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trip_hazard
Lost in thunk



Registered: 08/29/18
Posts: 166
Loc: The happiest place on ear...
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First time playing with agar - so far, bupkis
#26575446 - 04/03/20 03:41 PM (3 years, 9 months ago) |
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After reading every link on the Agar Portal and getting my shit together I finally landed on Munchauzen's agar tech. I poured and PC'd a dozen containers and decided to try my hand at cleaning up two strains - PE from syringe and AA+ from spore. I did my best to follow clean procedure in my SAB and did the zig-zag swipe for both; that was on 3/30. I have checked on these buggers every day and so far I've got nada - no bacteria, no myc, no random contaminants, just nothing. Is it possible to screw up the 123 agar method so badly that I can't even contaminate a plate?

Edited by trip_hazard (04/03/20 06:26 PM)
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,328
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Re: First time playing with agar - so far, bupkis [Re: trip_hazard]
#26575493 - 04/03/20 04:02 PM (3 years, 9 months ago) |
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How long did u let the agar cool after PCing?
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trip_hazard
Lost in thunk



Registered: 08/29/18
Posts: 166
Loc: The happiest place on ear...
Last seen: 5 days, 2 hours
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Re: First time playing with agar - so far, bupkis [Re: LogicaL Chaos]
#26575501 - 04/03/20 04:04 PM (3 years, 9 months ago) |
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Two days - got busy and just left the PC on the stove closed for a couple days before I had a chance to noc'em up.
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: First time playing with agar - so far, bupkis [Re: trip_hazard]
#26576027 - 04/03/20 08:03 PM (3 years, 9 months ago) |
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spores can take up to two weeks to germinate. give it a few more days.
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brindle foxx
Doing it my way



Registered: 11/11/18
Posts: 420
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Re: First time playing with agar - so far, bupkis [Re: Munchauzen]
#26576037 - 04/03/20 08:05 PM (3 years, 9 months ago) |
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Quote:
Munchauzen said: spores can take up to two weeks to germinate. give it a few more days.
I seem to not be able to grow spores from print on Agar maybe I need to put them in a syringe solution. Tissue seems to be the only thing to work for me.. maybe i need to stop using premix PDA also I seem to let the agar cool until its chunky and the pour is ugly
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BibBonk
Stranger
Registered: 04/02/20
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Re: First time playing with agar - so far, bupkis [Re: trip_hazard]
#26576100 - 04/03/20 08:28 PM (3 years, 9 months ago) |
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Quote:
Is it possible to screw up the 123 agar method so badly that I can't even contaminate a plate?
I don't think so.
It's only been 4 days, that's not long enough to declare them failed.
If you want faster germination you can incubate them at 80F.
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trip_hazard
Lost in thunk



Registered: 08/29/18
Posts: 166
Loc: The happiest place on ear...
Last seen: 5 days, 2 hours
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Re: First time playing with agar - so far, bupkis [Re: Munchauzen]
#26576185 - 04/03/20 09:16 PM (3 years, 9 months ago) |
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Quote:
Munchauzen said: spores can take up to two weeks to germinate. give it a few more days.

Thanks - I think I've read too many threads where they're seeing partial coverage after a few days. What's been your longest duration to visual confirmation?
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trip_hazard
Lost in thunk



Registered: 08/29/18
Posts: 166
Loc: The happiest place on ear...
Last seen: 5 days, 2 hours
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Re: First time playing with agar - so far, bupkis [Re: BibBonk]
#26576210 - 04/03/20 09:28 PM (3 years, 9 months ago) |
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Quote:
BibBonk said:
Quote:
Is it possible to screw up the 123 agar method so badly that I can't even contaminate a plate?
I don't think so.
It's only been 4 days, that's not long enough to declare them failed.
If you want faster germination you can incubate them at 80F.
I've been out of the game for awhile - I'll submit it's possible that I've forgotten the first rule. Patience.
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