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OfflineMagic Mushpoon
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Registered: 03/24/19
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Inoculating with MS Plate?
    #26568777 - 03/31/20 10:27 AM (3 years, 9 months ago)

Hi Shroom people,

I have a 3 part question that I would really love some help with.

I just put together some bottle tek jars, they are cooling in the pressure cooker now. I stupidly did a bunch of transfers for my clones yesterday and now have very little to work with as far as colonized agar goes. My goal is to get make LI from one or more of the plates below and inoc 7 quart size pp5 jars. Here are my options.

1: I have some RustyWhyte MS plates that I was intending on forgetting about for a while until they form an invitro pin to clone. (I'm hyped for RWs) I know there is an increased risk of contams from inoculating with first transfer (or in this cased streaked) plates. They look clean to me, I'm fine with more risk as my options are limited. Would these work? Are there particular dishes that look better or worse? Are they at the right stage in colonization?

(don't know how to rotate these)


2. I have some old first transfers of iso's laying around too. These are plates that didn't look good enough or didn't grow fast enough for me to want to keep isolating them. I thought I'd let these invitro pin too. They're PESAs. I have a bunch of clones of PESAs in the works, so I'd rather use the RWs if possible. Would these be far superior to the MS plates? Are they too far along?


3. It's lockdown over here and I'm bored. My clones are going to be ready in a week, but I don't want to wait a week. With the two options of plates listed above, what are my options for projects? I want to get some shoeboxes going. I know people often go MS to shoeboxes, are they doing this with spore syringes or agar. If agar, do they just use streaked plates like mine above?

Thanks for all your help.


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Invisiblerickyswamps
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Registered: 11/08/18
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Re: Inoculating with MS Plate? [Re: Magic Mushpoon]
    #26568870 - 03/31/20 11:19 AM (3 years, 9 months ago)

There is no point of doing agar if you are going to inoculate with your germination plate.  You need to get at least 1 transfer away to know if its clean.  Might take 2-3 transfers to get it nice, just depends.  Some of those T1 plates look like they are possibly fine, hard to tell exactly from the photo.  They are a little too far grown out.


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OfflineMagic Mushpoon
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Re: Inoculating with MS Plate? [Re: rickyswamps]
    #26568885 - 03/31/20 11:29 AM (3 years, 9 months ago)

Thanks for the feedback. Unfortunately that's what I was expecting. So when people they grow MS on grain, they just do it with a syringe? Doesn't that seem equally ill advised?


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OfflineInfinity-25
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Re: Inoculating with MS Plate? [Re: Magic Mushpoon]
    #26568897 - 03/31/20 11:35 AM (3 years, 9 months ago)

By doing MS to agar without transferring like you're suggesting you only win some time compared to MS to grain directly, but equally high chances of contamination Id say as with MS to grain directly. Its slightly less ill advised therefore, but for sure better to do some transfers and make sure its clean.

Maybe you can take some transfers and knock up some jars with these after transferring to see what happens and get fruits sooner if you can spare the jars/space. Many people have succes going MS to grain so maybe worth a shot if you want to harvest sooner.

Just my 2 cents. Not very experienced myself yet but this is how I understand it.


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Offlinehazyhorse
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Re: Inoculating with MS Plate? [Re: Infinity-25]
    #26568913 - 03/31/20 11:44 AM (3 years, 9 months ago)

even if your earlier plates look clean, there’s a good chance they aren’t. i’ve gotten contams from second transfer plates that looked clean


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OfflineMagic Mushpoon
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Re: Inoculating with MS Plate? [Re: Infinity-25]
    #26568918 - 03/31/20 11:44 AM (3 years, 9 months ago)

Quote:

Infinity-25 said:
By doing MS to agar without transferring like you're suggesting you only win some time compared to MS to grain directly, but equally high chances of contamination Id say as with MS to grain directly. Its slightly less ill advised therefore, but for sure better to do some transfers and make sure its clean.

Maybe you can take some transfers and knock up some jars with these after transferring to see what happens and get fruits sooner if you can spare the jars/space. Many people have succes going MS to grain so maybe worth a shot if you want to harvest sooner.

Just my 2 cents. Not very experienced myself yet but this is how I understand it.



Thanks for this. It makes sense that the only benefit vs a syringe would be time saved.
Unfortunately, I have jars ready to be inoculated in the PC so I just don't have a lot of options.
As of now, I plan on PCing two seperate jars of water to make LI and use one plate of the MS and one plate of the overgrown iso. Worse case, I lose like $4 of supplies, 2 plates I wasn't going to do anything with and some time.

If people are interested to see if this works or not, I'd be happy to keep this thread updated. However its not like I'm trying anything too exciting here  :lol:


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OfflineMagic Mushpoon
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Re: Inoculating with MS Plate? [Re: Magic Mushpoon]
    #26568945 - 03/31/20 11:54 AM (3 years, 9 months ago)

I'm still unsure about how people generally go about growing from MS though. Unless I'm wrong I believe even experienced cultivators grow from MS, if they're looking for good clusters to clone, isolating varieties ect... I know they're not doing this with PF tek and I don't imagine they're knocking up grains with syrgines. What's the protocol for this?


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Invisiblerickyswamps
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Re: Inoculating with MS Plate? [Re: Magic Mushpoon]
    #26569168 - 03/31/20 01:45 PM (3 years, 9 months ago)

Put the spores on the plates, transfer clean wedges, when it looks clean, put it on grains.


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OfflineMagic Mushpoon
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Re: Inoculating with MS Plate? [Re: rickyswamps]
    #26569186 - 03/31/20 01:53 PM (3 years, 9 months ago)

Quote:

rickyswamps said:
Put the spores on the plates, transfer clean wedges, when it looks clean, put it on grains.



What's the difference between that and isolating strains?


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Invisiblerickyswamps
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Re: Inoculating with MS Plate? [Re: Magic Mushpoon] * 1
    #26569209 - 03/31/20 02:04 PM (3 years, 9 months ago)

Takes tons of transfers to isolate a strain.  You can clean up MS in 1-3 transfers and use it.  But its still many different strains.


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OfflineMagic Mushpoon
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Re: Inoculating with MS Plate? [Re: rickyswamps]
    #26569238 - 03/31/20 02:22 PM (3 years, 9 months ago)

Ahh makes sense. :thumbup:


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OfflineAtmozFear
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Re: Inoculating with MS Plate? [Re: rickyswamps]
    #26569288 - 03/31/20 02:41 PM (3 years, 9 months ago)

Quote:

rickyswamps said:
Takes tons of transfers to isolate a strain.  You can clean up MS in 1-3 transfers and use it.  But its still many different strains.



Thanks for that!


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