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 Bbaron
Stranger
Registered: 08/12/15
Posts: 51
Last seen: 2 years, 29 days
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 Re: AGAR ENVY! (Anything and All things agar!)  [Re: staytrippy420]
#26573262 - 04/02/20 12:54 PM (4 years, 14 days ago) |
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Reishi from a culture syringe
Cleaned up with 1 transfer
Need to do a few more transfers as we got an intruder on this one
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BirdSeedUndaUrFeet
Micropope
 
Registered: 10/29/18
Posts: 248
Loc: Germany 
Last seen: 3 years, 6 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bbaron]
#26573493 - 04/02/20 03:02 PM (4 years, 14 days ago) |
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Colombian Rust Spore inoculation plate, going to do some transfers soon:
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 D3_Myc
Weeb Trash
Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Not really pretty agar but some my first pins
 
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Whyterye
Registered: 02/23/18
Posts: 1,230
Loc: Colorado 
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
#26574055 - 04/02/20 09:22 PM (4 years, 14 days ago) |
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Edited by Whyterye (08/05/20 06:25 PM)
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Edmunter
Mr
Registered: 05/01/13
Posts: 5,699
Last seen: 30 days, 2 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whyterye]
#26574313 - 04/03/20 12:23 AM (4 years, 14 days ago) |
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Quote:
Whyterye said:
Is there any way to pour agar into sterilized jars or mini rounds ? Just don’t have any Petri dishes and I like the pour method .
I use a whiskey jar
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Edmunter
Mr
Registered: 05/01/13
Posts: 5,699
Last seen: 30 days, 2 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter] 1
#26574440 - 04/03/20 03:01 AM (4 years, 14 days ago) |
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dmppb
On course
Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 1 month, 4 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
#26574874 - 04/03/20 09:45 AM (4 years, 13 days ago) |
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Quote:
Edmunter said:
Agar porn
      
hey Ed ;
so we know rhizo doesn't mean better ; you just look for vigor or speed ? or are this proven performers ?
--------------------
...and when you loose control ; you'll reap the harvest you have sown.
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poisoned
untitled
Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 5 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
#26574933 - 04/03/20 10:12 AM (4 years, 13 days ago) |
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tayson
ThatGuy
Registered: 01/04/18
Posts: 29
Loc: PNW
Last seen: 2 years, 8 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: poisoned]
#26574961 - 04/03/20 10:23 AM (4 years, 13 days ago) |
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Tips for cloning agar pins? Ive been using a small pair of tweezers, need to invest in forceps. Curious if the myc at the base of the mushroom is from multiple sets of ms genetics? Does anyone ever remove the base or only take transfers when myc starts to grow from the center/cap instead of the base? Im probably over thinking it but I want to try removing the base from my next clones with sterilized scissors or something simple.
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I try.
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staytrippy420
Registered: 03/23/13
Posts: 2,337
Loc: Canada 
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tayson]
#26575015 - 04/03/20 10:45 AM (4 years, 13 days ago) |
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Your over thinking it. Flame your scalpel. Chop and drop it on a new plate.
If it’s from a germ plate most likely you’ll have to clean it up with transfers anyways.
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staytrippy420
Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
#26575020 - 04/03/20 10:49 AM (4 years, 13 days ago) |
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Quote:
dmppb said:
Quote:
Edmunter said:
Agar porn
hey Ed ;
so we know rhizo doesn't mean better ; you just look for vigor or speed ? or are this proven performers ?
Those look like isolates he’s been cleaning up?
I’m curious as well. They sexy af. 
--------------------
Tek's I use
LAGM2020
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Edmunter
Mr
Registered: 05/01/13
Posts: 5,699
Last seen: 30 days, 2 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: staytrippy420] 1
#26575167 - 04/03/20 12:06 PM (4 years, 13 days ago) |
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They are all 2-3 transfers from the clones or pins. I like to leave a few plates to age and pop up pins, these usually smash the pasty. Not isolates but some are oins from clones 3 transfers in. Isolation is narrowing those genetics down
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Edmunter
Mr
Registered: 05/01/13
Posts: 5,699
Last seen: 30 days, 2 hours
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
#26575171 - 04/03/20 12:08 PM (4 years, 13 days ago) |
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By the way rhiz growth just looks nicer. Any clean growth works.
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Ovoidhunter
Buttery Crescent
Registered: 09/17/16
Posts: 2,016
Last seen: 2 years, 11 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Edmunter]
#26575232 - 04/03/20 01:05 PM (4 years, 13 days ago) |
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Pretty sure this is mold. Smells minty.
These are from the same set of T1s so kinda sucks 
 
Gonna make some new germ plates today and try again just in case. Gonna get the RW started.
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poisoned
untitled
Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 5 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ovoidhunter]
#26575247 - 04/03/20 01:23 PM (4 years, 13 days ago) |
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I'd do a transfer from the transparent edge and do another smell test
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dmppb
On course
Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 1 month, 4 days
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ovoidhunter]
#26575389 - 04/03/20 03:10 PM (4 years, 13 days ago) |
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Quote:
Ovoidhunter said:
Pretty sure this is mold. Smells minty.
These are from the same set of T1s so kinda sucks
Gonna make some new germ plates today and try again just in case. Gonna get the RW started.
i get that growth sometimes and it's usually just mycelium , it could start sectoring after that
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...and when you loose control ; you'll reap the harvest you have sown.
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metaphoric
Mr. Bater
Registered: 05/18/15
Posts: 730
Last seen: 2 years, 5 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: dmppb]
#26575420 - 04/03/20 03:30 PM (4 years, 13 days ago) |
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Why do people cut such large chunks from their agar? That's not sarcastic, I'm genuinely asking. It seems to me like an empty pointed syringe would be the best tool for transfers. You just knick the surface with it and scoop an almost microscopic amount amount of myc. Keeps contams away and narrows genetics down to a more uniform set. 2 for 1, right? Or am I wrong? I'd love to hear what you think
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 BEHOLD 
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LadysKnight
Hello Ladies
Registered: 10/09/15
Posts: 1,898
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Ovoidhunter]
#26575423 - 04/03/20 03:32 PM (4 years, 13 days ago) |
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Quote:
Ovoidhunter said:
Pretty sure this is mold. Smells minty.
These are from the same set of T1s so kinda sucks
Gonna make some new germ plates today and try again just in case. Gonna get the RW started.
Transfer a sample of just the outside edge of the clear shiny bit and see what grows. Maybe we can learn something.
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A.k.a
Stranger
Registered: 10/27/19
Posts: 16,965
Loc: Gaming the system
Last seen: 18 hours, 4 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: metaphoric]
#26575426 - 04/03/20 03:33 PM (4 years, 13 days ago) |
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Seems to usually be people just getting into agar and they have trouble handling smaller wedges during transfer.
Mine were never very big but as I got more experienced they definitely got smaller.
--------------------
 LAGM2020      
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poisoned
untitled
Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 1 year, 5 months
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Re: AGAR ENVY! (Anything and All things agar!) [Re: metaphoric]
#26575512 - 04/03/20 04:11 PM (4 years, 13 days ago) |
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Quote:
metaphoric said:
Why do people cut such large chunks from their agar? That's not sarcastic, I'm genuinely asking. It seems to me like an empty pointed syringe would be the best tool for transfers. You just knick the surface with it and scoop an almost microscopic amount amount of myc. Keeps contams away and narrows genetics down to a more uniform set. 2 for 1, right? Or am I wrong? I'd love to hear what you think
You can definitely just stick the syringe into one plate and then to another and you'll do a transfer. It just takes way longer for myc to recover and ain't nobody got time for that.
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