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OfflineKmacmo
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Registered: 08/14/19
Posts: 1,675
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Couple liquid culture questions, growth/needle size
    #26568977 - 03/31/20 12:10 PM (3 years, 9 months ago)

I've got this here jar inoculated a few weeks ago with a wedge of equdor mycelium T4, at what point should you put a lc into the fridge?

I have a few quarts of grain and a syringe full with water in the pc right now, the plan is to take it all into the SAB squirt out the water from the syringe then extract some LC solution and inoculate the three quarts of grain.

Its a pre used spore Syringe (disposable) I cleaned it good then filled with cold tap water wrapped in foil. Will the small needle size be an issue?


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OfflineOJ777
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Registered: 02/29/20
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Re: Couple liquid culture questions, growth/needle size [Re: Kmacmo]
    #26569000 - 03/31/20 12:18 PM (3 years, 9 months ago)

The needle size should be fine, just make sure that in both cases that your LC is at 100% for inoculation. For the jar that you threw the wedge into, store when it’s reached full colonization because growth within the fridge will be extremely slow. Hope that helps a little!


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OfflineKmacmo
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Registered: 08/14/19
Posts: 1,675
Loc: Central hemisphere
Last seen: 6 hours, 19 minutes
Re: Couple liquid culture questions, growth/needle size [Re: OJ777]
    #26569114 - 03/31/20 01:15 PM (3 years, 9 months ago)

Thanks for the info I was gonna use it tomorrow but its only about 80%full with mycelium.
Its all gathering  and going to the bottom, there's some clear liquid still left at the top


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