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Offlinedeathblade
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Registered: 06/25/06
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Re: Something completely different [Re: Sockadin]
    #26606276 - 04/16/20 05:30 PM (3 years, 9 months ago)

Vent for 5 min before closing to build pressure.
Once again I'm confronted with something that just doesnt make sense.


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OfflineA.k.aM
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Re: Something completely different [Re: deathblade]
    #26606306 - 04/16/20 05:40 PM (3 years, 9 months ago)

It’s gotta be either the pressure cooker or bad filter.


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LAGM2020


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Offlinedeathblade
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Re: Something completely different [Re: A.k.a]
    #26606461 - 04/16/20 06:50 PM (3 years, 9 months ago)

It keeps getting worse. The filters look and feel, and were even packaged like the ones I got from FungiPerfecti. The mold in all the jars seems like it is on the surface suggesting that its falling on top. I feel like if it were the PC's fault it would be throughout the whole jar. Only the jar on the bottom right is still fine. Never opened and no mold showing. Theres enough jars now that its from 2 of the 3 cookers I run. But the remaining jars from the 2nd cooker look fine so far. I've been at this hobby for 14 years, I had no issues for 7 years and the last 7 it just keeps getting worse. I'm at a complete loss as to how to stop it or why it started. FML



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InvisibleSmartattack
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Re: Something completely different [Re: deathblade]
    #26606523 - 04/16/20 07:28 PM (3 years, 9 months ago)

Id bet you good money its all in your culture man. Its all the same contaminate, it not like you are getting a variety hour. All green.

Start a new culture and don't even use the same print or syringe, whichever you are starting with. I bet you beat it that way.


It also all looks like it is progressing from the vicinity of your agar drop. I don't see any distant contaminate growth starting in like some random corner of the jar, its all looking like it comes from your culture.


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* Smarts videos :teacher:
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I'm a fungal white supremacist.


Edited by Smartattack (04/16/20 07:31 PM)


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Offlinedeathblade
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Re: Something completely different [Re: Smartattack]
    #26606543 - 04/16/20 07:43 PM (3 years, 9 months ago)

Except a bunch of those jars have never been opened. And those are all fresh unique cultures.


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InvisibleSmartattack
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Re: Something completely different [Re: deathblade]
    #26606545 - 04/16/20 07:43 PM (3 years, 9 months ago)

oh.....I should read....lol.


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* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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OfflineSockadin
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Re: Something completely different [Re: Smartattack] * 1
    #26606593 - 04/16/20 08:04 PM (3 years, 9 months ago)

I'm gonna argue the point . Some of the jars have foil on them.

You got an outbreak for sure.

Load, vent until the steam stops popping air. And then start your 2 hr cycle.

I bet that will fix it. Also get rid of those cellulose filter. Metal lid with some Micropore tape and a 1/4 hole works like a champ.


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Offlinedeathblade
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Re: Something completely different [Re: Sockadin]
    #26606702 - 04/16/20 08:50 PM (3 years, 9 months ago)

They're synthetic filter discs. I used them because of what I read on here. They sounded like the gold standard of filtration other than syringe filters.


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Offlinedeathblade
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Re: Something completely different [Re: deathblade]
    #26606707 - 04/16/20 08:53 PM (3 years, 9 months ago)

But I'm definitely willing to use micropore. Anything to stop the menace.


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Offlinerumfor69
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Re: Something completely different [Re: deathblade]
    #26606708 - 04/16/20 08:54 PM (3 years, 9 months ago)

I've only ever used synthetic filter disks they work perfectly
and if they're like the fungiperfecti ones they shouldn't be
the problem. And you already said you're venting the cooker first.

I'm still hanging with the cool down vacuum thing on the two
cookers


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InvisibleSmartattack
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Re: Something completely different [Re: deathblade]
    #26606710 - 04/16/20 08:54 PM (3 years, 9 months ago)

Out of hundreds of jars I never had a contam that could be traced to my simple holes stuffed with tight poly. I used 8 month old jars for spawn a few months ago. No issue, just a bit dry.


