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OfflineZombie-ant
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Any idea what the dark thing is in the mycelium * 1
    #26563445 - 03/28/20 03:08 PM (3 years, 9 months ago)

It just appeared...
Is it contam?


Clearer on the base of the dish


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Offlinejbgtaa
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Re: Any idea what the dark thing is in the mycelium [Re: Zombie-ant] * 1
    #26563447 - 03/28/20 03:10 PM (3 years, 9 months ago)

Doesn’t look good, probably needs 24 more hours to be certain though


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OfflineZombie-ant
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Re: Any idea what the dark thing is in the mycelium [Re: jbgtaa] * 1
    #26563453 - 03/28/20 03:12 PM (3 years, 9 months ago)

Another pic


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OfflineSockadin
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Re: Any idea what the dark thing is in the mycelium [Re: Zombie-ant] * 1
    #26563583 - 03/28/20 04:08 PM (3 years, 9 months ago)

It's mold.


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OfflineZombie-ant
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Re: Any idea what the dark thing is in the mycelium [Re: Sockadin]
    #26563731 - 03/28/20 05:13 PM (3 years, 9 months ago)

Quote:

Sockadin said:
It's mold.





cut the mould out or cut all the good stuff and transfer?


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Offlinebw86
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Re: Any idea what the dark thing is in the mycelium [Re: Zombie-ant] * 1
    #26563740 - 03/28/20 05:16 PM (3 years, 9 months ago)

Is it under the agar? are you positive you didn't somehow just get some debris in before pcing?


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OfflineLogicaL ChaosM
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Re: Any idea what the dark thing is in the mycelium [Re: Zombie-ant]
    #26563776 - 03/28/20 05:28 PM (3 years, 9 months ago)

It looks like its under the agar, which would be basically impossible for mold if you PC'd your agar right. Maybe its a piece of paper that got burnt or something :shrug:


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Invisiblewoofwoof
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Re: Any idea what the dark thing is in the mycelium [Re: LogicaL Chaos]
    #26563780 - 03/28/20 05:29 PM (3 years, 9 months ago)

i would transfer out a clean piece just to be safe


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OfflineZombie-ant
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Re: Any idea what the dark thing is in the mycelium [Re: woofwoof]
    #26563903 - 03/28/20 06:24 PM (3 years, 9 months ago)

Quote:

woofwoof said:
i would transfer out a clean piece just to be safe



The majority?


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Offlineshevanel
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Re: Any idea what the dark thing is in the mycelium [Re: Zombie-ant]
    #26563936 - 03/28/20 06:40 PM (3 years, 9 months ago)

Ya

Or dont and let it keep u up at night


Edited by shevanel (03/28/20 06:40 PM)


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InvisibleRoger Clemency
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Re: Any idea what the dark thing is in the mycelium [Re: Zombie-ant]
    #26563949 - 03/28/20 06:46 PM (3 years, 9 months ago)

Quote:

Zombie-ant said:
Quote:

woofwoof said:
i would transfer out a clean piece just to be safe



The majority?




No just a piece from a clean area. You don’t need to try to salvage as much as possible to transfer just a little bit will do ya fine


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Offlinejbgtaa
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Re: Any idea what the dark thing is in the mycelium [Re: Zombie-ant]
    #26563983 - 03/28/20 07:06 PM (3 years, 9 months ago)

Quote:

Zombie-ant said:
Quote:

woofwoof said:
i would transfer out a clean piece just to be safe



The majority?



No cut an extremely small piece off, like maybe 2 grains of rice-sized and transfer. I wouldn’t put that culture to grain until you transfer it once and make sure whatever your seeing doesn’t pop up again in another plate.

That said it does look like it’s under the agar. I’d take a super tiny transfer and make sure whatever ur seeing doesn’t pop up on your next plate, then put that culture to drain.


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Offlineshevanel
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Re: Any idea what the dark thing is in the mycelium [Re: jbgtaa] * 1
    #26564152 - 03/28/20 08:40 PM (3 years, 9 months ago)

Ha! When he said majority i thought he was asking if the comment to transfer would be a majority ruling lol


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