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Offlinedeathblade
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Sure wish I could grow less mold
    #26567898 - 03/30/20 06:22 PM (3 years, 9 months ago)

Ugh. I miss the old days when my tubs would be full of beautiful shrooms. I get little glimmers of hope occasionally but mostly just end up with green fuzzy as you can see in the pic. Cant seem to get it to stop.
I've included shots of my pastyplates with some new isolates. Started over from spores.  My only other thought is that I have mold mycelium hiding in the culture. Cause no matter how carefully I do my G2G and Agar transfers the majority of my tubs go green.

  Its unfortunate cause that culture used to produce well but I fear that over time it lost its ability to fight against contams.





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OfflinePOWAtrippinDiscord
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Re: Sure wish I could grow less mold [Re: deathblade]
    #26567918 - 03/30/20 06:37 PM (3 years, 9 months ago)

I challenge you to watch the intro of the 6 million dollar man, and get pumped to rebuild faster stronger etc


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OfflineeLeSDenes
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Re: Sure wish I could grow less mold [Re: POWAtrippin]
    #26567953 - 03/30/20 07:01 PM (3 years, 9 months ago)

start a new culture and the problem will go away


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InvisibleTranscendingLife
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Re: Sure wish I could grow less mold [Re: eLeSDenes]
    #26568084 - 03/30/20 08:26 PM (3 years, 9 months ago)

I don’t see mold in the fourth picture. :shrug:
Let your agar cultures grow out more before ditching them. You may get mold on half but the other half my be good to transfer.


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Re: Sure wish I could grow less mold [Re: TranscendingLife]
    #26568100 - 03/30/20 08:32 PM (3 years, 9 months ago)

Just went through the same thing. All issues were traceable through lineage to a single plate down the line. Just start over. You'll be happy 'fore you know it again.


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Re: Sure wish I could grow less mold [Re: TranscendingLife]
    #26568114 - 03/30/20 08:42 PM (3 years, 9 months ago)

Quote:

TranscendingLife said:
I don’t see mold in the fourth picture. :shrug:
Let your agar cultures grow out more before ditching them. You may get mold on half but the other half my be good to transfer.




:whathesaid:

I agree, fourth pic looks to be clean myc. Transfer when it gets about dime sized.


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Offlinedeathblade
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Re: Sure wish I could grow less mold [Re: TranscendingLife]
    #26568169 - 03/30/20 09:30 PM (3 years, 9 months ago)

Those are the new cultures. Unfortunately the old ones always look good to. I've wondered if it's my FH filter but when I've tested it by waving open plates in front of it they always stay clean. That's why I'm guessing theres something hiding on the culture.


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Offlinedeathblade
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Re: Sure wish I could grow less mold [Re: deathblade]
    #26568205 - 03/30/20 09:59 PM (3 years, 9 months ago)

I'm unsure of anything these days. I've been fighting this for so long now. A few little bursts of joy here and there but then absolutely nothing  but failures. Nothing makes sense.


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Offlinerumfor69
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Re: Sure wish I could grow less mold [Re: TranscendingLife]
    #26568322 - 03/30/20 11:59 PM (3 years, 9 months ago)

Quote:

TranscendingLife said:
I don’t see mold in the fourth picture. :shrug:
Let your agar cultures grow out more before ditching them. You may get mold on half but the other half my be good to transfer.



:whathesaid:


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Offlinedeathblade
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Re: Sure wish I could grow less mold [Re: rumfor69]
    #26568813 - 03/31/20 10:46 AM (3 years, 9 months ago)

Well I'm gonna try something this weekend. I usually mix up 6 tubs at a time. Gonna try 3 substrate mixes, 1 Hpoo with some verm and gypsum,1 coir/hpoo/verm and gypsum and 1 coir/verm/gypsum. If it's the substrate then something should work out.

  All jars will be stored inside totes as soon as they leave the PC and flowhood area. All cultures will be stored inside totes. I have been taking all transfers from 1 dish but moving forward will only take transfers from 1 plate per transfer. No more multiple plates from 1.

  I'm gonna make all subs drier. Maybe my field capacity is to wet. Dunno if this will fix anything but it's better than pulling my hair


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Offlinedeathblade
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Something completely different [Re: deathblade]
    #26604116 - 04/15/20 09:00 PM (3 years, 9 months ago)

Went to check on my master culture jars for all the new cultures I'm goin to try, and BAM couple of them are totally fucked. Noticeable green on the grains. So I figure that must have been contaminated Agar plates. So a week later I go to use 4 jars that I cooked up when I started my masters. And WTF theres mold growing inside these unopened and pressure cooked for 2 hours @15psi jars! I've had a few jars show green but not many out of hundreds. And never when I've left jars unopened. But I havent had jars sitting around not inoculated in forever.

