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Offlinecarnalrekt
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Brewers yeast is now producing psilocybin
    #26574385 - 04/03/20 03:42 AM (1 month, 24 days ago)



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OfflineInnerWisdom
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Re: Brewers yeast is now producing psilocybin [Re: carnalrekt]
    #26574406 - 04/03/20 04:07 AM (1 month, 24 days ago)

Omg, this is huge. Will definitely read that study. Wow.


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OfflineMikeTesserect
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Re: Brewers yeast is now producing psilocybin [Re: carnalrekt] * 1
    #26574420 - 04/03/20 04:28 AM (1 month, 24 days ago)

the writer needs to stop trying to be so fucking smart!


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OfflineInnerWisdom
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Re: Brewers yeast is now producing psilocybin [Re: MikeTesserect]
    #26574425 - 04/03/20 04:36 AM (1 month, 24 days ago)

"With the impending approval of psilocybin as a pharmaceutical drug, the manufacture and supply should be considered. Unfortunately, the content of psilocybin and psilocin in psychedelic mushrooms is too low (0.2%–1% dry weight) to make extraction a commercially viable option (Tylš et al., 2014), with cultivation of mushrooms for extraction of its psychedelic compounds limited to recreational users only. Furthermore, the large variation in psilocybin content observed between different mushroom batches will present difficulties in generating a consistent product with a stable supply chain needed for large scale therapeutic application "
What do you cultivators think of this? Seems kind of like bullshit to me that cultivation of mushrooms would not be a good method of obtaining psilocybin. On top of that, people could cultivate their own psilocybin, but that of course is something the drug companies don't want. Nevertheless, yeast capable of producing amounts of psilocybin like in this research of course yields much more substance in a much easier manner.


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Re: Brewers yeast is now producing psilocybin [Re: InnerWisdom]
    #26574683 - 04/03/20 09:27 AM (1 month, 24 days ago)

:popcorn:


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Re: Brewers yeast is now producing psilocybin [Re: tyrannicalrex] * 1
    #26574744 - 04/03/20 10:09 AM (1 month, 24 days ago)

What if you add msg to the brew & get an ethanol, GHB, psilocybin combo? :lol:


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OfflineQuirkmeister92
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Re: Brewers yeast is now producing psilocybin [Re: dizzy_simmons]
    #26578978 - 04/05/20 10:47 AM (1 month, 22 days ago)

Their yield is still <1%. I wonder how costly this process is. Probably gonna end up with the actual drugs being schedule 2 and mushrooms still illegal.


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OfflineInnerWisdom
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Re: Brewers yeast is now producing psilocybin [Re: Quirkmeister92]
    #26579052 - 04/05/20 11:27 AM (1 month, 22 days ago)

I am no expert, but yeast eats sugar so that part is very simple. The problem I guess is how to separate the bacteria from the psilocybin, or if that is even necessary.


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Invisibledizzy_simmons
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Re: Brewers yeast is now producing psilocybin [Re: InnerWisdom]
    #26580359 - 04/05/20 09:54 PM (1 month, 21 days ago)

Quote:

Quirkmeister92 said:
Their yield is still <1%. I wonder how costly this process is. Probably gonna end up with the actual drugs being schedule 2 and mushrooms still illegal.





While their yield is “only one percent” of the total solution, that’s still a fair bit of psilocybin. 
“A Johns Hopkins study found the ideal dose for long-term positive effects to be 20 mg per 70 kg of body weight.“ By that measure, their one liter has about 60 doses (including the psilocine, but not counting any other derivatives that might contribute to the effects of course). A “dose” would be less than 20mL, or 1% of a 2L bottle - less than a “shot” (30-40mL). 
HOWEVER, the silver lining is that in most places, mushrooms themselves were never scheduled (“made illegal”). They are usually considered containers of an illegal drug, therefore they bare the same il/legality as the substance they contain. Unless “they” completely rewrite the laws that is.

Quote:

InnerWisdom said:
I am no expert, but yeast eats sugar so that part is very simple. The problem I guess is how to separate the bacteria from the psilocybin, or if that is even necessary.




Yeast is actually a fungus, & small amounts of it are almost always present in beer & wine  :smile:
The FDA would definitely require a purified product, but I’d be more than happy with unrefined super brew.  Speaking of which, how can we get our hands on this strain?
I wonder if it was brewed aerobically or anaerobically.


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Onlinemusiclover420
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Re: Brewers yeast is now producing psilocybin [Re: InnerWisdom]
    #26581633 - 04/06/20 02:32 PM (1 month, 21 days ago)

Quote:

InnerWisdom said:

What do you cultivators think of this? Seems kind of like bullshit to me that cultivation of mushrooms would not be a good method of obtaining psilocybin. On top of that, people could cultivate their own psilocybin, but that of course is something the drug companies don't want. Nevertheless, yeast capable of producing amounts of psilocybin like in this research of course yields much more substance in a much easier manner.




