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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures

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Offlinedarthharper
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Registered: 11/19/19
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Contamination? Liquid culture inoculation.
    #26558510 - 03/26/20 07:22 AM (3 years, 10 months ago)

Inoculated these 3 days ago with APE liquid culture. The blue tint is concerning me. This growth is only in the inoculation areas. Thoughts?





Edited by darthharper (03/26/20 07:23 AM)


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Offlinepoisoned
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I'm a teapot User Gallery


Registered: 04/17/13
Posts: 1,738
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Re: Contamination? Liquid culture inoculation. [Re: darthharper]
    #26558541 - 03/26/20 07:52 AM (3 years, 10 months ago)

You have a liquid multi-culture.

Throw that away.


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How I do glass dishes


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Offlinedarthharper
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Re: Contamination? Liquid culture inoculation. [Re: poisoned]
    #26558546 - 03/26/20 07:55 AM (3 years, 10 months ago)

Could you please explain? Newb here.


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Offlinepoisoned
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Registered: 04/17/13
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Re: Contamination? Liquid culture inoculation. [Re: darthharper]
    #26558569 - 03/26/20 08:07 AM (3 years, 10 months ago)

This is not mycelium. Your LC is contaminated.

LCs are not the best way for us, noobs. Go make some agar. Once you can reliably make clean plates and do clean transfers, you're probably ready for LCs.

Agar is easy.


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How I do glass dishes


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Offlinedarthharper
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Re: Contamination? Liquid culture inoculation. [Re: poisoned]
    #26558575 - 03/26/20 08:10 AM (3 years, 10 months ago)

I bought a LC syringe. Not my own LC.


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Offlinepoisoned
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Re: Contamination? Liquid culture inoculation. [Re: darthharper]
    #26558581 - 03/26/20 08:16 AM (3 years, 10 months ago)

Someone probably sold you an LC made in 5min at home from spores.


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How I do glass dishes


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Offlinemarkfive67
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Re: Contamination? Liquid culture inoculation. [Re: poisoned]
    #26558713 - 03/26/20 09:31 AM (3 years, 10 months ago)

its always better when getting any syringe to put it on agar and transfer away from contaminants ....most recommend 3 to 5 transfers . it teaches you what to look for and dramatically increases the chances of success when inoculating grains. they call it agar wedge inoculation. what you have in your picture is contamination. its pretty rare that a syringe is free of contaminants. hope this helps


Edited by markfive67 (03/26/20 09:32 AM)


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OfflineThe Mycologist
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Re: Contamination? Liquid culture inoculation. [Re: markfive67]
    #26558719 - 03/26/20 09:33 AM (3 years, 10 months ago)

MOLD CITY


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That the powerful play goes on, and you will contribute a verse.”
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OfflineBallzagna
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Registered: 10/09/19
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Re: Contamination? Liquid culture inoculation. [Re: darthharper]
    #26558724 - 03/26/20 09:35 AM (3 years, 10 months ago)

I'd also stay away from corn. That's what my sister tried to use the first time, and it didn't go well. The volume to surface area isn't as good as other grains and in my experience colonized super slowly


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No one likes a naysayer

It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine.
PE Adventure
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Ballzagna's Tek Compendium and Notes for Noobs




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OfflineA.k.aM
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Re: Contamination? Liquid culture inoculation. [Re: Ballzagna]
    #26558758 - 03/26/20 09:51 AM (3 years, 10 months ago)

You need either agar or brf paste to clean it up with.


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LAGM2020


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