Maybe this is the vector. I'm gonna go and buy grain from the whole foods store instead of the place I have been buying from for years.











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Offlinerumfor69
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Re: Something completely different [Re: deathblade]
    #26604635 - 04/16/20 01:55 AM (3 years, 9 months ago)

Hmmm and there's metal lid with little holes in it under that filter disk
it's not just the disk? Contaminated jars under a filter disk that were
never even opened...that's not gonna be the grain source it gotta be
the prep and cook procedure.

Soak 24hrs
Simmer for 10mins
Allow surface of grains to dry(toilet paper test)

How's your pressure cooker game? Jiggler weight definitely set for 15psi?
The weight barely jiggles as it cooks and you run it for 90mins?

Did you get your filter disks from a sponsor?


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Offlinepoisoned
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Re: Something completely different [Re: rumfor69]
    #26604777 - 04/16/20 04:00 AM (3 years, 9 months ago)

How do you cool down your PC?


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Offlinedeathblade
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Re: Something completely different [Re: poisoned]
    #26604876 - 04/16/20 05:22 AM (3 years, 9 months ago)

12hr soak. 10min boil. Then dry on a towel till ready to load into jars. 2 hr cook at 15psi
And yes metal lid with holes under the disc. My PC doesn't have a weight. Only a toggle. No the discs weren't from a sponsor but from a Canadian company.

After cooking I leave the cookers on the stove overnight to cool.


Edited by deathblade (04/16/20 05:24 AM)


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Offlinerumfor69
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Re: Something completely different [Re: deathblade]
    #26604888 - 04/16/20 05:34 AM (3 years, 9 months ago)

You're not releasing the pressure rapidly or anything are you?
Or does the cold cooker have a strong vaccum in the morning?


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Offlinedeathblade
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Re: Something completely different [Re: rumfor69]
    #26604934 - 04/16/20 06:10 AM (3 years, 9 months ago)

2 of the 3 have a vacuum. The other has a rubber emergency pressure relief plug that allows air inside after it cools. They're AA cookers.


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Offlinerumfor69
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Re: Something completely different [Re: deathblade]
    #26604952 - 04/16/20 06:18 AM (3 years, 9 months ago)

Those vacuums might be your problem. You should try to watch those 2
cool down and release whatever toggle thing that holds in pressure
when they get to 0 psi. Even if they have .5-1 psi in them still that
small rapid escaping air wont hurt the jars and will prevent the vacuum


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Offlinedeathblade
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Re: Something completely different [Re: rumfor69]
    #26604987 - 04/16/20 06:51 AM (3 years, 9 months ago)

I'm will to try anything to beat this green menace. I sure hope its as simple as releasing some vacuum from my cookers as they cool.


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Offlinerumfor69
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Re: Something completely different [Re: rumfor69]
    #26605869 - 04/16/20 01:28 PM (3 years, 9 months ago)

What happens is when they reach 0psi they start to create a vacuum as
they cool down beyond that point


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OfflineSockadin
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Re: Something completely different [Re: deathblade]
    #26605969 - 04/16/20 02:19 PM (3 years, 9 months ago)

Quote:

deathblade said:
Went to check on my master culture jars for all the new cultures I'm goin to try, and BAM couple of them are totally fucked. Noticeable green on the grains. So I figure that must have been contaminated Agar plates. So a week later I go to use 4 jars that I cooked up when I started my masters. And WTF theres mold growing inside these unopened and pressure cooked for 2 hours @15psi jars! I've had a few jars show green but not many out of hundreds. And never when I've left jars unopened. But I havent had jars sitting around not inoculated in forever.

Maybe this is the vector. I'm gonna go and buy grain from the whole foods store instead of the place I have been buying from for years.















Are you venting long enough? This is the only thing that could make properly sterilized jar go bad is that they are not properly sterilized.


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Offlinedeathblade
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Re: Something completely different [Re: Sockadin]
    #26606276 - 04/16/20 05:30 PM (3 years, 9 months ago)

Vent for 5 min before closing to build pressure.
Once again I'm confronted with something that just doesnt make sense.


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OnlineA.k.aM
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Re: Something completely different [Re: deathblade]
    #26606306 - 04/16/20 05:40 PM (3 years, 9 months ago)

It’s gotta be either the pressure cooker or bad filter.


