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InvisiblePiptheGreAtest
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LI isssues
    #26557606 - 03/25/20 06:31 PM (3 years, 9 months ago)

So I have now knock up around 8 bags with LI and about 5 jars and have yet to have growth in any of them. I’ve used the blender and blenderless technique, failing with both. I have very clean dishes ( Transfer 4-5 times from MS syringe), I pc around 150ml of water follow sterile tek and just receive no growth I mean not even contamination. Can anyone clue me in on some insight?


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InvisibleTranscendingLife
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Re: LI isssues [Re: PiptheGreAtest]
    #26557799 - 03/25/20 08:20 PM (3 years, 9 months ago)

Sounds like a moisture issue in your grains.
What’s your tek for that?


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InvisiblePiptheGreAtest
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Re: LI isssues [Re: TranscendingLife]
    #26557833 - 03/25/20 08:36 PM (3 years, 9 months ago)

Really that can cause there to be no growth. I have been switching grains around due to growth issues in spawn bags to white millet after getting the grain I absolutely hate it and will not get it again. Before I was using oats following directions of BODs easy AF oat prep for producer prides whole oats I current have 14 bags just shaken that recovered in a day without contamination. So I figure my prep is right for slurries how much difference can it make with the LI?


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InvisibleTranscendingLife
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Re: LI isssues [Re: PiptheGreAtest]
    #26557846 - 03/25/20 08:42 PM (3 years, 9 months ago)

If your grains are too dry, you wont achieve growth, no.
Are you saying “L” “I” or “L” “L” ?


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisiblePiptheGreAtest
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Re: LI isssues [Re: TranscendingLife]
    #26557884 - 03/25/20 09:06 PM (3 years, 9 months ago)

LI. So I’ve decide to try LC, are you cumulative with how many ml you need per quart of grain?


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InvisibleTranscendingLife
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Re: LI isssues [Re: PiptheGreAtest]
    #26557888 - 03/25/20 09:09 PM (3 years, 9 months ago)

I don’t recommend LCs. Go to agar before that and make sure the culture is clean.
But you do you. I’ve done plenty of LCs and had success.
I used 2-3 ML of LC per quart when I was using them.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineAtmozFear
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Re: LI isssues [Re: TranscendingLife]
    #26557912 - 03/25/20 09:26 PM (3 years, 9 months ago)

Quote:

TranscendingLife said:
I don’t recommend LCs. Go to agar before that and make sure the culture is clean.
But you do you. I’ve done plenty of LCs and had success.
I used 2-3 ML of LC per quart when I was using them.




Why don't you recommend LC's?


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InvisibleTranscendingLife
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Re: LI isssues [Re: AtmozFear]
    #26557922 - 03/25/20 09:33 PM (3 years, 9 months ago)

Not from spores, especially. But it’s extremely hard to spot contams in.
I’m on the agar—>water—>grains tek now.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisibleSmartattack
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Re: LI isssues [Re: TranscendingLife]
    #26557932 - 03/25/20 09:43 PM (3 years, 9 months ago)

I been doing lots of LC lately and I feel like Im over it.  Ill stick to it with my edibles that need mass inoculation but with all the B.S. involved, I feel like well done agar drops are not enough slower to justify the extra BS.  Not to mention the testing to make sure before use.  Just make good masters and G2G to the moon I say.


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InvisiblePiptheGreAtest
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Re: LI isssues [Re: Smartattack]
    #26557942 - 03/25/20 09:53 PM (3 years, 9 months ago)

Ok so I have almost 100 clean agar plates so it’s not from spore syringe I should of included that. I mean I have repeated failures with the Li so I figure I can skip the jars go straight to bags with the LC. This removes a step for me which is huge, I do like 30 bags at a time. I’m up for any advice on the speediest way to complete the process, I just have to use a lot of agar plates to do that many bags of spawn. I seen someone recommend 10cc to qt so that be like 50cc to a bag that seems like a lot?


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Edited by PiptheGreAtest (03/25/20 09:55 PM)


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InvisibleTranscendingLife
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Re: LI isssues [Re: PiptheGreAtest]
    #26557944 - 03/25/20 09:54 PM (3 years, 9 months ago)

To each their own, I don’t feel that agar is a “waste of time.”
Ensuring you have a clean culture is extremely important.
Quote:

PiptheGreAtest said:
Ok so I have almost 100 clean agar plates so it’s not from spore syringe I should of included that. I mean I have repeated failures with the Li so I figure I can skip the jars go straight to bags with the LC. This removes a step for me which is huge, I do like 30 bags at a time.




