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Snowtrooper
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My LC test
#26557314 - 03/25/20 03:39 PM (3 years, 9 months ago) |
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Wats up This is my LC test In the top row I have isolated the strain a bit in the bottom I have the original (I took MS and cloned it 2 times then made a LC)
A couple of thing I need to know, should I use the bottom row LC or just use the top row LC because it looks much better on the agar.
Second it seems to me the LC is not contaminated is it too early to tell or does this look kosher.
Thank you for your input as always this forum is a life saver for beginners.
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mimir
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Looks fine to me, you could open the plates and smell them, bacterial contamination is not only visible but definitely smells badly. You need to share your lighting setup because pics are dope
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Strainsfordaze


Registered: 05/10/18
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Re: My LC test [Re: mimir] 1
#26557368 - 03/25/20 04:17 PM (3 years, 9 months ago) |
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that is poor advise. do not open them outside of a flowhood or sab unless you don't want to use them in the future. I would use one of the Lcs with one of the plates from the top row.
Edited by Strainsfordaze (03/25/20 08:11 PM)
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Sockadin



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You have mold in every plate. Mycelium aswell, but you definitely have grown mold.
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mimir
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Quote:
Strainsfordaze said: that is poor advise. do not open them outside of a flowhood or sab unless you don't want to use them in the future. I would use one of the Lcs that was inoculated with one of the plates from the top row.
He said it was a LC test. Why would he use them if he has LC, they obviously are for the sole purpose of determining the cleanliness of the LC
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Sockadin



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Re: My LC test [Re: mimir]
#26557400 - 03/25/20 04:34 PM (3 years, 9 months ago) |
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Maybe it's a dimple in every lid.. hell I'm not sure anymore.
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mimir
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Here's some mold
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Sockadin



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Re: My LC test [Re: mimir]
#26557412 - 03/25/20 04:40 PM (3 years, 9 months ago) |
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Thank man, I didn't really know what mold looked like and was just making things up. I appreciate you sharing.
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Snowtrooper
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Hey bro So I def have mold because of the fuzzy stuff in the middle or what wouldn't mold spread throughout the plate or is it just plain to see on every plate
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Snowtrooper
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Its sometimes tricky to see what's mold and what's just fuzzy myc but if so its probably best to start from scratch right just take a nice looking shroom and get a sample in agar or should I take a sector from these plates and go from there?
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Snowtrooper
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Re: My LC test [Re: mimir]
#26557628 - 03/25/20 06:47 PM (3 years, 9 months ago) |
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Its just a fluorescent lamp that sits on the workstation edge pretty basic
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mimir
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Re: My LC test [Re: Sockadin] 1
#26557674 - 03/25/20 07:15 PM (3 years, 9 months ago) |
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Quote:
Sockadin said: Thank man, I didn't really know what mold looked like and was just making things up. I appreciate you sharing.
Dimples caught my eye too, I think that's just the plastic though 
Quote:
Snowtrooper said: Hey bro So I def have mold because of the fuzzy stuff in the middle or what wouldn't mold spread throughout the plate or is it just plain to see on every plate
I don't think you have mold, but I'm not an expert on the matter, there certainly exist many different kinds of mold. The mold I grew looks nothing like your plates, the edges are completely different and the texture too. It fooled me to the point that I put it to grains since I didn't know better, but I don't think it's your case. Also, it didn't smell like anything, certainly not like mushrooms, which your plates should (hence my suggestion). If you disturb the mycelium with a finger you will be able to clearly smell it. Goes without saying that you'll throw away the contents of the dish after that, but it's for the purpose of testing your LC. To me it looks like very good looking mycelium. The ones on the bottom are less obvious but growing them out will probably show them to be mycelium too. Here's a thread that might prove helpful:
How it should & shouldn't look - NEW CULTIVATORS GUIDE
Hope I was helpful, happy growing!
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A.k.a
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Re: My LC test [Re: mimir]
#26557727 - 03/25/20 07:44 PM (3 years, 9 months ago) |
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Sock where are you seeing mold in the top row?
Op I’d use the more isolated version the bottom row doesn’t look very vigorous and the tops got some nice rhizo.
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LAGM2020     
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TranscendingLife
I Don't Need a Life to Live



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Re: My LC test [Re: A.k.a]
#26557794 - 03/25/20 08:18 PM (3 years, 9 months ago) |
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Top three are golden. You can try to clean up the bottom three a bit. I’d take the top right one and transfer that to another plate. Looks almost perfect.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Sockadin



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Re: My LC test [Re: A.k.a]
#26557811 - 03/25/20 08:27 PM (3 years, 9 months ago) |
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Quote:
A.k.a said: Sock where are you seeing mold in the top row?
Op I’d use the more isolated version the bottom row doesn’t look very vigorous and the tops got some nice rhizo.
3:00. 12:00 and 11;00 but I'm thinking it's the dish indent now and not mold. And it's Coronavirus vacation so I been maybe has some beers.
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TranscendingLife
I Don't Need a Life to Live