Haven't even done mushrooms in awhile but still think it's BS. Some mushrooms get plenty potent, and even with under 1% yield mushrooms are easy to farm large scale and seem simple to extract.

They also bring up the variation in tryptamines in mushrooms as if it's a bad thing. But I'd wager a full spectrum blend with some variation could be more medicinal and effective then a pure psilocybin extract.


--------------------
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You know how it feels, With the breeze of the sun in your eyes. Not minding that time's passing by

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I talk to myself, I need nobody else. I'm lost and I'm mine, yes I'm free



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InvisibletyrannicalrexS
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Re: Brewers yeast is now producing psilocybin [Re: musiclover420]
    #26581646 - 04/06/20 02:38 PM (1 month, 21 days ago)

Wouldn't the 4-ho-(X) series of chems be like that?


--------------------

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OfflineShroomDoom
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Re: Brewers yeast is now producing psilocybin [Re: musiclover420] * 1
    #26581679 - 04/06/20 02:58 PM (1 month, 21 days ago)

Quote:

musiclover420 said:
Quote:

InnerWisdom said:

What do you cultivators think of this? Seems kind of like bullshit to me that cultivation of mushrooms would not be a good method of obtaining psilocybin. On top of that, people could cultivate their own psilocybin, but that of course is something the drug companies don't want. Nevertheless, yeast capable of producing amounts of psilocybin like in this research of course yields much more substance in a much easier manner.




Haven't even done mushrooms in awhile but still think it's BS. Some mushrooms get plenty potent, and even with under 1% yield mushrooms are easy to farm large scale and seem simple to extract.

They also bring up the variation in tryptamines in mushrooms as if it's a bad thing. But I'd wager a full spectrum blend with some variation could be more medicinal and effective then a pure psilocybin extract.



As much as I love cultivation of fruiting bodies this yeast liquid culture every 5 days yielding 275mg actives per liter is hard to beat on efficiency. All they did is controlled feed of glucose and oxygen bubbles for 5 days in a biofermenter then extract. Thats retardedly simple and way more efficient than fruiting psilocybin species in every way
It's simply faster on return, cheaper, rapidly scalable and can targeted for specific molecules. Can be most likely scaled to industrial size fermenters making hundreds of liters every 5 days, possibly less via optimized parameters. This will become the standard for commercially produced medical psilocybin, psilocin, baeocystin, norbaeocystin, and aeuriginascin. As far as home cultivation this may not be for masses because of the extraction step involved but it wouldn't be that hard for aspiring hobbyists who get their hands on the yeast to replicate production and extraction on a small scale. People will always grow mushrooms but this yeast makes active compounds more efficiently and with less time involved in the process.


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OfflineHolybullshit
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Re: Brewers yeast is now producing psilocybin [Re: musiclover420] * 1
    #26582371 - 04/06/20 08:17 PM (1 month, 20 days ago)

Quote:

musiclover420 said:
Haven't even done mushrooms in awhile but still think it's BS. Some mushrooms get plenty potent, and even with under 1% yield mushrooms are easy to farm large scale and seem simple to extract.

They also bring up the variation in tryptamines in mushrooms as if it's a bad thing. But I'd wager a full spectrum blend with some variation could be more medicinal and effective then a pure psilocybin extract.




It's about controlling the means of production. Although they make cloak it this way, it's got nothing to do with whether mushrooms produce enough psilocybin, it's just about controlling the supply and industrializing the process.

As far as what's "more medicinal", doesn't matter even if true, they aren't about to go through the trouble of trialing and bringing to market some kind of full spectrum extract or a drug consisting of multiple compounds...that would be a far more expensive and daunting task and there's no motivating factor for them to even try. You gotta think about patents and FDA approval.


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Offlinepolaritymind
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Re: Brewers yeast is now producing psilocybin [Re: Holybullshit] * 1
    #26583779 - 04/07/20 01:17 PM (1 month, 20 days ago)

Good luck trying to get your hands on this strain though, its gonna be a heavily guarded secret. As a previous poster said its about control.

Also I dont think this is news, it might have been bacteria last time but theres been an article before that someone has done this, its a pretty obvious and common move too since many drugs are mass produced this way trhough transgenic organisms nowadays like insulin, much cheaper than before.


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Invisibledizzy_simmons
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Re: Brewers yeast is now producing psilocybin [Re: polaritymind]
    #26584789 - 04/07/20 09:21 PM (1 month, 19 days ago)

Quote:

polaritymind said:

Also I dont think this is news, it might have been bacteria last time but theres been an article before that someone has done this, its a pretty obvious and common move too since many drugs are mass produced this way trhough transgenic organisms nowadays like insulin, much cheaper than before.