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* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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Offlinedeathblade
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Re: Something completely different [Re: rumfor69]
    #26606778 - 04/16/20 09:22 PM (3 years, 9 months ago)

So here's a thought. I was wondering why sometimes I would have a couple tubs that didnt go green. Couldn't figure out what the difference was between them and the other 4 tubs that were mixed with the same spawn. 1 cooker holds enough jars for 2 tubs. I bet that the jars of spawn all lucked out and were from that 1 cooker with the rubber stopper. Maybe even the master jars occasionally. Good grief I hope thats it.


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OfflineSockadin
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Re: Something completely different [Re: deathblade]
    #26606792 - 04/16/20 09:28 PM (3 years, 9 months ago)

Now we need to see pictures of the cooker.

I have had cellulose filter disks do that because they didn't make a good seal. I cut all my SFD's down and RTV them to the metal lids.


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OfflineA.k.aM
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Re: Something completely different [Re: Sockadin]
    #26606856 - 04/16/20 09:57 PM (3 years, 9 months ago)

I literally just ran my first round of jars with sfds but I only rtvd a little circle on a couple, most I was in a hurry so just slapped the whole disk over the lid.

When you were having problems with whole disks were you still using the metal lid??
I’m assuming probably. I just hope I dont end up wasting a bunch of grain and plates cuz I didn’t want to wait on silicone to dry.


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LAGM2020


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Offlinedeathblade
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Re: Something completely different [Re: A.k.a]
    #26606877 - 04/16/20 10:10 PM (3 years, 9 months ago)

I used to cut the discs and silicone them down, but had issues with the silicone lifting a bit and allowing a pathway into the jars. So I used the ol search function and saw that other people had issues with cut discs. So I figured that instead of being cheap I would use a whole disc like many of the TC's. I've watched Roger Rabbits How to grow mushrooms must be a hundred times by now and he uses whole discs( I bought the dvd when it was first released and bought the downloadable version as well later). I've read grow lots and teks from at least a dozen TC's and copied their procedures to the best of my abilities.

And yet here we are with me being struck down mercilessly by an invisible enemy. Like I even built a new grow area. Perfectly dry, not an ideal location for any mold to grow. The only place that has any real moisture is inside my agar plates, jars, and tube.


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Offlinedeathblade
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Re: Something completely different [Re: deathblade]
    #26606881 - 04/16/20 10:13 PM (3 years, 9 months ago)

The one on the left has the rubber stopper.


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Offlinedeathblade
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Re: Something completely different [Re: deathblade]
    #26606904 - 04/16/20 10:30 PM (3 years, 9 months ago)

At the moment I'm conducting an experiment. I'm starting 6 tubs. 2 of straight poo and verm, 2 poo,coir,verm and 2 coir and verm.

I'm goin to take spawn jars at random from the 30 I have ready to go.

If all the tubs go green then I know it's the spawn.If only 4 go I will know it's the poo not being pasteurized properly and if only 2 go then it's my coir.

If the vacuum theory is correct then I still have at least 5 more jars that are gonna show me the green in the next couple days.


Edited by deathblade (04/16/20 10:32 PM)


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Offlinepoisoned
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Registered: 04/17/13
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Re: Something completely different [Re: deathblade]
    #26607038 - 04/17/20 12:19 AM (3 years, 9 months ago)

Dude, clean those pots


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How I do glass dishes


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Invisiblenix21
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Re: Something completely different [Re: Sockadin]
    #26607213 - 04/17/20 04:35 AM (3 years, 9 months ago)

All my mold and bacteria problems went away when i switched from no pour to petris, i fucked with nopours over a year and had like 2 semi good grows, i didnt know what to do so i bought media bottle, parafilm and 10 pcs of petris from ebay, from that day i only had green in 2-3 petris out of 50+ and all clean jars


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Offlinepoisoned
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Re: Something completely different [Re: nix21] * 1
    #26607235 - 04/17/20 05:11 AM (3 years, 9 months ago)

Glass petri no pour is the real enlightenment tho :smile:


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How I do glass dishes


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