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deathblade
Long time lurker


Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
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Sure wish I could grow less mold
#26567898 - 03/30/20 06:22 PM (3 years, 9 months ago) |
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Ugh. I miss the old days when my tubs would be full of beautiful shrooms. I get little glimmers of hope occasionally but mostly just end up with green fuzzy as you can see in the pic. Cant seem to get it to stop. I've included shots of my pastyplates with some new isolates. Started over from spores. My only other thought is that I have mold mycelium hiding in the culture. Cause no matter how carefully I do my G2G and Agar transfers the majority of my tubs go green.
Its unfortunate cause that culture used to produce well but I fear that over time it lost its ability to fight against contams.
    
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POWAtrippin
Decidedly Undecided.



Registered: 10/22/08
Posts: 2,207
Loc: zone 9b
Last seen: 1 month, 19 days
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Re: Sure wish I could grow less mold [Re: deathblade]
#26567918 - 03/30/20 06:37 PM (3 years, 9 months ago) |
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I challenge you to watch the intro of the 6 million dollar man, and get pumped to rebuild faster stronger etc
-------------------- Don't believe everything you think. TRADE LIST ‹Sell Your Soul› You know this place is owned and operated by the Illuminati, right? ‹lsdwithme› i possibly just smoked a rat turd
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
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Re: Sure wish I could grow less mold [Re: POWAtrippin]
#26567953 - 03/30/20 07:01 PM (3 years, 9 months ago) |
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start a new culture and the problem will go away
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
Posts: 21,627
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Re: Sure wish I could grow less mold [Re: eLeSDenes]
#26568084 - 03/30/20 08:26 PM (3 years, 9 months ago) |
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I don’t see mold in the fourth picture.  Let your agar cultures grow out more before ditching them. You may get mold on half but the other half my be good to transfer.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Smartattack
C'mon man



Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Just went through the same thing. All issues were traceable through lineage to a single plate down the line. Just start over. You'll be happy 'fore you know it again.
-------------------- * Smarts videos * Planet of the APES   I'm a fungal white supremacist.
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HamHead
Hard Ass Motherfucker



Registered: 03/17/15
Posts: 6,107
Loc: Galactic sector ZZ9 Plura...
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Quote:
TranscendingLife said: I don’t see mold in the fourth picture.  Let your agar cultures grow out more before ditching them. You may get mold on half but the other half my be good to transfer.

I agree, fourth pic looks to be clean myc. Transfer when it gets about dime sized.
-------------------- The Italian researchers’ findings, published by the INT’s scientific magazine Tumori Journal, show 11.6% of 959 healthy volunteers enrolled in a lung cancer screening trial between September 2019 and March 2020 had developed coronavirus antibodies well before February. https://www.reuters.com/article/us-health-coronavirus-italy-timing-idUSKBN27V0KF This online first version has been peer-reviewed, accepted and edited, but not formatted and finalized with corrections from authors and proofreaders https://www.icandecide.org/
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deathblade
Long time lurker


Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
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Those are the new cultures. Unfortunately the old ones always look good to. I've wondered if it's my FH filter but when I've tested it by waving open plates in front of it they always stay clean. That's why I'm guessing theres something hiding on the culture.
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deathblade
Long time lurker


Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
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Re: Sure wish I could grow less mold [Re: deathblade]
#26568205 - 03/30/20 09:59 PM (3 years, 9 months ago) |
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I'm unsure of anything these days. I've been fighting this for so long now. A few little bursts of joy here and there but then absolutely nothing but failures. Nothing makes sense.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 21 minutes, 30 seconds
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Quote:
TranscendingLife said: I don’t see mold in the fourth picture.  Let your agar cultures grow out more before ditching them. You may get mold on half but the other half my be good to transfer.
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deathblade
Long time lurker


Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
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Re: Sure wish I could grow less mold [Re: rumfor69]
#26568813 - 03/31/20 10:46 AM (3 years, 9 months ago) |
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Well I'm gonna try something this weekend. I usually mix up 6 tubs at a time. Gonna try 3 substrate mixes, 1 Hpoo with some verm and gypsum,1 coir/hpoo/verm and gypsum and 1 coir/verm/gypsum. If it's the substrate then something should work out.
All jars will be stored inside totes as soon as they leave the PC and flowhood area. All cultures will be stored inside totes. I have been taking all transfers from 1 dish but moving forward will only take transfers from 1 plate per transfer. No more multiple plates from 1.
I'm gonna make all subs drier. Maybe my field capacity is to wet. Dunno if this will fix anything but it's better than pulling my hair
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deathblade
Long time lurker


Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
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Something completely different [Re: deathblade]
#26604116 - 04/15/20 09:00 PM (3 years, 9 months ago) |
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Went to check on my master culture jars for all the new cultures I'm goin to try, and BAM couple of them are totally fucked. Noticeable green on the grains. So I figure that must have been contaminated Agar plates. So a week later I go to use 4 jars that I cooked up when I started my masters. And WTF theres mold growing inside these unopened and pressure cooked for 2 hours @15psi jars! I've had a few jars show green but not many out of hundreds. And never when I've left jars unopened. But I havent had jars sitting around not inoculated in forever.
Maybe this is the vector. I'm gonna go and buy grain from the whole foods store instead of the place I have been buying from for years.