You're probably thinking of the GMO yeasts that could produce morphine; that story's floated around a couple times. Psilocybin isn't (yet) an accepted medicine, so there hasn't been much exploration with it particularly, 'though yeasts & bacteria are frequently modified.


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OfflineQuirkmeister92
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Re: Brewers yeast is now producing psilocybin [Re: dizzy_simmons] * 1
    #26585241 - 04/08/20 01:00 AM (1 month, 19 days ago)

The recent alternatives have require super expensive precursors to feed em. This is a GMO yeast, which they pretty much told you what to modify, and as ShroomDoom pointed out the turn around is unbelievably fast and yieldy. Psilocybin will be rescheduled sooner or later, but certainly not descheduled.


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Offlinesonoramo
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Re: Brewers yeast is now producing psilocybin [Re: polaritymind]
    #26586562 - 04/08/20 04:33 PM (1 month, 19 days ago)

Quote:

polaritymind said:
Good luck trying to get your hands on this strain though, its gonna be a heavily guarded secret...




I think we could count on a biochemist or two creating their own versions. The article describes a long list of strategies the authors used to produce the actives. If a copycat strain gets released with even half the potency, anybody who wants it can produce as much psilocybin as they ever wanted. Assuming the genetic modifications don't produce outright poisonous co-products, people might be brewing and drinking a small shot of something that tastes vaguely like mead for the equivalent of a 2-3g dose of dried Cubensis.

Something really interesting from the article is that the 600 mg/l concentration is extracellular. You don't even have to eat the yeast, just drink the brew it's growing in. And you really don't need to drink very much of it.

I wonder how stable the modified strain is. If the modification gives it no competitive benefit, the brew ought to eventually get contaminated by "normal" yeast.


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Re: Brewers yeast is now producing psilocybin [Re: sonoramo]
    #26586702 - 04/08/20 05:53 PM (1 month, 19 days ago)

“Control” hasn’t worked for mushrooms or GMO corn. I doubt it will work for this. With a little luck it’ll be available “for microscopy use only :shrug:


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OfflineHolybullshit
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Re: Brewers yeast is now producing psilocybin [Re: dizzy_simmons]
    #26586859 - 04/08/20 06:59 PM (1 month, 19 days ago)

Quote:

dizzy_simmons said:
“Control” hasn’t worked for mushrooms or GMO corn. I doubt it will work for this. With a little luck it’ll be available “for microscopy use only :shrug:




It will absolutely work for this, if psilocybin is brought to market as a prescription medicine you can bet your ass it will be sourced from yeast.

And about 90% of the soybeans and 70% of the cotton and corn grown in the US are roundup ready...and China just approved GMO corn now making Syngenta the largest corn seed supplier in Asia, so I'm not sure what you consider "working".


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Re: Brewers yeast is now producing psilocybin [Re: Holybullshit]
    #26587380 - 04/08/20 11:08 PM (1 month, 18 days ago)

Quote:

Holybullshit said:
Quote:

dizzy_simmons said:
“Control” hasn’t worked for mushrooms or GMO corn. I doubt it will work for this. With a little luck it’ll be available “for microscopy use only :shrug:




It will absolutely work for this, if psilocybin is brought to market as a prescription medicine you can bet your ass it will be sourced from yeast.

And about 90% of the soybeans and 70% of the cotton and corn grown in the US are roundup ready...and China just approved GMO corn now making Syngenta the largest corn seed supplier in Asia, so I'm not sure what you consider "working".




If by “control” you mean the method of production is so efficient someone would have to be brain dead not to use it as part of their business, then yeah, I’ll give you that hands down. But I can plant my own  corn right next to a plot of “sterile” GMO corn & “steal” those genes. Their “control” only prevents legal competition. It keeps the method out of the hands of other businesses, not the average (determined)  joe.


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OfflineHolybullshit
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Re: Brewers yeast is now producing psilocybin [Re: dizzy_simmons]
    #26591912 - 04/10/20 09:24 PM (1 month, 16 days ago)

By "control" I meant they can keep the production(and profits from) within the industry and not have to pay a mushroom farmer, the statement had nothing to do with the actions of others or clandestine production.

But since you are on the topic, this isn't something you can obtain just by getting "near" it(and chances are you would never be near it anyways, especially not in the same building with it). You'd have a much easier time obtaining bulk amounts of the psilocybin produced by said yeast than the yeast itself...do you generally find buying pure(before being formulated for market) schedule II drugs straight from the pharma producers easy? Or something that can be done through determination alone?

And I wouldn't recommend stealing GMO corn genetics. If you are growing it for profit you are opening yourself up to a lawsuit which will result in at least the loss of your crop if not much, much more...if you are just feeding yourself, is being able to spray your garden with pesticides really worth growing a dull generic strain ?



