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2Funguis
Stranger


Registered: 02/02/20
Posts: 13
Last seen: 3 years, 7 months
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Agar tips
#26571133 - 04/01/20 12:41 PM (3 years, 9 months ago) |
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I just made my first batch of 35 sugars dishes, I am overly excited and have been taking as many samples I possibly. I even got a few samples from a wild oyster mushroom I found, but my question is where do you guys take tissue samples from on yours cubensis for the best results? And how long will it take to see growth? Any other tips would also be greatly appreciated!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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tare your fruit in half and take an inner tissue sample
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Kmacmo
The aborted pin



Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 7 hours, 31 minutes
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For cubensis the inside of the stem is best bet, flesh that hasn't been exposed to open air and not near the cap you don't want any rogue spores on your clone samples. You'll see it starting to grow out within a few days, lower temps will slow things down.
There's no need to take lots of cuts from the same mushroom, just a couple will be enough you want to keep some plates so you can transfer them n clean it up
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WhiteWalker
Dr.



Registered: 04/28/19
Posts: 67
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Re: Agar tips [Re: Kmacmo]
#26571297 - 04/01/20 01:58 PM (3 years, 9 months ago) |
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As they said, just want to add some points. To be on safe page, you may get multiple cuts, yet do not waste your plates, you can use 3 different area on the same plate. They are all the same genetics so no problem doing that. If one is bad you can continue with others.
-------------------- "A diamond is a chunk of coal that did well under pressure." -Henry Kissinger
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2Funguis
Stranger


Registered: 02/02/20
Posts: 13
Last seen: 3 years, 7 months
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Re: Agar tips [Re: Kmacmo]
#26572176 - 04/01/20 09:52 PM (3 years, 9 months ago) |
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Thank you, and good call on the transfers I wasn't even thinking! So two more questions, how do you store your trays? And if the middle of my mushroom is basically hollow do I cut a piece off the inside wall?
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xnirvanax
Frank's biological son

Registered: 05/16/13
Posts: 131
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Quote:
2Funguis said: I just made my first batch of 35 sugars dishes, I am overly excited and have been taking as many samples I possibly. I even got a few samples from a wild oyster mushroom I found, but my question is where do you guys take tissue samples from on yours cubensis for the best results? And how long will it take to see growth? Any other tips would also be greatly appreciated!
Check out Stro's Cleaning and Isolating On Agar. Very useful information in this thread.Quote:
2Funguis said: Thank you, and good call on the transfers I wasn't even thinking! So two more questions, how do you store your trays? And if the middle of my mushroom is basically hollow do I cut a piece off the inside wall?
Get a scalpel or an x-acto knife and slice a piece of flesh off onto your agar dish.
Here is a thread by FrankHorrigan cloning a yellow oyster mushroom.
Store dishes in a zip-lock bag or on the shelf.
-------------------- Inspired by FrankHorrigan
Edited by xnirvanax (04/01/20 10:13 PM)
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