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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 21 minutes, 30 seconds
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Re: Something completely different [Re: deathblade]
#26604635 - 04/16/20 01:55 AM (3 years, 9 months ago) |
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Hmmm and there's metal lid with little holes in it under that filter disk it's not just the disk? Contaminated jars under a filter disk that were never even opened...that's not gonna be the grain source it gotta be the prep and cook procedure.
Soak 24hrs Simmer for 10mins Allow surface of grains to dry(toilet paper test)
How's your pressure cooker game? Jiggler weight definitely set for 15psi? The weight barely jiggles as it cooks and you run it for 90mins?
Did you get your filter disks from a sponsor?
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poisoned
untitled



Registered: 04/17/13
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Loc: Yurop
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Re: Something completely different [Re: rumfor69]
#26604777 - 04/16/20 04:00 AM (3 years, 9 months ago) |
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How do you cool down your PC?
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deathblade
Long time lurker


Registered: 06/25/06
Posts: 302
Last seen: 7 months, 22 days
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Re: Something completely different [Re: poisoned]
#26604876 - 04/16/20 05:22 AM (3 years, 9 months ago) |
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12hr soak. 10min boil. Then dry on a towel till ready to load into jars. 2 hr cook at 15psi And yes metal lid with holes under the disc. My PC doesn't have a weight. Only a toggle. No the discs weren't from a sponsor but from a Canadian company.
After cooking I leave the cookers on the stove overnight to cool.
Edited by deathblade (04/16/20 05:24 AM)
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
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Re: Something completely different [Re: deathblade]
#26604888 - 04/16/20 05:34 AM (3 years, 9 months ago) |
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You're not releasing the pressure rapidly or anything are you? Or does the cold cooker have a strong vaccum in the morning?
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deathblade
Long time lurker


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Re: Something completely different [Re: rumfor69]
#26604934 - 04/16/20 06:10 AM (3 years, 9 months ago) |
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2 of the 3 have a vacuum. The other has a rubber emergency pressure relief plug that allows air inside after it cools. They're AA cookers.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
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Re: Something completely different [Re: deathblade]
#26604952 - 04/16/20 06:18 AM (3 years, 9 months ago) |
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Those vacuums might be your problem. You should try to watch those 2 cool down and release whatever toggle thing that holds in pressure when they get to 0 psi. Even if they have .5-1 psi in them still that small rapid escaping air wont hurt the jars and will prevent the vacuum
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deathblade
Long time lurker


Registered: 06/25/06
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Re: Something completely different [Re: rumfor69]
#26604987 - 04/16/20 06:51 AM (3 years, 9 months ago) |
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I'm will to try anything to beat this green menace. I sure hope its as simple as releasing some vacuum from my cookers as they cool.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
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Last seen: 21 minutes, 30 seconds
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Re: Something completely different [Re: rumfor69]
#26605869 - 04/16/20 01:28 PM (3 years, 9 months ago) |
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What happens is when they reach 0psi they start to create a vacuum as they cool down beyond that point
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Sockadin



Registered: 01/03/10
Posts: 7,244
Last seen: 2 months, 20 days
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Re: Something completely different [Re: deathblade]
#26605969 - 04/16/20 02:19 PM (3 years, 9 months ago) |
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Quote:
deathblade said: Went to check on my master culture jars for all the new cultures I'm goin to try, and BAM couple of them are totally fucked. Noticeable green on the grains. So I figure that must have been contaminated Agar plates. So a week later I go to use 4 jars that I cooked up when I started my masters. And WTF theres mold growing inside these unopened and pressure cooked for 2 hours @15psi jars! I've had a few jars show green but not many out of hundreds. And never when I've left jars unopened. But I havent had jars sitting around not inoculated in forever.
Maybe this is the vector. I'm gonna go and buy grain from the whole foods store instead of the place I have been buying from for years.






Are you venting long enough? This is the only thing that could make properly sterilized jar go bad is that they are not properly sterilized.
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