I’m with that. Or hit up a jar w/ the clean agar and do a GLC


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineAtmozFear
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Re: LI isssues [Re: TranscendingLife]
    #26557946 - 03/25/20 09:54 PM (3 years, 9 months ago)

I think I just don't understand LC's... I skipped that and went right to LI's.  I carved out clean agar culture, put it in PC'd distilled water, and blended it.  And then of course sucked it up with 18 gauge needles.


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InvisibleSmartattack
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Re: LI isssues [Re: TranscendingLife]
    #26557954 - 03/25/20 09:58 PM (3 years, 9 months ago)

Quote:

TranscendingLife said:
To each their own, I don’t feel that agar is a “waste of time.”
Ensuring you have a clean culture is extremely important.
Quote:

PiptheGreAtest said:
Ok so I have almost 100 clean agar plates so it’s not from spore syringe I should of included that. I mean I have repeated failures with the Li so I figure I can skip the jars go straight to bags with the LC. This removes a step for me which is huge, I do like 30 bags at a time.




I’m with that. Or hit up a jar w/ the clean agar and do a GLC





These pics hurt my heart .

:judyfacepalm:


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InvisibleTranscendingLife
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Re: LI isssues [Re: AtmozFear]
    #26557962 - 03/25/20 10:02 PM (3 years, 9 months ago)

Agar—>sterile water—>grain.
Skip the blender and just drop your agar into a sterile water with an unmodified lid.
Beat the shit outta it outside your SAB then inoculate your receiving jars. 5-6 quarts per 100ML of water/agar/myc mixture.

Smart, I got more. Just pop in my gallery. Lol. I’ve seen almost all of them


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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InvisiblePiptheGreAtest
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Re: LI isssues [Re: TranscendingLife]
    #26557974 - 03/25/20 10:10 PM (3 years, 9 months ago)

Quote:

TranscendingLife said:
To each their own, I don’t feel that agar is a “waste of time.”
Ensuring you have a clean culture is extremely important.
Quote:

PiptheGreAtest said:
Ok so I have almost 100 clean agar plates so it’s not from spore syringe I should of included that. I mean I have repeated failures with the Li so I figure I can skip the jars go straight to bags with the LC. This removes a step for me which is huge, I do like 30 bags at a time.




I’m with that. Or hit up a jar w/ the clean agar and do a GLC




GLC? Just a different broth from LME?


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InvisiblePiptheGreAtest
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Re: LI isssues [Re: TranscendingLife]
    #26557980 - 03/25/20 10:13 PM (3 years, 9 months ago)

Quote:

TranscendingLife said:
Agar—>sterile water—>grain.
Skip the blender and just drop your agar into a sterile water with an unmodified lid.
Beat the shit outta it outside your SAB then inoculate your receiving jars. 5-6 quarts per 100ML of water/agar/myc mixture.

Smart, I got more. Just pop in my gallery. Lol. I’ve seen almost all of them



Honestly wish I could do it that way, I’ve tried the blenderless and blender versions I just can’t get anything to grow with it. I mean I did fine with slurries that’s along same line. Super frustrated with it because since I e been cultivating all I’ve heard is LC hides contaminates and there are better inculcation methods. But I mean with doing 30 spawn bags at a time I’m not going wait on 30 jars when there are known methods to go straight to the bag.


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InvisibleTranscendingLife
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Re: LI isssues [Re: PiptheGreAtest]
    #26557985 - 03/25/20 10:16 PM (3 years, 9 months ago)

GLC you take a fully colonized jar and shoot sterile water into it.
Shake the hell outta it to get the myc off the grains.
Then suck up the water into the syringe.
After that you can transfer it to LC.
But it’s still not a guarantee of  a clean culture.
Link

Bags and jars are the same thing as long as we’re talking spawn and sterile technique.
Bags you would just use more to inoculate than a jar. Just multiply the ratios.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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Invisiblec10h12n2o
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Re: LI isssues [Re: TranscendingLife]
    #26558032 - 03/25/20 11:00 PM (3 years, 9 months ago)

Personally I love LCs, scales so well and fast af. I typically inoculate the lc with agar or LI, leave it on a stir plate about 12 hrs a day, and its usually ready to use 3-7 days later. Definitely not a good idea to use spores. I typically use 6-10 ml for jars and 30-50ml per bag. Full colonization in 4-8 days.

Yeah its definitely riskier (eggs in one basket, cant spot contams) but if your cultures are clean and your clean tek is on point, the benefits are great. I havent had an lc contam in probably 10 yrs, but I'm kinda rediculous w my clean tek

Your problem with li might be your grain prep, moisture content etc.

No growth after how many days? Li will have way less myc per ml than an lc, so I'd expect it to be slower unless it's very low volume LI and high volume injection


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