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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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OfflineOne of Us
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Registered: 03/12/12
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First LC
    #26572658 - 04/02/20 06:19 AM (3 years, 10 months ago)

About 10 days ago, I inoculated my first LC using the josex biopsy method. I used 2% LME and I got crazy sediment... I never noticed it when making my 2% lme agar. My lc containers look like fricken ugly snow globes.  So, discouraged by all the sediment keeping me from seeing initial growth, I set the LCs aside and forgot about them, until now.

I now, at 10 days, have two LC containers with undisturbed, spherical ameba/jellyfish-looking growths.....on the bottom.... 

Is being on the bottom always a bad thing?  Honestly, I have a feeling they are contaminated, but I have zero lc experience. Any help, advice or input would be awesome.

Here are some pics. Keep in mind these are not cube mycelium, but are (supposed to be) pan and woodlover mycelium.





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Invisiblec10h12n2o
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Re: First LC [Re: One of Us]
    #26572666 - 04/02/20 06:32 AM (3 years, 10 months ago)

That's very weird looking

2% is way more than I would use for a lc, I use 1.5% for agar. I use 1.67g lme per liter of LC, sso that's less than 0.167%, less than 1/10th what you used. I always get perfect, fast, clear LCs at that nutrient level


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Re: First LC [Re: c10h12n2o]
    #26572687 - 04/02/20 07:02 AM (3 years, 10 months ago)

Well, I can say now that the top pictures are definitely mold. I took a look back at the culture I used and it appears to be sporulating...  along with all the other cultures I had from that species. I was cleaning mold the whole time! :facepalm: Unless anyone knows if caerulipes myc typically starts to turn tan after a 3-4 weeks (seems really slow for a mold, but it probably is).

Given how similar the other LC (pan) is looking/behaving, I am willing to bet that that too is mold.  Sound reasonable?


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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


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