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Ovoidhunter
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Usable mycelium or contam?
#26556317 - 03/25/20 03:49 AM (3 years, 10 months ago) |
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These are three plates knocked up with different varieties. Spores put too agar 4 days ago. Is this normal amount of time to see this kind of growth or is it contam.


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poisoned
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Re: Usable mycelium or contam? [Re: Ovoidhunter]
#26556344 - 03/25/20 04:38 AM (3 years, 10 months ago) |
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#1 looks contaminated, #2 and #3 could be normal growth for 4th day.
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Ovoidhunter
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Re: Usable mycelium or contam? [Re: poisoned]
#26556359 - 03/25/20 04:54 AM (3 years, 10 months ago) |
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Keep in mind I used a shit ton of spores definetly way more than nessesary..

I'm thinking that they all germinated on top of one another. I just read a post where someone had germination like that in 7 days. Hopefully somethings useful here.. Would like to start some grains.
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poisoned
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Registered: 04/17/13
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Re: Usable mycelium or contam? [Re: Ovoidhunter]
#26556407 - 03/25/20 06:26 AM (3 years, 10 months ago) |
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It's hard to tell from those photos if it's cube myc or sth else. You have some very vigorous spores if it has germinated and grown that much in such a short amount of time.
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Ovoidhunter
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Re: Usable mycelium or contam? [Re: poisoned]
#26556416 - 03/25/20 06:35 AM (3 years, 10 months ago) |
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I know I couldn't get the clearest pics. I'll update as they progress.
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The Mycologist
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Re: Usable mycelium or contam? [Re: Ovoidhunter]
#26556417 - 03/25/20 06:39 AM (3 years, 10 months ago) |
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I think they could use a transfer.
You can definitely clean that up.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Ovoidhunter
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I'll probably do a few then.. Thanks for the feedback. You can tell there is some sectoring on them. I've never successfully got myc on agar yet but I'm pretty sure it's myc. Will transfer to about 10 more dishes.
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The Mycologist
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Re: Usable mycelium or contam? [Re: Ovoidhunter] 1
#26556512 - 03/25/20 08:03 AM (3 years, 10 months ago) |
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Some people try to do 3 transfers before inoculation.
It may be a bit overkill, but clean spawn is worth it.
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

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Ovoidhunter
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Just did transfers to pasty plates. When I was done I opened the one shown above up outside the SAB and smelled it, smelled like mushrooms. The next one I transferred from not shown in the pictures above didn't smell very good.
May make a few more germ plates with the pasty's.
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Ovoidhunter
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Re: Usable mycelium or contam? [Re: Ovoidhunter]
#26562332 - 03/28/20 12:06 AM (3 years, 10 months ago) |
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Well i convinced myself this one out of my ten t1 plates had slime bacteria in it so i opened it up. It was just water.

All have simular growth.
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Ovoidhunter
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Re: Usable mycelium or contam? [Re: Ovoidhunter]
#26565303 - 03/29/20 01:22 PM (3 years, 10 months ago) |
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Update, these are T1s just did T2s .. one more set of transfers and they are going to grain. Should I do multiple wedges or do a whole puck and then G2G for my jars?
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Ovoidhunter
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Re: Usable mycelium or contam? [Re: Ovoidhunter]
#26619034 - 04/22/20 12:02 AM (3 years, 9 months ago) |
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Little update.
  
Im runing T5s right now when these colonize im gonna spawn a bulk tub. I have this one right now and am using those two half pints for a tub im gonna spawn tonight. Gonna drop all these plates to grains soon and its gonna be epic. Everything originated from the jar in the OP someone called contam on.
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