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Offlinedeathblade
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Re: Something completely different [Re: A.k.a]
    #26606461 - 04/16/20 06:50 PM (3 years, 9 months ago)

It keeps getting worse. The filters look and feel, and were even packaged like the ones I got from FungiPerfecti. The mold in all the jars seems like it is on the surface suggesting that its falling on top. I feel like if it were the PC's fault it would be throughout the whole jar. Only the jar on the bottom right is still fine. Never opened and no mold showing. Theres enough jars now that its from 2 of the 3 cookers I run. But the remaining jars from the 2nd cooker look fine so far. I've been at this hobby for 14 years, I had no issues for 7 years and the last 7 it just keeps getting worse. I'm at a complete loss as to how to stop it or why it started. FML



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InvisibleSmartattack
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Re: Something completely different [Re: deathblade]
    #26606523 - 04/16/20 07:28 PM (3 years, 9 months ago)

Id bet you good money its all in your culture man. Its all the same contaminate, it not like you are getting a variety hour. All green.

Start a new culture and don't even use the same print or syringe, whichever you are starting with. I bet you beat it that way.


It also all looks like it is progressing from the vicinity of your agar drop. I don't see any distant contaminate growth starting in like some random corner of the jar, its all looking like it comes from your culture.


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Edited by Smartattack (04/16/20 07:31 PM)


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Offlinedeathblade
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Re: Something completely different [Re: Smartattack]
    #26606543 - 04/16/20 07:43 PM (3 years, 9 months ago)

Except a bunch of those jars have never been opened. And those are all fresh unique cultures.


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InvisibleSmartattack
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Re: Something completely different [Re: deathblade]
    #26606545 - 04/16/20 07:43 PM (3 years, 9 months ago)

oh.....I should read....lol.


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Re: Something completely different [Re: Smartattack] * 1
    #26606593 - 04/16/20 08:04 PM (3 years, 9 months ago)

I'm gonna argue the point . Some of the jars have foil on them.

You got an outbreak for sure.

Load, vent until the steam stops popping air. And then start your 2 hr cycle.

I bet that will fix it. Also get rid of those cellulose filter. Metal lid with some Micropore tape and a 1/4 hole works like a champ.


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Offlinedeathblade
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Re: Something completely different [Re: Sockadin]
    #26606702 - 04/16/20 08:50 PM (3 years, 9 months ago)

They're synthetic filter discs. I used them because of what I read on here. They sounded like the gold standard of filtration other than syringe filters.


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Offlinedeathblade
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Re: Something completely different [Re: deathblade]
    #26606707 - 04/16/20 08:53 PM (3 years, 9 months ago)

But I'm definitely willing to use micropore. Anything to stop the menace.


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Offlinerumfor69
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Re: Something completely different [Re: deathblade]
    #26606708 - 04/16/20 08:54 PM (3 years, 9 months ago)

I've only ever used synthetic filter disks they work perfectly
and if they're like the fungiperfecti ones they shouldn't be
the problem. And you already said you're venting the cooker first.

I'm still hanging with the cool down vacuum thing on the two
cookers


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InvisibleSmartattack
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Re: Something completely different [Re: deathblade]
    #26606710 - 04/16/20 08:54 PM (3 years, 9 months ago)

Out of hundreds of jars I never had a contam that could be traced to my simple holes stuffed with tight poly. I used 8 month old jars for spawn a few months ago. No issue, just a bit dry.


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Offlinedeathblade
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Re: Something completely different [Re: rumfor69]
    #26606778 - 04/16/20 09:22 PM (3 years, 9 months ago)

So here's a thought. I was wondering why sometimes I would have a couple tubs that didnt go green. Couldn't figure out what the difference was between them and the other 4 tubs that were mixed with the same spawn. 1 cooker holds enough jars for 2 tubs. I bet that the jars of spawn all lucked out and were from that 1 cooker with the rubber stopper. Maybe even the master jars occasionally. Good grief I hope thats it.


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Re: Something completely different [Re: deathblade]
    #26606792 - 04/16/20 09:28 PM (3 years, 9 months ago)

Now we need to see pictures of the cooker.

I have had cellulose filter disks do that because they didn't make a good seal. I cut all my SFD's down and RTV them to the metal lids.


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OnlineA.k.aM
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Re: Something completely different [Re: Sockadin]
    #26606856 - 04/16/20 09:57 PM (3 years, 9 months ago)

I literally just ran my first round of jars with sfds but I only rtvd a little circle on a couple, most I was in a hurry so just slapped the whole disk over the lid.

When you were having problems with whole disks were you still using the metal lid??
I’m assuming probably. I just hope I dont end up wasting a bunch of grain and plates cuz I didn’t want to wait on silicone to dry.


