would be the best tool for transfers. You just knick the surface with it and scoop an almost microscopic amount amount of myc. Keeps contams away and narrows genetics down to a more uniform set. 2 for 1, right? Or am I wrong? I'd love to hear what you think



You can definitely just stick the syringe into one plate and then to another and you'll do a transfer. It just takes way longer for myc to recover and ain't nobody got time for that.


--------------------
How I do glass dishes

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