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Sock, you’re definitely seeing the Petri dish lid there.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Snowtrooper
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Re: My LC test [Re: A.k.a] 1
#26557817 - 03/25/20 08:29 PM (3 years, 9 months ago) |
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Hey guys thank you all for your input...there seems to be a little debate on the mold situation so here are a few jars I inoculated with the LC in the top row. I inoculated them on the 13. of this month so about 2 weeks ago I'm not sure how they are going to end up its too early to tell but I've been monitoring them and they look okay to my beginner eyes, I don't know if it would be visible or not by now if they were contaminated. plese tell me what you think.
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Sockadin



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They look really healthy. My bad. Glad no one listened to me on that post!
Rye looks good too. Can't wait to see it fruit
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TranscendingLife
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Re: My LC test [Re: Sockadin] 1
#26557825 - 03/25/20 08:33 PM (3 years, 9 months ago) |
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Please don’t thread jack. And Shake those bitches!
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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PiptheGreAtest
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If you don’t mind, what grain prep and LC tek are you using? I’m having difficulty with LI and might just try to make some LC S seems to go pretty fast and that’s what I’m looking for. Not to hijack the tread can you use LC on 5qt spawn bags?
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Snowtrooper
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Me too bro ill post the pics
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Snowtrooper
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hey bro no problem So for the grain im using whole wheat grains 1st class I bought from a retailer bulk I rinse them and let them sit in water for 24 hours and then boil for 15min then I let them air dry and put them in jars for PC 15psi 90min
The LC I PC tap water with honey in it (1 tsp) then just pick a small good spot from the agar plate to inoculate and use a magnetic stirrer for 5-10 min a day it takes a week maybe to get there.
I've done bags and jars with no problem
Edited by Snowtrooper (03/25/20 09:08 PM)
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TranscendingLife
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You shake those jars yet!?
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Snowtrooper
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Hey my man wasn't jacking the thread good sir, these jars are from the LC we were talking about, the top row I thought it would help to see the LC we were talking about in jars
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TranscendingLife
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I realized that. Sorry brother. I’ve got a buzz on too. Multitasking isn’t my strong suit unless I’m behind the bar.  Now go shake those jars, damn it.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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Snowtrooper
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Yeah the thing is im waiting on my mason jars they are in the mail, in the mean time im using jars I don't trust I think the last time I shook it I invited contaminants in so now im just letting them sit, I know it will take a little more time to colonize but its a little safer
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TranscendingLife
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Jars are jars  It’d have to be your GE filter or lid rings that are bad.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
      How I Do EVERYTHING      "Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung "Anything that can be done chemically can be done by other means."- William S. Burroughs "You are as dead now as you will ever be" - Seth
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WeavieWonder
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Quote:
Snowtrooper said: Hey guys thank you all for your input...there seems to be a little debate on the mold situation so here are a few jars I inoculated with the LC in the top row. I inoculated them on the 13. of this month so about 2 weeks ago I'm not sure how they are going to end up its too early to tell but I've been monitoring them and they look okay to my beginner eyes, I don't know if it would be visible or not by now if they were contaminated. plese tell me what you think.

Testing LC on grain is the best gauge of cleanliness IMO. Mold can be sneaky. Those jars look great now, but don't start getting excited until they're 100%, and have sit around for a couple days. The fact that its been two weeks and them jars are still not finished is a sign that something aint right.
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Sockadin



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No true, some time jars can take longer. Especially if there was no initial shake after Inoculation.
Shaking jars won't increase contams unless there are bad lids or filters. If you shake and get a contamination, usually it was there to begin with.
TL is right, time to shake.
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Snowtrooper
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Hey bro yes that's true im not there by a long shot, contamination can lurk in there but these jars are 67oz so it will take a little longer for them to colonize than 2 weeks.
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Snowtrooper
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Quote:
TranscendingLife said: Top three are golden. You can try to clean up the bottom three a bit. I’d take the top right one and transfer that to another plate. Looks almost perfect.
Thank you bro ill do that
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mimir
Professional Noob

Registered: 01/21/19
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Quote:
Snowtrooper said: Hey guys thank you all for your input...there seems to be a little debate on the mold situation so here are a few jars I inoculated with the LC in the top row. I inoculated them on the 13. of this month so about 2 weeks ago I'm not sure how they are going to end up its too early to tell but I've been monitoring them and they look okay to my beginner eyes, I don't know if it would be visible or not by now if they were contaminated. plese tell me what you think.

Are those lids airtight or did you drill a hole in the glass? Lack of GE could make them stall
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A.k.a
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Re: My LC test [Re: mimir]
#26558712 - 03/26/20 09:31 AM (3 years, 9 months ago) |
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I don’t think two weeks with no shake is that long.
Those look really good I bet you shake them and they’re done in two days.
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LAGM2020     
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