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InvisibleGrumio

Registered: 10/18/19
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How long can I keep cloning?
    #26564785 - 03/29/20 08:42 AM (3 years, 9 months ago)

So I've gotten into agar the last couple months, tried different ways, spore solution to agar, swab to agar, clone to agar and pin to agar.

Say if I grow one monotub, clone a fruit to agar and then agar to grain, can I clone a fruit from the fruit this tub grows and so on and so on or do I have to take prints and start from scratch again?

Thanks,


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InvisiblebodhisattaMDiscordReddit
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Re: How long can I keep cloning? [Re: Grumio] * 1
    #26564801 - 03/29/20 08:52 AM (3 years, 9 months ago)

You keep the original clone culture to start new grows. You don't keep cloning but you could do it until it doesn't work anymore which would probably take a very long time


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OfflineClc420
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Re: How long can I keep cloning? [Re: bodhisatta]
    #26617120 - 04/21/20 08:48 AM (3 years, 9 months ago)

Quote:

bodhisatta said:
You keep the original clone culture to start new grows. You don't keep cloning but you could do it until it doesn't work anymore which would probably take a very long time




How do you keep the original clone culture without isolating it? I would of thought you would end up isolating sectors after a few transfers


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Offlinepoisoned
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Re: How long can I keep cloning? [Re: Clc420]
    #26617143 - 04/21/20 08:55 AM (3 years, 9 months ago)

It's highly debatable if there's really multiple cultures present in one fruit. I don't think there's any evidence to support that claim.


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OfflineA.k.aM
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Re: How long can I keep cloning? [Re: poisoned]
    #26617155 - 04/21/20 09:03 AM (3 years, 9 months ago)

There definitely are from what I’ve seen.

This is the same clone, t3 and t1.



I was amazed how much the genetics narrowed from two transfers.


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OfflineClc420
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Re: How long can I keep cloning? [Re: A.k.a]
    #26617161 - 04/21/20 09:08 AM (3 years, 9 months ago)

Quote:

A.k.a said:
There definitely are from what I’ve seen.

This is the same clone, t3 and t1.



I was amazed how much the genetics narrowed from two transfers.





That's probably from narrowing down to sectors. Eventually your going to pick out an individual sector, that's my thoughts on it anyway. Nice looking tubs 🤘


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OfflineClc420
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Re: How long can I keep cloning? [Re: poisoned]
    #26617163 - 04/21/20 09:09 AM (3 years, 9 months ago)

Quote:

poisoned said:
It's highly debatable if there's really multiple cultures present in one fruit. I don't think there's any evidence to support that claim.




Theres loads of evidence lol. You can get multiple sub strains from a single fruit


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Offlinepoisoned
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Re: How long can I keep cloning? [Re: Clc420]
    #26617189 - 04/21/20 09:18 AM (3 years, 9 months ago)

Where's that evidence? I'd love to see it.


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OfflineA.k.aM
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Re: How long can I keep cloning? [Re: poisoned]
    #26617211 - 04/21/20 09:24 AM (3 years, 9 months ago)

It’s in the pictures I just put up.

Also myc from tissue samples will sector out on plates, which doesn’t happen with a single set of genetics.


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Offlinepoisoned
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Re: How long can I keep cloning? [Re: A.k.a]
    #26617231 - 04/21/20 09:30 AM (3 years, 9 months ago)

Could be different expression of the genes. Sectoring can appear on isolates tho. I'm now rescuing a commercial g. lucidum isolate and it definitely used to sector in the past when I was working with that culture.

I'm not saying it's not true, but it's definitely not a proven fact and is debatable.


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OfflineClc420
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Re: How long can I keep cloning? [Re: poisoned]
    #26617462 - 04/21/20 10:57 AM (3 years, 9 months ago)

Quote:

poisoned said:
Where's that evidence? I'd love to see it.



Quote:

poisoned said:
Where's that evidence? I'd love to see it.





Theres a thread which I think is linked in one of bods agar teks where the guy talks about cloning and then isolating cultures from the clones.


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InvisiblebodhisattaMDiscordReddit
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Re: How long can I keep cloning? [Re: poisoned] * 1
    #26617514 - 04/21/20 11:18 AM (3 years, 9 months ago)

Quote:

poisoned said:
It's highly debatable if there's really multiple cultures present in one fruit. I don't think there's any evidence to support that claim.



Quote:

bodhisatta said:
Quote:

bodhisatta said:
Clones are often made of multiple strains their performance may come from the interactions of the contained genetics.

http://www.tandfonline.com/doi/pdf/10.1271/bbb.67.100
Check page 5

Quote:

bodhisatta said:
there's good strains but strains are the resultant of two spores mating. so you can have good or shit strains of any variety(what a vendor calls a strain) a variety is like GT B+ etc... so really it's up to your agar work to obtain a good strain of any of the available varieties


you might have 100s of strains in a single cake if you injected spores into it. some of the mushrooms are made of more than a few strains themselves.

Quote:

bodhisatta said:


here's some nameko fruits that are displaying some white some red and some mixed. the ones in the middle are strains mixing via a process called anastomosis. the resulting fruits are called chimeric




like these nameko mushrooms with some white some red and some mushrooms spotted as the strains both have some phenotype differences manifesting in the ones with multiple strains 







:shrug:




Have a better explanation? It's debatable yes but it's going to take something very compelling to explain this in a different way


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Offlinepoisoned
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Re: How long can I keep cloning? [Re: bodhisatta]
    #26617521 - 04/21/20 11:23 AM (3 years, 9 months ago)

Thanks!


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OfflineLikeMyc
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Re: How long can I keep cloning? [Re: poisoned]
    #26617635 - 04/21/20 12:15 PM (3 years, 9 months ago)

:waitnonevermind:


Edited by LikeMyc (04/21/20 06:23 PM)


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