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WestVirginaMan
The Omega

Registered: 09/23/18
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Loc: West Virginia
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Agar contamination help?
#26564475 - 03/29/20 03:24 AM (3 years, 10 months ago) |
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If someone would be kind enough to tell me if this looks like good mycelium or just some cobweb and a sad day.
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microdose83
Student



Registered: 12/31/19
Posts: 467
Last seen: 3 months, 25 days
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Looks to be tomentose mycelium. Let it go for a few more days and if it don't change color you should be good. Another way to tell at an early stage is to hold it up to the light. Most of the contams i run across in my experience look much darker through the light. Almost opaque compared to the mostly translucent mycelium. But I'm just about 3 months in to all this so I may be mistaken. Lol. Just my 2ยข. Here's some pics of tomentose (fluffy cottony) and rhizomorphic ( strands ropes) myc for a quick comparison to help.
-------------------- Trade List ๐ค๐๐ค Too weird to live, too rare to die!" ๐ค๐๐ค
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Re: Agar contamination help? [Re: microdose83]
#26564630 - 03/29/20 07:09 AM (3 years, 10 months ago) |
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A lot of times starting with spores on agar you'll see the fluffy mycelium first like that. Like he said let it get bigger see if it stay white and see through. Then do a transfer to another dish.
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WestVirginaMan
The Omega

Registered: 09/23/18
Posts: 68
Loc: West Virginia
Last seen: 3 years, 6 months
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Re: Agar contamination help? [Re: rumfor69]
#26565656 - 03/29/20 04:20 PM (3 years, 10 months ago) |
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Thank you both very much. Ill update pics in a day or two. This was the only way for me to start. I had a dried cap and tapped spores on two dishes and two i did a gill scrapping. I figured it was just contams but figured id ask since im brand new to this. Thanks for the help everyone. Ill post new better pics soon.
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WestVirginaMan
The Omega

Registered: 09/23/18
Posts: 68
Loc: West Virginia
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Ok so one of these 3 plates were the same one in the previous msg. I took the pics holding them up to the light after the mentioned translucent aspect. Not looking so good now. But one may be ok. But im asking. The last two pics are of the same dish.  
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WestVirginaMan
The Omega

Registered: 09/23/18
Posts: 68
Loc: West Virginia
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Funny thing is the one that may have survived is the one i cracked the plastic petri dish and had to tape it lol.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Those last two look good maybe. The first two it's hard to tell
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WestVirginaMan
The Omega

Registered: 09/23/18
Posts: 68
Loc: West Virginia
Last seen: 3 years, 6 months
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Re: Agar contamination help? [Re: rumfor69]
#26565768 - 03/29/20 05:28 PM (3 years, 10 months ago) |
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So basically i have 2 dried shrooms that i used to inoculate this agar. Looks like i ended up with one good plate to start jars or more agar. Could i use the same good plate for both jars and agar to keep it going? Just wondering the best way to go about this bc it may be my only chance. And so far looks like im 1/5 lol.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Think of making a big family tree or flow chart. This is the start, next is the first branches so you want to go wide so that these branches can make lots of little branches of their own. You can track everything with a series of numbers and letters. Branch 1 gen A, Branch 1 gen B, Branch 2 gen A, Branch 2 gen B, and so on. And I'd recommend making a bunch of prints outta the first branches or at least A gens of the different branches. Then all these different dishes will have different genetics to test and clone from. Preserve the beginning branches with long term slants. If a slant gets old you take it out, transfer it, reslant it and call it 1(1),1(2),1(3) and so on as the years go by
Something like this but not so scribbled quick
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WestVirginaMan
The Omega

Registered: 09/23/18
Posts: 68
Loc: West Virginia
Last seen: 3 years, 6 months
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Re: Agar contamination help? [Re: rumfor69]
#26566111 - 03/29/20 09:59 PM (3 years, 10 months ago) |
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So i should use the last 5 agar plates and use the one good non contaminated to inoculate the 5 new dishes. My problem is i dont have a pressure cooker. The best i can get is a "power pressure cooker xl" sadly lol. I think it only goes to 7.4 psi or something. My problem is the next step. I need to make spawn for the one good agar dish since my chances of getting more agar or another good dish is not looking good. So i know its basically impossible to make dpawn withoit a pc but im without any other option but to do so. I was wondering if i could do a coffe tek or a 3 day pasturization sterilization tek. But im looking for any help and as many ideas as i can get. Any way to keep this going. I appreciate the lc tek comment but looks like i need pc for it also. Unless someone has a lc tek that doesnt require a pc. Anyways thanks alot for all the help from everyone. Thats what makes this kinda work that much more enjoyable when there are ppl out there who care about the same thing. After trying these mushrooms the only thing i wanted to do after is grow more and spread the word. I just need some help from the wonderful community that has got me this far. Not to be corny that is lol.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
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Yeah a PC and a flowhood are the best two tools to have with this work.
Maybe someone will jump in here with some other advice on what to do without having a pressure cooker. Cause doing grains without one is probably gonna be almost impossible.
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WestVirginaMan
The Omega

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Loc: West Virginia
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Re: Agar contamination help? [Re: rumfor69]
#26566165 - 03/29/20 10:43 PM (3 years, 10 months ago) |
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I know. But thank you very much for your time. Im going ro continue this mission. Ive started with nothing and got this far, so with any luck and some help maybe this will end in something positive. Thanks again for your contribution. Maybe one day ill be posting good news.
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microdose83
Student



Registered: 12/31/19
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Last seen: 3 months, 25 days
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This may help you along the way without a pressure cooker. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=24981864&page=0&vc=1#24981864 And the pics of the agar, like rumfor69 said. The first 2 are hard to tell but the last 2 look pretty good.
-------------------- Trade List ๐ค๐๐ค Too weird to live, too rare to die!" ๐ค๐๐ค
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