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Offlinemimir
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I have to make transfers, which would you choose?
    #26574013 - 04/02/20 08:59 PM (3 years, 9 months ago)


Here I have two first transfers from the result of germinating a spore print on agar. The one on the left looks strong but kinda weird, I'm afraid it might not be mycelium but rather some kind of mold. The one on the right doesn't look particularly exciting but looks like my clones, so is definitely cubensis mycelium. For some reason I've never gotten ryzo on agar, maybe due to poor GE that my glass petris provide. Anyway, which would you transfer from? I will probably transfer from both and then smell the left one to decide, but I'd like you guys' opinion. I should also mention that the agar stuck to my scalpel on the left one and wouldn't budge, so I kinda made a mess of it and might have brought in some contaminants. The black specks are burnt agar from previous transfers.


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InvisibleDmac316
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Re: I have to make transfers, which would you choose? [Re: mimir]
    #26574078 - 04/02/20 09:39 PM (3 years, 9 months ago)

As long as you have several plates ready for transfer I would suggest taking multiple from each and put on new plates and see what happens. I'm newer to agar as well and just now getting to T2 but of all the orginal plates and most T1 I've also noticed not alot of rhizomorphic growth except on just a couple. I'm pretty sure as you isolate you will see more rhizo but I am definitely not very experienced.


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InvisibleWhyterye
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Re: I have to make transfers, which would you choose? [Re: Dmac316]
    #26574127 - 04/02/20 10:16 PM (3 years, 9 months ago)

What agar recipe are you using ?


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Offlinemimir
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Registered: 01/21/19
Posts: 212
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Last seen: 13 days, 9 hours
Re: I have to make transfers, which would you choose? [Re: Whyterye]
    #26574743 - 04/03/20 08:08 AM (3 years, 9 months ago)

Quote:

Dmac316 said:
As long as you have several plates ready for transfer I would suggest taking multiple from each and put on new plates and see what happens. I'm newer to agar as well and just now getting to T2 but of all the orginal plates and most T1 I've also noticed not alot of rhizomorphic growth except on just a couple. I'm pretty sure as you isolate you will see more rhizo but I am definitely not very experienced.



That's what I'll end up doing eventually. I have had no luck even with isolates, this is what it looked like after a couple transfers:

It's ok though, that mycelium in the pic was from ms and gave me a ton of mushrooms when put to grains, so I'm not complaining.

Quote:

Whyterye said:
What agar recipe are you using ?



PDA. I boil the potatoes myself and make it slightly softer, especially when germinating spores.


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