Edited by Holybullshit (04/10/20 09:40 PM)


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OfflineLewDoja
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Re: Brewers yeast is now producing psilocybin [Re: dizzy_simmons]
    #26600105 - 04/14/20 12:40 PM (1 month, 13 days ago)

Quote:

dizzy_simmons said:
What if you add msg to the brew & get an ethanol, GHB, psilocybin combo? :lol:



:whathesaid:


--------------------
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Invisibledizzy_simmons
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Re: Brewers yeast is now producing psilocybin [Re: Holybullshit]
    #26601170 - 04/14/20 07:53 PM (1 month, 13 days ago)

Quote:

Holybullshit said:
...do you generally find buying pure(before being formulated for market) schedule II drugs straight from the pharma producers easy? Or something that can be done through determination alone?




Straight from the pharmaceutical companies? No.
Straight from some guy in China who doesn't care about another country's drug laws or patents? I will neither confirm nor deny...

Quote:

Holybullshit said:
And I wouldn't recommend stealing GMO corn genetics. If you are growing it for profit...




I already addressed that, but I'm simply using GMO corn analogously. Yeast are microscopic organisms that lend themselves to being spread everywhere, intentionally or otherwise.


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Edited by dizzy_simmons (04/14/20 07:55 PM)


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Re: Brewers yeast is now producing psilocybin [Re: carnalrekt]
    #26608241 - 04/17/20 04:03 PM (1 month, 10 days ago)

lemme get my hands on some of that


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Re: Brewers yeast is now producing psilocybin [Re: dizzy_simmons]
    #26608734 - 04/17/20 08:14 PM (1 month, 9 days ago)

Quote:

dizzy_simmons said:
Quote:

Holybullshit said:
Quote:

dizzy_simmons said:
“Control” hasn’t worked for mushrooms or GMO corn. I doubt it will work for this. With a little luck it’ll be available “for microscopy use only :shrug:




It will absolutely work for this, if psilocybin is brought to market as a prescription medicine you can bet your ass it will be sourced from yeast.

And about 90% of the soybeans and 70% of the cotton and corn grown in the US are roundup ready...and China just approved GMO corn now making Syngenta the largest corn seed supplier in Asia, so I'm not sure what you consider "working".




If by “control” you mean the method of production is so efficient someone would have to be brain dead not to use it as part of their business, then yeah, I’ll give you that hands down. But I can plant my own  corn right next to a plot of “sterile” GMO corn & “steal” those genes. Their “control” only prevents legal competition. It keeps the method out of the hands of other businesses, not the average (determined)  joe.




They would certainly pasteurize the product before selling, so the only feasible way it would become public is if someone found a way to sneak it out of the lab...

Anyone want a temporary job going Undercover?
All applicants must cover their own legal cost in the event the lab finds out who dunnit.


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Re: Brewers yeast is now producing psilocybin [Re: Woodlandcarrot]
    #26611322 - 04/18/20 09:17 PM (1 month, 8 days ago)

what the...:eek:


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Offlinepolaritymind
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Re: Brewers yeast is now producing psilocybin [Re: meepins]
    #26622621 - 04/23/20 05:09 PM (1 month, 4 days ago)

Ok so in this duplicate news post a couple more interesting responses to this whole debate were added
https://www.shroomery.org/forums/showflat.php/Number/26612128

Quote:

This is a duplicate and likely to get closed, but I don't understand why people think this GMO strain is any more likely to get into the hands of the general public or be used for clandestine production any more than the strains that produce morphine.

Also, even if it did, I imagine that unless kept strictly isolated it would become contaminated with wild yeast and the inserted genes would be bred out fairly quickly.





And a discussion that the methods described might be copied, since theyre described in detail, by a clansestine biochemist. Also the discussion why cartels havent done this for opiod or opiod precursor producing yeast, which seems to exist also already.
So interesting stuff, check it out.

I also liked these

Quote:


dizzy_simmons said


Quote:

Holybullshit said:
And something tells me they didn't produce a modified strain of yeast with dominant genes allowing it to produce powerful hallucinogenic schedule I drugs...






Something tells me that isn't necessarily a factor we can 100% predict when using biochemical (I.e., biologically sourced) methods to splice & transfer genes. And again, we don't know whether this modification would make the yeast more or less fit. Psilocybin exists in other fungi for a reason, even if we don't know what that reason is.

Just my two cents.





Edited by polaritymind (04/23/20 05:14 PM)


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OfflineAz88
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Re: Brewers yeast is now producing psilocybin [Re: polaritymind]
    #26648791 - 05/04/20 07:57 PM (24 days, 3 minutes ago)

Yeast is an amazing organism


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InvisibletyrannicalrexS
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Re: Brewers yeast is now producing psilocybin [Re: Az88]
    #26650215 - 05/05/20 11:54 AM (23 days, 8 hours ago)

I have an amazing organism.:smirk:


--------------------

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