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SwellGuy
Stranger

Registered: 02/15/20
Posts: 7
Last seen: 3 years, 9 months
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Ready for FAE?
#26555073 - 03/24/20 12:40 PM (3 years, 10 months ago) |
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Hello!
This is my first post here after being a long, long time lurker and having to make a new account since I lost access to my old email (I didnt post there either so nbd)
So, I have lurked far and wide but still want to just be sure because reality is better than pictures.
Do yall think this is ready for FAE? I see slight yellowing on my mycelium in some places - looks more like metab than contam - and i hear over-humidity can cause slight yellowing.
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Ready for FAE? [Re: SwellGuy] 1
#26555097 - 03/24/20 12:54 PM (3 years, 10 months ago) |
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Hell yeah fruit it before it goes green.
That spot in the middle bottom is concerning but other than that looks good.
-------------------- Tek's I use LAGM2020
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SwellGuy
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Registered: 02/15/20
Posts: 7
Last seen: 3 years, 9 months
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Yeah to be 100% I have no idea why it isnt colonizing there.
Doesnt look moldy or anything, just... not colonized. Those are rather dense pieces of wood chips though!
Watching it closely - may sterilize my long tweezers and remove some possible contams later (My plan: Torch the lower half, douse in Everclear)
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Ready for FAE? [Re: SwellGuy] 1
#26555111 - 03/24/20 01:01 PM (3 years, 10 months ago) |
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No need. Your already fruiting in an unsterile environment.
Just leave it and hope your flush finishes before it takes over.
-------------------- Tek's I use LAGM2020
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SwellGuy
Stranger

Registered: 02/15/20
Posts: 7
Last seen: 3 years, 9 months
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Quote:
staytrippy420 said: No need. Your already fruiting in an unsterile environment.
Just leave it and hope your flush finishes before it takes over. 
What makes it an unsterile environment? It was fully (EDIT: Sanitized), the air in this room is filtered (not that it's a big issue around my area) and every product involved was double-sterilized with H202 then 90% Ethanol
But I do see what you mean and thank you for the advice
Edited by SwellGuy (03/24/20 03:34 PM)
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staytrippy420


Registered: 03/23/13
Posts: 2,337
Loc: Canada
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Re: Ready for FAE? [Re: SwellGuy] 1
#26555153 - 03/24/20 01:30 PM (3 years, 10 months ago) |
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1. The air we breath is not sterile. The moment you spawned your jars you introduce it to an unsterile environment. That’s why we pressure cook jars. To creat a sterile environment inside the jars. Once you break the seal it’s no longer sterile.
2. That spot is most likely a contam.. How can it be sterile environment if there a contam in your tub?
-------------------- Tek's I use LAGM2020
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LookingLurker
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Registered: 01/03/20
Posts: 47
Last seen: 1 year, 3 months
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Re: Ready for FAE? *DELETED* [Re: SwellGuy]
#26555187 - 03/24/20 01:59 PM (3 years, 10 months ago) |
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Post deleted by LookingLurker
Reason for deletion: .
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Morbility
Cybernetic Psychonaut


Registered: 12/04/18
Posts: 166
Loc: Ilus IV
Last seen: 1 year, 7 months
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Correct terminology is important here. H2O2 isn't useful for just about anything in this hobby aside from some real edge case issues that don't apply to your circumstances here. 90% iso is less effective than 70% and it's a sanitizing agent. As staytrippy420 said, nothing is sterile outside of the jars.
Moving on... that bottom patch looks a little sketchy, but tough to tell. I had an area of coir in my last monotub that just didn't want to do shit, but my tub is still fruiting and nothing came of it, so you'll have to just watch it and be diligent. Judging by the myc climbing at the edges and the fluffiness, FAE could definitely help you out.
Beyond that, what are you using for substrate and grain? What ratio did you use going to bulk? Who's teks are you following for this project?
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SwellGuy
Stranger

Registered: 02/15/20
Posts: 7
Last seen: 3 years, 9 months
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Quote:
Morbility said:

Correct terminology is important here. H2O2 isn't useful for just about anything in this hobby aside from some real edge case issues that don't apply to your circumstances here. 90% iso is less effective than 70% and it's a sanitizing agent. As staytrippy420 said, nothing is sterile outside of the jars.
Moving on... that bottom patch looks a little sketchy, but tough to tell. I had an area of coir in my last monotub that just didn't want to do shit, but my tub is still fruiting and nothing came of it, so you'll have to just watch it and be diligent. Judging by the myc climbing at the edges and the fluffiness, FAE could definitely help you out.
Beyond that, what are you using for substrate and grain? What ratio did you use going to bulk? Who's teks are you following for this project?
This is the monotub tek that I will try to get a link for, I forget the author's name.
For substrate and grain I used mycohaus as my pressure cooker decided to break lately. MycoHaus has the bag with both grain and substrate in it. Horse manure based substrate with rye grain.
Also, just so you know - as a chemist, 70-90% alcohol are both very effective. 90%+ is less effective for proteins (like viruses) whereas it still has chemical potential to infertilize spores and kill bacteria. Also, ethanol works slightly different than isopropanol. But yes, sorry, Sanitizing is what i meant, not sterilization - that would imply purity.
Thank you again for the advice and info - both of you! Very nice And I apologize for my misuse in terms! I should have my coffee before posting.
Edited by SwellGuy (03/24/20 03:34 PM)
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Morbility
Cybernetic Psychonaut


Registered: 12/04/18
Posts: 166
Loc: Ilus IV
Last seen: 1 year, 7 months
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Re: Ready for FAE? [Re: SwellGuy]
#26555555 - 03/24/20 05:33 PM (3 years, 10 months ago) |
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Bummer about your PC. Some folks have good luck with mail order spawn/sub and some don't. Looks like you're in the former category from the progress so far, so that's good. Also seems like less mucking about with moisture levels (my personal thorn) as the myc appears pretty healthy overall from the look of things.
You're absolutely right about the alcohol. To be honest, once I saw the mention of H2O2 and percentages I immediately skipped past the EtOH and mentally figured iso. So I should take my own advice as well and verify terminology before I go being helpful. I'm also a bit sleep deprived, so I apologize if I came off surly.
Consensus in these parts is bacteria is the biggest problem, since fungal spores easily die during pasteurization and sanitization procedures, but more resilient nasties survive and bide their time. The bacteria then weakens the myc and that's when the parasitic molds show up and totally ruin the party. Some of the more experienced cultivators here don't bother with spraying sanitizing agents at all--I read a post of Bod's not that long ago where he states that he just flames the ring on his jars if he feels the need.
Definitely keep us updated on your progress. Everyone loves seeing successes here, especially when things are so sideways in the real world!
Edited by Morbility (03/24/20 05:34 PM)
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