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OfflineTheLightGiver
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Registered: 02/27/17
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"Bush like" agar growth
    #26570559 - 04/01/20 07:39 AM (3 years, 9 months ago)

Hello everyone, after few years I finally decided to try growing again.
This time I started from spore prints I made 3 years ago on agar. Most of the plates germinated (all bacterial, but after few transfers I have some clean ones). But for some reason lot of the plates are growing really wierd, lot of growth from the transfered piece up, so much that in some cases the mycelium is starting to touch the lid hehe. Any ideas why is this happening? Thank you

Edit: It's Light malt extract agar (2g agar 2g LME/100ml water)




Edited by TheLightGiver (04/01/20 08:09 AM)


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Offlinerumfor69
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Re: "Bush like" agar growth [Re: TheLightGiver]
    #26570577 - 04/01/20 07:47 AM (3 years, 9 months ago)

Nice bush! 😉 But it kinda looks like good rhizo mycelium but
I'm curious what others think too


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Offlinemetaphoric
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Re: "Bush like" agar growth [Re: TheLightGiver]
    #26570580 - 04/01/20 07:49 AM (3 years, 9 months ago)

I'm eager to see what someone with more agar experience says but my money is on contam.


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Invisiblerickyswamps
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Registered: 11/08/18
Posts: 1,192
Re: "Bush like" agar growth [Re: metaphoric]
    #26570593 - 04/01/20 07:55 AM (3 years, 9 months ago)

Pic 3 has definite bacteria, the other plates look a little weird, but you'll have to wait a little longer to see.  What kind of agar recipe is that?


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OfflineTheLightGiver
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Re: "Bush like" agar growth [Re: rickyswamps]
    #26570603 - 04/01/20 08:04 AM (3 years, 9 months ago)

Sorry forgot to mention..It's Light malt extract agar (2g agar 2g LME/100ml water)

I know about the bacteria, will transfer soon. Just tried to pick the plates where this strange growth is most obvious. But most of my plates are showing similar growth to some degree.


Edited by TheLightGiver (04/01/20 08:04 AM)


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OfflineSynKyd
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Re: "Bush like" agar growth [Re: TheLightGiver]
    #26570636 - 04/01/20 08:25 AM (3 years, 9 months ago)

Maybe try another recipe? That growth doesn’t seem to want that agar. I have run LME and potato flakes side by sides and the results do vary kind of randomly with transfers from the same source.


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Edited by SynKyd (04/01/20 08:31 AM)


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Invisiblerickyswamps
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Registered: 11/08/18
Posts: 1,192
Re: "Bush like" agar growth [Re: SynKyd]
    #26570757 - 04/01/20 09:33 AM (3 years, 9 months ago)

LME agar is a good standard.  Tons of people use it and it works well.


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OfflineHamsabala
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Registered: 03/17/20
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Re: "Bush like" agar growth [Re: TheLightGiver]
    #26572485 - 04/02/20 02:21 AM (3 years, 9 months ago)

Do more transfers and you will see mycelium changes


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Invisiblexnirvanax
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Registered: 05/16/13
Posts: 131
Re: "Bush like" agar growth [Re: Hamsabala]
    #26572514 - 04/02/20 03:06 AM (3 years, 9 months ago)


:whathesaid:

The growth you are seeing is tomentose mycellium.




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OfflineTheLightGiver
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Registered: 02/27/17
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Re: "Bush like" agar growth [Re: rickyswamps]
    #26572904 - 04/02/20 09:38 AM (3 years, 9 months ago)

Oki, great, will keep on transfering :grin:

Thank you all very much  :bow2:


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