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Offlinemounsr
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Registered: 02/15/20
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Healthy or Unhealthy rye jars? What next?
    #26575261 - 04/03/20 01:35 PM (3 years, 10 months ago)

My first cube grow has been a bit rocky, and I'm hoping for advice on how to proceed with what I have. I inoculated rye jars with PE MS syringe on 2/23. Followed proper PC specs. Inoculated in a SAB using proper sterile technique. I believe a combo of incorrect lid tek, and living in a very cold/dry climate led to slow colonization. Also, likely contamination?

The following pics are the 3 jars furthest along in colonization.





I realize these 3 jars aren't very healthy, but I'd hate to abandon my first attempt. I shook them all at the recommended ~30% level, and they recovered well after a couple days. There appears to be continued growth each morning this week. But looking at other threads of healthy mycelium in rye jars, it's clear mine are struggling.

My initial intention was to spawn to bulk coir/verm. If a consensus can be reached that these jars are in better shape than I thought, I'll gladly wait for 100% and spawn to a monotub.

But if they are in fact FUBAR, what would be my best course of action to salvage this project as best I can, without spawning filthy substrate cakes and filling my house with contamination? I was thinking maybe spawn each jar to its own shoebox in my basement, far away from my designated "sterile" zone? Or should I spawn all jars to the same substrate and hope for the best? Or should I admit defeat, lick my wounds, and start over?

Thanks in advance for anyone's feedback. My box of 500 petri dishes arrived yesterday, and I'm looking forward to working with agar to avoid noob mistakes like this in the future. :thumbup:


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Offlinerumfor69
Bodhicitta Cultivator
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Registered: 08/05/11
Posts: 6,843
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Re: Healthy or Unhealthy rye jars? What next? [Re: mounsr]
    #26575306 - 04/03/20 02:14 PM (3 years, 10 months ago)

Those jars look pretty damn good to me. Definitely wait for them to
reach 100% colonization which I don't think will be a problem.

If those are your only 3 jars right now and you have agar then I
assume your goal is to clone some center flesh of a fruit to get
clean culture which is the way to go.

I'd recommend doing the 3 different shoeboxes probably so that way
there's three different experiments to achieve fruits from. Hopefully
some other feedback comes in here too.


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