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OfflineMykology
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Registered: 01/23/20
Posts: 92
Last seen: 2 years, 1 month
Agar to Grain Transfer
    #26556264 - 03/25/20 01:32 AM (3 years, 9 months ago)

Hello Shroomery,
        I’ve seen some conflicting information so I figured I would ask all you wise sages.  What size cubes do you utilize to transfer agar culture to grain?  Also, is it better to utilize a whole third of a Petri culture, or cut smaller cubes so there are more inoculation points?
                                          -Mykology


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OfflineIgnorantape
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Registered: 01/30/20
Posts: 149
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Re: Agar to Grain Transfer [Re: Mykology]
    #26556338 - 03/25/20 04:36 AM (3 years, 9 months ago)

I've had success so far using the whole plate. I just shake it up after inoculating the grain. That breaks up the disk of agar and mycelium and disperses the myc throughout the jar, leaving multiple inoculation points. You can get away with using a smaller amount of myc but it will take longer to colonize.


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Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
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Re: Agar to Grain Transfer [Re: Ignorantape]
    #26556550 - 03/25/20 08:23 AM (3 years, 9 months ago)

It doesn't matter what part of the dish you cut from to goto grain. Some
people like using the leading edge of the growth cause its more active.
I personally cut thumbnail sized squares for each jar so I can do a bunch
of jars with one dish. But I only light swirl the jar around afterwards
to get the square buried under the surface where it wont dry out as much.
Then shake the jar in two week, then it will finish colonizing really quickly


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InvisibleSmartattack
C'mon man
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Registered: 12/21/18
Posts: 3,775
Loc: A thought
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Re: Agar to Grain Transfer [Re: rumfor69]
    #26556661 - 03/25/20 09:25 AM (3 years, 9 months ago)

I like 3-4 fingernail sized pieces distributed into the grain to make masters. I've tried whole plates as well but it sucks when it sticks to the side of the jar.


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Agar to Grain Transfer [Re: Smartattack]
    #26556669 - 03/25/20 09:31 AM (3 years, 9 months ago)

it doesn't really matter just keep your goal in mind, using an entire puck may become a pita at g2g for example


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