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Offlinedeathblade
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Re: Something completely different [Re: A.k.a]
    #26606877 - 04/16/20 10:10 PM (3 years, 9 months ago)

I used to cut the discs and silicone them down, but had issues with the silicone lifting a bit and allowing a pathway into the jars. So I used the ol search function and saw that other people had issues with cut discs. So I figured that instead of being cheap I would use a whole disc like many of the TC's. I've watched Roger Rabbits How to grow mushrooms must be a hundred times by now and he uses whole discs( I bought the dvd when it was first released and bought the downloadable version as well later). I've read grow lots and teks from at least a dozen TC's and copied their procedures to the best of my abilities.

And yet here we are with me being struck down mercilessly by an invisible enemy. Like I even built a new grow area. Perfectly dry, not an ideal location for any mold to grow. The only place that has any real moisture is inside my agar plates, jars, and tube.


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Offlinedeathblade
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Re: Something completely different [Re: deathblade]
    #26606881 - 04/16/20 10:13 PM (3 years, 9 months ago)

The one on the left has the rubber stopper.


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Offlinedeathblade
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Re: Something completely different [Re: deathblade]
    #26606904 - 04/16/20 10:30 PM (3 years, 9 months ago)

At the moment I'm conducting an experiment. I'm starting 6 tubs. 2 of straight poo and verm, 2 poo,coir,verm and 2 coir and verm.

I'm goin to take spawn jars at random from the 30 I have ready to go.

If all the tubs go green then I know it's the spawn.If only 4 go I will know it's the poo not being pasteurized properly and if only 2 go then it's my coir.

If the vacuum theory is correct then I still have at least 5 more jars that are gonna show me the green in the next couple days.


Edited by deathblade (04/16/20 10:32 PM)


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Offlinepoisoned
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Re: Something completely different [Re: deathblade]
    #26607038 - 04/17/20 12:19 AM (3 years, 9 months ago)

Dude, clean those pots


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Re: Something completely different [Re: Sockadin]
    #26607213 - 04/17/20 04:35 AM (3 years, 9 months ago)

All my mold and bacteria problems went away when i switched from no pour to petris, i fucked with nopours over a year and had like 2 semi good grows, i didnt know what to do so i bought media bottle, parafilm and 10 pcs of petris from ebay, from that day i only had green in 2-3 petris out of 50+ and all clean jars


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Re: Something completely different [Re: nix21] * 1
    #26607235 - 04/17/20 05:11 AM (3 years, 9 months ago)

Glass petri no pour is the real enlightenment tho :smile:


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Re: Something completely different [Re: nix21]
    #26607445 - 04/17/20 08:06 AM (3 years, 9 months ago)

I was thinking about goin back to pouring petri dishes. I started no pours cause I wanted to save some time. I still have a 3/4 case of petris.


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Re: Something completely different [Re: deathblade]
    #26665875 - 05/12/20 06:54 AM (3 years, 8 months ago)

So far my experiment has been a total failure lol. Everything seems to be going fine. The flush isn't very dense but at least its not moldy. I have no idea why these tubs are working and the last couple dozen have turned green. My process hasn't changed at all.
  I have a bunch of jars that I didn't use for tubs and 3/4 of them have shrooms trying to grow. No mold showing in any of those jars either.




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Re: Something completely different [Re: deathblade]
    #26780673 - 06/24/20 10:52 PM (3 years, 7 months ago)

Here's some pics of 3 new cultures. So far everything seems to be kool. Eliminated the coffee water grain soak before PC. (Used it for years but no more) And changed how I add water to my sub before pasteurization, by adding it to the coir and poo without the very seems to allow it to absorb moisture differently.  So now I'm adding quarter litre less.

I currently have 6 new cultures started and I am looking forward to what they do!



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Re: Something completely different [Re: deathblade]
    #26780677 - 06/24/20 10:55 PM (3 years, 7 months ago)

Those are wanting more fae. But good job nonetheless, I know I had a bad spell of nothing but mold showing up, now I have new cultures going as well.


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Offlinedeathblade
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Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
Re: Something completely different [Re: MH5109]
    #26780689 - 06/24/20 11:00 PM (3 years, 7 months ago)

Unfortunately when I give them more air it dries out to fast. 2 layers of micro pore seem to keep humidity high enough to allow growth. Ive been thinking about trying Pastywhytes style of mono without any tape just smaller holes.


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Offlinedeathblade
Long time lurker


Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
New beginnings [Re: deathblade]
    #27096404 - 12/18/20 06:48 PM (3 years, 1 month ago)

So scrapped the old culture. Isolated new strains and I'd say it looks good so far. 1 successful grow with the new culture and more on the way.
I'm not taking samples from only 1 petri now. Always multiple dishes. I'm also goin to continually do isolation so that if something happens to one culture I will have several others ready so that I dont have to work at it for half a year again.
New tub design, new no pour plate habits, and more agar work to keep things clean.



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