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OfflineNinnyMuggins
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Containation? Not enough FAE? Something else?
    #26565358 - 03/29/20 01:56 PM (3 years, 9 months ago)

Hello All!

It's been a bit since I've been on but I've got a new batch cooking and I'd like to see about getting some opinions on it.

From looking at the contamination thread, it looks like I may be dealing with some Trich in some of my shoeboxes, but I wanted to see what you all think.
I also want to get some opinions about how to reduce the bottom pinning with my PE...it just loves to bottom pin!

And here's where I know I'm going o get a lot of grief...I've read the threads and the issues and how much everyone hates Martha Chambers but I have little choice in the mater since I have extremely limited space and need to keep things as inconspicuous as possible.
I have built a chamber that I control the amount of fresh air input and humidity and temp via an arduino and there are changes that I want to make to the overall design but it has been work ok for the most part.

I'd love it if you all could take a look at these and see if you think they need more FAE/Humidity/Whatever.

This is one of the GT boxes. In the top half of the photo you can see that there is a kind of white groth over the top that looks very similar to the Trich photo in the contamination thread:


Here's a photo of my most successful GT box...I think there is more of the same:

(I got a spore print and am currently in the process of trying to isolate the best genetics...I'll be cloning that big fella to agar soon)

This GT has the furry little tendrils which I think are areal mycelium which would indicate a need for more fae...I think:


Here's another GT; both photos are of the same box with the second focussed on what looks to be contamination to me. I isolated it away from the others since the one side looks strange and discolored. It does seem to have a lot of hyphal knots though right?:


Here's one of the PE boxes with some of the white globby growth sparsed throughout:


Here's another PE (second image is a close up of some of the blobby stuff::


Here's yet another PE which is super blobby looking:


Here's a couple of PE's that were bottom pinning like crazy so I dubtubbed them and raised them up so that the top pins could continue to grow as well:



What do you think? Should I increase airflow? decrease humidity? Throw them all into the incinerator?

As always thank you for any help and happy quarantining!

EDIT: Just reading a ton of threads about bottom pinning and I think the general consensus is my surface conditions aren't optimal. dialing in how often to cycle he fan in this grow chamber and how much humidity there should be is probably going to be the toughest part of all this.


Edited by NinnyMuggins (03/29/20 04:41 PM)


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